GFP-based expression screening of membrane proteins in insect cells using the baculovirus system

Methods in Molecular Biology

Volumn 1261, Issue , 2015, Pages 197-209

  Hu, Nien Jen ^a   Rada, Heather ^b   Rahman, Nahid ^b   Nettleship, Joanne E ^b   Bird, Louise ^b   Iwata, So ^c   Drew, David ^d   Cameron, Alexander D ^e   Owens, Raymond J ^b  

^a NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

^b RUTHERFORD APPLETON LABORATORY   (United Kingdom)

^c IMPERIAL COLLEGE LONDON   (United Kingdom)

^d STOCKHOLM UNIVERSITY   (Sweden)

^e UNIVERSITY OF WARWICK   (United Kingdom)

Author keywords

Baculovirus; Fluorescencedetection size exclusion chromatography; Green fl uorescent protein; Insect cells; Membrane proteins

Indexed keywords

GREEN FLUORESCENT PROTEIN; MEMBRANE PROTEIN; HYBRID PROTEIN;

ANIMAL CELL; ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; FLUORESCENCE; FLUORESCENCE IMAGING; GEL PERMEATION CHROMATOGRAPHY; INSECT CELL; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN ISOLATION; ANIMAL; BACULOVIRIDAE; BIOSYNTHESIS; CYTOLOGY; GEL CHROMATOGRAPHY; GENE VECTOR; GENETICS; METABOLISM; SF9 CELL LINE; SPODOPTERA; VIROLOGY;

EUKARYOTA; HEXAPODA;

ANIMALS; BACULOVIRIDAE; CHROMATOGRAPHY, GEL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; MEMBRANE PROTEINS; RECOMBINANT FUSION PROTEINS; SF9 CELLS; SPODOPTERA;

EID: 84954604269     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2230-7_11     Document Type: Article

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