Homogeneous fluorescence anisotropy-based assay for characterization of ligand binding dynamics to GPCRs in budded baculoviruses: The case of Cy3B-NDP-α-MSH binding to MC4 receptors

Methods in Molecular Biology

Volumn 1272, Issue , 2015, Pages 37-50

  Veiksina, Santa ^a   Kopanchuk, Sergei ^a   Mazina, Olga ^a   Link, Reet ^a   Lille, Anne ^a   Rinken, Ago ^a  

^a NONE

Author keywords

Binding kinetics; Budded baculoviruses; Fluorescence anisotropy; Global analysis; Melanocortin 4 receptor

Indexed keywords

ALPHA INTERMEDIN; G PROTEIN COUPLED RECEPTOR; MELANOCORTIN 4 RECEPTOR; AFFINITY LABELING; LIGAND; MOLECULAR LIBRARY; MSH, 4-NLE-7-PHE-ALPHA-; PROTEIN BINDING;

ANIMAL CELL; ANISOTROPY; ARTICLE; BACULOVIRIDAE; DATA ANALYSIS; FLUORESCENCE ANALYSIS; FLUORESCENCE ANISOTROPY; LIGAND BINDING; NONHUMAN; PRIORITY JOURNAL; RECEPTOR BINDING; VIRUS CONCENTRATION; VIRUS RELEASE; AFFINITY LABELING; AGONISTS; ANALOGS AND DERIVATIVES; ANIMAL; ANTAGONISTS AND INHIBITORS; BINDING COMPETITION; BINDING SITE; BIOASSAY; CHEMISTRY; FLUORESCENCE POLARIZATION; GENE EXPRESSION; GENETICS; HUMAN; KINETICS; METABOLISM; MOLECULAR LIBRARY; PHARMACOLOGY; PLASMID; PROCEDURES; SF9 CELL LINE; SPODOPTERA;

AFFINITY LABELS; ALPHA-MSH; ANIMALS; BACULOVIRIDAE; BINDING SITES; BINDING, COMPETITIVE; BIOLOGICAL ASSAY; FLUORESCENCE POLARIZATION; GENE EXPRESSION; HUMANS; KINETICS; LIGANDS; PLASMIDS; PROTEIN BINDING; RECEPTOR, MELANOCORTIN, TYPE 4; SF9 CELLS; SMALL MOLECULE LIBRARIES; SPODOPTERA;

EID: 84954614356     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2336-6_3     Document Type: Article

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