Thymol and Carvacrol Prevent Doxorubicin-Induced Cardiotoxicity by Abrogation of Oxidative Stress, Inflammation, and Apoptosis in Rats

Journal of Biochemical and Molecular Toxicology

Volumn 30, Issue 1, 2016, Pages 37-44

  El Sayed, El Sayed M ^a   Mansour, Ahmed M ^a   Abdul Hameed, Mohammed S ^a  

^a Al Azhar University   (Egypt)

Author keywords

Anti Oxidant; Cardiotoxicity; Carvacrol; Doxorubicin; Thymol

Indexed keywords

ASPARTATE AMINOTRANSFERASE; CARVACROL; CASPASE 3; CATALASE; CREATINE KINASE; CREATINE KINASE MB; DOXORUBICIN; GLUTATHIONE; LACTATE DEHYDROGENASE; MALONALDEHYDE; SUPEROXIDE DISMUTASE; THYMOL; TROPONIN; TROPONIN I; TUMOR NECROSIS FACTOR ALPHA; CARDIOTONIC AGENT; TERPENE;

ADULT; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIOXIDANT ACTIVITY; APOPTOSIS; ARTICLE; ASPARTATE AMINOTRANSFERASE BLOOD LEVEL; CARDIOTOXICITY; COMPARATIVE STUDY; CONTROLLED STUDY; CREATINE KINASE BLOOD LEVEL; DRUG EFFECT; ENZYME ACTIVITY; HEART FUNCTION; HEART PROTECTION; HISTOPATHOLOGY; INFLAMMATION; LACTATE DEHYDROGENASE BLOOD LEVEL; MALE; NONHUMAN; OXIDATIVE STRESS; PROTEIN BLOOD LEVEL; RAT; SINGLE DRUG DOSE; TISSUE LEVEL; TREATMENT DURATION; ANIMAL; CARDIAC MUSCLE; DRUG EFFECTS; HEART; PATHOLOGY;

ANIMALS; APOPTOSIS; CARDIOTONIC AGENTS; CARDIOTOXICITY; DOXORUBICIN; HEART; INFLAMMATION; MALE; MONOTERPENES; MYOCARDIUM; OXIDATIVE STRESS; RATS; THYMOL;

EID: 84954026207     PISSN: 10956670     EISSN: 10990461     Source Type: Journal    
DOI: 10.1002/jbt.21740     Document Type: Article

References (51)

