Improved expression of secreted and membrane-targeted proteins in insect cells

Biotechnology and Applied Biochemistry

Volumn 56, Issue 3, 2010, Pages 85-93

  Hitchman, Richard B ^a   Possee, Robert D ^a,b   Siaterli, Evangelia ^a   Richards, Kevin S ^c,g   Clayton, Amber J ^c,d   Bird, Louise E ^d   Owens, Raymond J ^d,h   Carpentier, David C J ^a,i   King, Fiona L ^e,j   Danquah, John O ^a,e   Spink, Karen G ^f   King, Linda A ^a,e  

^a Gypsy Lane Campus   (United Kingdom)

^b CENTRE FOR ECOLOGY AND HYDROLOGY   (United Kingdom)

^c NextGen Sciences Ltd   (United Kingdom)

^d UNIVERSITY OF OXFORD   (United Kingdom)

^e OXFORD BROOKES UNIVERSITY   (United Kingdom)

^f PFIZER GLOBAL RESEARCH AND DEVELOPMENT   (United Kingdom)

^g PUBLIC HEALTH ENGLAND   (United Kingdom)

^h RUTHERFORD APPLETON LABORATORY   (United Kingdom)

ⁱ IMPERIAL COLLEGE LONDON   (United Kingdom)

^j Tebu bio   (United Kingdom)

more..

Author keywords

Bacmid; Baculovirus; Cathepsin; Chitinase; Expression difficulty; High throughput

Indexed keywords

ENZYMES; THROUGHPUT; VIRUSES;

BACTERIAL CELLS; BACULOVIRUS; CELLULAR RESOURCES; CHITINASES; ENZYME ASSAYS; EXPRESSION LEVELS; GENE DELETION; HIGH THROUGHPUT; HIGH-LEVEL PRODUCTION; INSECT CELLS; MEMBRANE-BOUND PROTEINS; MULTIPLE PASSAGE; NUCLEAR PROTEINS; PROTEIN PRODUCTION; RECOMBINANT PROTEIN; RECOMBINANT PROTEIN EXPRESSION; RESTRICTION ENZYMES; SECRETORY PATHWAYS;

GENE EXPRESSION;

BACULOVIRUS VECTOR; CATHEPSIN; CHITINASE; RECOMBINANT PROTEIN; VIRUS DNA; MEMBRANE PROTEIN;

ARTICLE; BACTERIAL CELL; CONTROLLED STUDY; ENZYME ANALYSIS; ENZYME ASSAY; GENE DELETION; GENE INSERTION; INSECT CELL; MOLECULAR STABILITY; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; STABLE EXPRESSION; ANIMAL; BACULOVIRIDAE; BIOSYNTHESIS; CELL CULTURE; CYTOLOGY; ENZYMOLOGY; GENE EXPRESSION; GENETICS; HUMAN; INSECT; PROCEDURES; PROTEIN ENGINEERING;

BACTERIA (MICROORGANISMS); HEXAPODA;

ANIMALS; BACULOVIRIDAE; CATHEPSINS; CELLS, CULTURED; CHITINASE; GENE DELETION; GENE EXPRESSION; HUMANS; INSECTS; MEMBRANE PROTEINS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS;

EID: 77954833892     PISSN: 08854513     EISSN: None     Source Type: Journal    
DOI: 10.1042/BA20090130     Document Type: Article

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