Uniform tissues engineered by seeding and culturing cells in 3D scaffolds under perfusion at defined oxygen tensions

Biorheology

Volumn 43, Issue 3-4, 2006, Pages 481-488

  Wendt, D ^a   Stroebel, S ^a   Jakob, M ^a   John, G T ^b   Martin, I ^a  

^a UNIVERSITY HOSPITAL BASEL   (Switzerland)

^b PRESENS PRECISION SENSING GMBH   (Germany)

Author keywords

Bioreactor; Chondrocyte; Fluid flow; Functional tissue engineering; Mass transport

Indexed keywords

ARTICULAR CARTILAGE; BIOREACTOR; CARTILAGE CELL; CELL CULTURE; CELL SUSPENSION; CELL VIABILITY; CONFERENCE PAPER; CULTURE MEDIUM; FLUID FLOW; HUMAN; HUMAN CELL; OXYGEN TENSION; THREE DIMENSIONAL IMAGING; TISSUE ENGINEERING;

ADULT; BIOREACTORS; CARTILAGE, ARTICULAR; CELL CULTURE TECHNIQUES; CELL SURVIVAL; CHONDROCYTES; CHONDROGENESIS; EQUIPMENT DESIGN; HUMANS; MICROSCOPY, CONFOCAL; OXYGEN; PERFUSION; POROSITY; TISSUE ENGINEERING;

EID: 31844451096     PISSN: 0006355X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Conference Paper

References (8)

1. T. Davisson, R.L. Sah and A. Ratcliffe, Perfusion increases cell content and matrix synthesis in chondrocyte three-dimensional cultures, Tissue Eng. 8 (2002), 807-816.
2. N.S. Dunkelman, M.P. Zimber, R.G. LeBaron, R. Pavelec, M. Kwan and A.F. Purchio, Cartilage production by rabbit articular chondrocytes on polyglycolic acid scaffolds in a closed bioreactor system, Biotechnol. Bioeng. 46 (1995), 299-305.
3. M.C. Lewis, B.D. Macarthur, J. Malda, G. Pettet and C.P. Please, Heterogeneous proliferation within engineered cartilaginous tissue: the role of oxygen tension, Biotechnol. Bioeng. 91 (2005), 607-615.
4. J. Malda, J. Rouwkema, D.E. Martens, E.P. Le Comte, F.K. Kooy, J. Tramper, C.A. van Blitterswijk and J. Riesle, Oxygen gradients in tissue-engineered PEGT/PBT cartilaginous constructs: measurement and modeling, Biotechnol. Bioeng. 86 (2004), 9-18.
5. D. Pazzano, K.A. Mercier, J.M. Moran, S.S. Fong, D.D. DiBiasio, J.X. Rulfs, S.S. Kohles and L.J. Bonassar, Comparison of chondrogenesis in static and perfused bioreactor culture, Biotechnol. Prog. 16 (2000), 893-896.
6. M.T. Raimondi, F. Boschetti, L. Falcone, F. Migliavacca, A. Remuzzi and G. Dubini, The effect of media perfusion on three-dimensional cultures of human chondrocytes: integration of experimental and computational approaches, Biorheology 41 (2004), 401-410.
7. G. Vunjak-Novakovic, B. Obradovic, I. Martin, P.M. Bursac, R. Langer and L.E. Freed, Dynamic cell seeding of polymer scaffolds for cartilage tissue engineering, Biotechnol. Prog. 14 (1998), 193-202.
8. D. Wendt, A. Marsano, M. Jakob, M. Heberer and I. Martin, Oscillating perfusion of cell suspensions through three-dimensional scaffolds enhances cell seeding efficiency and uniformity, Biotechnol. Bioeng. 84 (2003), 205-214.