1. Booster DJ, Hortobagyi GN. Treatment of locally advanced breast cancer. Semin Oncol 1992;19(3):278-285.
2. Choi EH, Chang HJ, Cho JY, Chun HS. Cytoprotective effect of anthocyanins against doxorubicin-induced toxicity in H9c2 cardiomyocytes in relation to their antioxidant activities. Food Chem Toxicol 2007;45(10):1873-1881.
3. Steinherz LJ, Steinherz PG, Tan CT. Cardiac toxicity 4 to 20 years after completing anthracycline therapy. JAMA 1991;266(12):1672-1677.
4. Ewer MS, Jaffe N, Reid H, Zietz HA, Benjamin RS. Doxorubicin cardiotoxicity in children: comparison of consecutive divided daily dose administration schedule with single dose (rapid) infusion administration. Med Pediatr Oncol 1998;31(6):512-516.
5. Weiss RB. The anthracyclines: will we ever find a better doxorubicin? Semin Oncol 1992;19(6):670-686.
6. Octavia Y, Tocchetti CG, Gabrielson KL, Janssens S, Crijns HJ, Moens AL. Doxorubicin-induced cardiomyopathy: from molecular mechanisms to therapeutic strategies. J Mol Cell Cardiol 2012;52(6):1213-1225.
7. Wallace KB. Adriamycin-induced interference with cardiac mitochondrial calcium homeostasis. Cardiovasc Toxicol 2007;7(1):101-107.
8. Nakamura T, Ueda Y, Juan Y, Katsuda S, Takahashi H, Koh E. Fas-mediated apoptosis in adriamycin-induced cardiomyopathy in rats: in vivo study. Circulation 2000;102(5):572-578.
9. Nitobe J, Yamaguchi S, Okuyama M, Nozaki N, Sata M, Miyamoto T, Takeishi Y, Kubota I, Tomoike H. Reactive oxygen species regulate FLICE inhibitory protein (FLIP) and susceptibility to Fas-mediated apoptosis in cardiac myocytes. Cardiovasc Res 2003;57(1):119-128.
10. Niu J, Azfer A, Wang K, Wang X, Kolattukudy PE. Cardiac-targeted expression of soluble fas attenuates doxorubicin-induced cardiotoxicity in mice. J Pharmacol Exper Ther 2009;328(3):740-748.
11. Bast A, Haenen GR, Doelman CJ. Oxidants and anti oxidants: state of the alert. J Am Med 1991;91(3c):2S-13S.
12. Youdim KA, Joseph JA. A possible emerging role of phytochemicals in improving age-related neurological dysfunction: a multiplicity of effects. Free Radic Biol Med 2001;30(6):583-594
13. Akalin G, Incesu Z. The effects of carvacrol on apoptosis of H-Ras and N-Ras transformed cell lines. Turk J Pharm Sci 2011;8(2):105-116.
14. Jayakumar S, Madankumar A, Asokkumar S, Raghunandhakumar S, Gokuladhas K, Kamaraj S, Divya MG, Devaki T. Potential preventive effect of carvacrol against diethylnitrosamine-induced hepatocellular carcinoma in rats. Mol Cell Biochem 2012;360(1):51-60.
15. Ruberto G, Baratta MT, Deans SG, Dorman HJ. Antioxidant and antimicrobial activity of Foeniculum vulgare and Crithmum maritimum essential oils. Planta Med 2000;66(8):687-693.
16. Sokmen A, Sokmen M, Daferera D, Polissiou M, Candan F, Unlu M, Akpulat HA. The in vitro antioxidant and antimicrobial activities of the essential oil and methanol extracts of Achillea biebersteinii Afan (Asteraceae). Phytother Res 2004;18(6):451-456.
17. Zeidan-Chulía F, Gursoy M, deOliveira BH, Gelain DP, Kononen E, Gursoy UK, Moreira JC, Uitto VJ. Focused micro array analysis of apoptosis in periodontitis and its potential pharmacological targeting by CAR. Arch Oral Biol 2014;59(5):461-469.
18. Aristatile B, Al-Assaf AH, Pugalendi KV. Carvacrol suppresses the expression of inflammatory marker genes in D-galactosamine-hepatotoxic rats. Asian Pac J Trop Med 2013;6(3):205-211.
19. Tsai ML, Lin CC, Lin WC, Yang CH. Antimicrobial, antioxidant and anti-inflammatory activities of essential oils from five selected herbs. Biosci Biotechnol Biochem 2011;75(10):1977-1983.
20. Alam K, Nagi MN, Badary OA, Al-Shabanah OA, Al-Rikabi AC, Al-Bekairi AM. The protective action of thymol against carbon tetrachloride hepatotoxicity in mice. Pharmacol Res 1999;40(2):159-163.
21. Liang D, Li F, Fu Y, Cao Y, Song X, Wang T, Wang W, Guo M, Zhou E, Li D, Yang Z, Zhang N. Thymol inhibits LPS stimulated inflammatory response via down-regulation of NF-κ B and MAPK signaling pathways in mouse mammary epithelial cells. Inflammation 2014;37(1):214-222.
22. Milos M, Makota D. Investigation of antioxidant synergisms and antagonisms among thymol, CAR, thymoquinone and p-cymene in a model system using the Briggs-Rauscher oscillating reaction. Food Chem 2012;131(1):296-299.
23. Nazmi AS, Ahmad SJ, Rashikh A, Akhtar M, Pillai KK, Najmi AK. Protective effects of 'Khamira Abresham Hakim Arshad Wala', a unani formulation against doxorubicin-induced cardiotoxicity and nephrotoxicity. Toxicol Mech Methods 2011;21(1):41-47.
24. El-Sayed EM, Abdallah AR, Mansour AM, El-Araby AA. Thymol and carvacrol prevent cisplatin-induced nephrotoxicity by abrogation of oxidative stress, inflammation, and apoptosis in rats. J Biochem Mol Toxicol 2014;29(4):165-172.
