Tunable Microfibers Suppress Fibrotic Encapsulation via Inhibition of TGFβ Signaling

Tissue Engineering - Part A

Volumn 22, Issue 1-2, 2016, Pages 142-150

  Allen, Jessica ^a   Ryu, Jubin ^b   Maggi, Alessandro ^c   Flores, Bianca ^a   Greer, Julia R ^c   Desai, Tejal ^a  

^a UCSF Schools of Pharmacy and Medicine   (United States)

^b UCSF Department of Dermatology   (United States)

^c California Institute of Technology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCOMPATIBILITY; CELL CULTURE; GENE EXPRESSION;

CRITICAL FEATURES; IMPLANTABLE BIOMEDICAL DEVICES; IMPLANTED MATERIALS; MICRO TOPOGRAPHY; MICROSCALE TOPOGRAPHY; MYOFIBROBLASTS; SIGNALING COMPONENTS; SURFACE BIOCOMPATIBILITY;

TISSUE;

ALPHA SMOOTH MUSCLE ACTIN; COLLAGEN 1 ALPHA 2; COLLAGEN 3 ALPHA 1; COLLAGEN TYPE 1; COLLAGEN TYPE 3; MYOSIN LIGHT CHAIN; POLYPROPYLENE; SMAD3 PROTEIN; TRANSFORMING GROWTH FACTOR BETA; TRANSFORMING GROWTH FACTOR BETA RECEPTOR 2; TRANSFORMING GROWTH FACTOR BETA1; UNCLASSIFIED DRUG; ARTIFICIAL MEMBRANE; DIFFERENTIATION ANTIGEN; TGFB1 PROTEIN, MOUSE;

ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CELL ENCAPSULATION; CELL SHAPE; CELL STRUCTURE; CONTROLLED STUDY; FEMALE; GENE EXPRESSION; IMMUNOFLUORESCENCE; MOUSE; MYOFIBROBLAST; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; RIGIDITY; SCANNING ELECTRON MICROSCOPY; SIGNAL TRANSDUCTION; STRESS FIBER; WOUND HEALING; 3T3 CELL LINE; ANIMAL; ARTIFICIAL MEMBRANE; BIOSYNTHESIS; FIBROSIS; METABOLISM; PATHOLOGY;

3T3 CELLS; ANIMALS; ANTIGENS, DIFFERENTIATION; CELL DIFFERENTIATION; FIBROSIS; MEMBRANES, ARTIFICIAL; MICE; MYOFIBROBLASTS; SIGNAL TRANSDUCTION; TRANSFORMING GROWTH FACTOR BETA1;

EID: 84955071097     PISSN: 19373341     EISSN: 1937335X     Source Type: Journal    
DOI: 10.1089/ten.tea.2015.0087     Document Type: Article

References (39)