25. Yu W, Liu Q, Zhu S. Carvacrol protects against acute myocardial infarction of rats via anti-oxidative and anti-apoptotic pathways. Biol Pharm Bull 2013;36(4):579-584.
26. Institute of Laboratory Animal Resources. Guide for the care and use of laboratory animals, 8th edition. Washington, DC: National Academies Press; 1996.
27. Doroshow and Davies. Redox cycling of anthracyclines by cardiac mitochondria. II. Formation of superoxide anion, hydrogen peroxide, and hydroxyl radical. J Biol Chem 1986;261(7):3068-3074.
28. Doroshow JH, Locker GY, Myers CE. Enzymatic defenses of the mouse heart against reactive oxygen metabolites: alterations produced by doxorubicin. J Clin Invest 1980;65(1):128-135.
29. Takemura G, Fujiwara H. Doxorubicin-induced cardiomyopathy from the cardiotoxic mechanisms to management. Prog Cardiovasc Dis 2007;49(5):330-352.
30. Swain SM, Whaley FS, Ewer MS. Congestive heart failure in patients treated with doxorubicin. A retrospective analysis of three trials. Cancer 2003;97(11):2869-2879.
31. Khan G, Haque ES, Anwer T, Ahsan MN, Safhi MM, Alam MF. Cardioprotective effect of green tea extract on doxorubicin induced- cardiotoxicity in rats. Acta Pol Pharm 2014;71(5):861-868.
32. Ashour OM, Elberry AA, Alahdal AM, Al Mohamadi AM, Nagy AA, Abdel-Naim AB, Abdel-Sattar EA, Mohamadin AM. Protective effect of bilberry (Vaccinium myrtillus) against doxorubicin-induced oxidative cardiotoxicity in rats. Med Sci Monit 2011;17(4):110-115.
33. Elberry AA, Abdel-Naim AB, Abdel-Sattar EA, Nagy AA, Mosli HA, Mohamadin AM, Ashour OM. Cranberry (Vaccinium macrocarpon) protects against doxorubicin-induced cardiotoxicity in rats. Food Chem Toxicol 2010;48(5):1178-1184.
34. Lowry OH, Rosehmugh NJ, Farr AL, Randall KJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193(1):265-275.
35. Mihara M, Uchiyama M. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem 1978; 86(1):271-278
36. Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1959;82(1):70-77.
37. Clariborne A. Catalase activity. In: Greenwold RA, editor. Handbook of methods for oxygen radical research. Boca Raton, FL: CRC Press; 1985. pp 283-284.
38. Marklund SL. Product of extracellular-superoxide dismutase catalysis. FEBS Lett 1985;184:237-239.
39. Banchroft JD, Stevens A, Turner DR. Theory and practice of histological techniques, 4th edition. New York: Churchill Livingstone 1996.
40. Shan K, Lincoff AM, Young JB. Anthracycline-induced cardiotoxicity. Ann Intern Med 1996;125(1):47-58
41. Hortobagyi GN. Anthracyclines in the treatment of cancer. An overview. Drugs 1997;54(4):1-7
42. Nazeyrollas P, Prévost A, Baccard N, Manot L, Devillier P, Millart H: Effects of amifostine on perfused isolated rat heart and on acute doxorubicin-induced cardiotoxicity. Cancer Chemother Pharmacol 1999;43(3):227-232.
43. Liu X, Chen Z, Chua CC, Ma Y, Youngberg GA, Hamdy R, Chua BHL. Melatonin as an effective protector against doxorubicin-induced cardiotoxicity. Am J Physiol Heart Circ Physiol 2002;283(1):H254-H263.
44. Parker MA, King V, Howard KP. Nuclear magnetic resonance study of doxorubicin binding to cardiolipin containing magnetically oriented phospholipid bilayers. Biochim Biophys Acta 2001;1514(2):206-216.
45. Tokarska-Schlattner M, Zaugg M, da Silva R, Lucchinetti E, Schaub MC, Wallimann T, Schlattner U. Acute toxicity of doxorubicin on isolated perfused heart. Response of kinases regulating energy supply. Am J Physiol Heart Circ Physiol 2005;289(1):H37-H47.
46. Hadi N, Yousif NG, Al-Amran FG, Huntei NK, Mohammed BI, Ali SJ. Vitamin E and telmisartan attenuates doxorubicin-induced cardiac injury in rat through down regulation of inflammatory response. BMC Cardiovasc Disord 2012;12:63.
47. Wang S, Konorev EA, Kotamraju S, Joseph J, Kalivendi S, Kalyanaraman B. Doxorubicin induces apoptosis in normal and tumor cells via distinctly different mechanisms: intermediacy of H2O2 and p53 dependent pathways. J Biol Chem 2004;279(24):25535-25543.
48. Wang H, Yu P, Gou H, Zhang J, Zhu M, Wang Z, Tian J, Jiang Y, Fu F. Cardioprotective effects of 20(S)-ginsenoside Rh2 against doxorubicin-induced cardiotoxicity in vitro and in vivo. Evid Based Complement Alternat Med 2012;2012:506214.
49. Song X, Chen A, Liu Y, Wang XB, Zhou Y, Liu L, Zhang X, Wang L, Yang P. CAR pretreatment attenuates myocardial oxidative stress and apoptosis following myocardial ischemia-reperfusion in mice. Nan Fang Yi Ke Da Xue Xue Bao 2013;33(11):1624-1627.
50. Wu Y, Luo Y, Wang Q. Antioxidant and antimicrobial properties of essential oils encapsulated in zein nanoparticles prepared by liquid-liquid dispersion method. LWT-Food Sci Technol 2012;48(2):283-290.
51. Yanishlieva NV, Marinova EM, Gordon MH, Raneva VG. Antioxidant activity and mechanism of action of thymol and carvacrol in two lipid systems. Food Chem 1999;64(1):59-66.