1. Jung, K.I., Lee, S.-B., Kim, J.H., and Park, C.K. Foreign body reaction in glaucoma drainage implant surgery. Invest Ophthalmol Vis Sci 54, 3957, 2013.
2. Dang, T.T., et al. Enhanced function of immuno-isolated islets in diabetes therapy by co-encapsulation with an antiinflammatory drug. Biomaterials 34, 5792, 2013.
3. Santos, E., Pedraz, J.L., Hernández, R.M., and Orive, G. Therapeutic cell encapsulation: ten steps towards clinical translation. J Control Release 170, 1, 2013.
4. Tomasek, J.J., Gabbiani, G., Hinz, B., Chaponnier, C., and Brown, R.A. Myofibroblasts and mechano-regulation of connective tissue remodelling. Nat Rev Mol Cell Biol 3, 349, 2002.
5. Li, A.G., et al. Elevation of transforming growth factor beta (TGF b) and its downstream mediators in subcutaneous foreign body capsule tissue. J Biomed Mater Res A 82, 498, 2007.
6. Allen, J.L., Cooke, M.E., and Alliston, T. ECM stiffness primes the TGFbeta pathway to promote chondrocyte differentiation. Mol Biol Cell 23, 3731, 2012.
7. Huang, X., et al. Matrix stiffness-induced myofibroblast differentiation is mediated by intrinsic mechanotransduction. Am J Respir Cell Mol Biol 47, 340, 2012.
8. Wipff, P.-J., Rifkin, D.B., Meister, J.-J., and Hinz, B. Myofibroblast contraction activates latent TGF-beta1 from the extracellular matrix. J. Cell Biol 179, 1311, 2007.
9. Hinz, B. The myofibroblast: paradigm for a mechanically active cell. J Biomech 43, 146, 2010.
10. Hinz, B. Tissue stiffness, latent TGF-beta1 activation, and mechanical signal transduction: implications for the pathogenesis and treatment of fibrosis. Curr Rheumatol Rep 11, 120, 2009.
11. Mateos-Timoneda, M.A., Castano, O., Planell, J.A., and Engel, E. Effect of structure, topography and chemistry on fibroblast adhesion and morphology. J Mater Sci Mater Med 25, 1781, 2014.
12. Orive, G., Tam, S.K., Pedraz, J.L., and Hallé, J.-P. Biocompatibility of alginate-poly-L-lysine microcapsules for cell therapy. Biomaterials 27, 3691, 2006.
13. Ayala, P., and Desai, T.A. Integrin a3 blockade enhances microtopographical down-regulation of a-smooth muscle actin: role of microtopography in ECM regulation. Integr Biol (Camb). 3, 733, 2011.
14. Ayala, P., Lopez, J.I., PhD, and Desai, T.A. Microtopographical cues in 3D attenuate fibrotic phenotype and extracellular matrix deposition : implications for tissue regeneration. Tissue Eng Part A 16, 2519, 2010.
15. Bettinger, C.J., Langer, R., and Borenstein, J.T. Engineering substrate topography at the micro-and nanoscale to control cell function. Angew Chem Int Ed Engl 48, 5406, 2009.
16. Clark, P., Connolly, P., Curtis, A.S., Dow, J.A, and Wilkinson, C.D. Cell guidance by ultrafine topography in vitro. J Cell Sci 99, 73, 1991.
17. Dalby, M.J., Riehle, M.O., Johnstone, H., Affrossman, S., and Curtis, A.S. Investigating the limits of filopodial sensing: a brief report using SEM to image the interaction between 10 nm high nano-topography and fibroblast filopodia. Cell Biol Int 28, 229, 2004.
18. Kam, K.R., et al. Nanostructure-mediated transport of biologics across epithelial tissue: enhancing permeability via nanotopography. Nano Lett 13, 164, 2013.
19. Kam, K.R., et al. The effect of nanotopography on modulating protein adsorption and the fibrotic response. Tissue Eng Part A 20, 130, 2014.
20. Lam, M.T., Clem, W.C., and Takayama, S. Reversible ondemand cell alignment using reconfigurable microtopography. Biomaterials 29, 1705, 2008.
21. McGarry, J.P., et al. Simulation of the contractile response of cells on an array of micro-posts. Phil Trans A Math Phys Eng Sci 367, 3477, 2009.
22. McNamara, L.E., et al. Nanotopographical control of stem cell differentiation. J Tissue Eng 18, 120623, 2010.
23. Muthusubramaniam, L., Zaitseva, T., Paukshto, M., Martin, G., and Desai, T. Effect of collagen nanotopography on keloid fibroblast proliferation and matrix synthesis : implications for dermal wound healing. Tissue Eng Part A 20, 2728, 2014.
24. Bettinger, C.J., Kulig, K.M., Vacanti, J.P., Langer, R., and Borenstein, J.T. Nanofabricated collagen-inspired synthetic elastomers for primary rat hepatocyte culture. Tissue Eng Part A 15, 1321, 2010.
25. Pot, S.A., et al. Nanoscale topography-induced modulation of fundamental cell behaviors of rabbit corneal keratocytes, fibroblasts, and myofibroblasts. Invest Ophthalmol Vis Sci 51, 1373, 2010.
26. Dalby, M., et al. Fibroblast reaction to island topography: changes in cytoskeleton and morphology with time. Biomaterials 24, 927, 2003.
27. Harvey, A.G., Hill, E.W., and Bayat, A. Designing implant surface topography for improved biocompatibility. Expert Rev Med Devices 10, 257, 2013.
28. Zhang, W., Ou, J., Inagaki, Y., Greenwel, P., and Ramirez, F. Synergistic cooperation between Sp1 and Smad3/Smad4 mediates transforming growth factor beta1 stimulation of alpha 2(I)-collagen (COL1A2) transcription. J Biol Chem 275, 39237, 2000.
29. Sysa, P., Potter, J.J., Liu, X., and Mezey, E. Transforming growth factor-b 1 up-regulation by Sp1 and Smad2 transacting factors. DNA Cell Biol 28, 425, 2009.
30. Verrecchia, F., Chu, M.L., and Mauviel, A. Identification of novel TGF-beta/Smad gene targets in dermal fibroblasts using a combined cDNA microarray/promoter transactivation approach. J Biol Chem 276, 17058, 2001.
31. Hu, B., Wu, Z., and Phan, S.H. Smad3 mediates transforming growth factor-beta-induced alpha-smooth muscle actin expression. Am J Respir Cell Mol Biol 29, 397, 2003.
32. Gu, L., et al. Effect of TGF-beta/Smad signaling pathway on lung myofibroblast differentiation. Acta Pharmacol Sin 28, 382, 2007.
33. Ghosh, A.K., Yuan, W., Mori, Y., and Varga, J. Smaddependent stimulation of type I collagen gene expression in human skin fibroblasts by TGF-beta involves functional cooperation with p300/CBP transcriptional coactivators. Oncogene 19, 3546, 2000.
34. Margadant, C., and Sonnenberg, A. Integrin-TGF-beta crosstalk in fibrosis, cancer and wound healing. EMBO Rep 11, 97, 2010.
35. Reynolds, L.E., et al. Accelerated re-epithelialization in beta3-integrin-deficient-mice is associated with enhanced TGF-beta1 signaling. Nat Med 11, 167, 2005.
36. Asano, Y., Ihn, H., Yamane, K., Jinnin, M., and Tamaki, K. Increased expression of integrin avb5 induces the myofibroblastic differentiation of dermal fibroblasts. Am J Pathol 168, 499, 2006.
37. Assoian, R.K., and Klein, E.A. Growth control by intracellular tension and extracellular stiffness. Trends Cell Biol 18, 347, 2008.
38. Lee, J., Majidi, C., Schubert, B., and Fearing, R.S. Slidinginduced adhesion of stiff polymer microfibre arrays. II. Microscale behaviour. J R Soc Interface 5, 845, 2008.
39. Livak, K.J., and Schmittgen, T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25, 402, 2001.