Total phenolic content, antioxidant activity and pre-clinical safety evaluation of an Anacardium occidentale stem bark Portuguese hypoglycemic traditional herbal preparation

Industrial Crops and Products

Volumn 82, Issue , 2016, Pages 171-178

  Encarnação, Sofia ^a   de Mello Sampayo, Cristina ^a   Graça, Nuno A G ^a   Catarino, Luís ^b,c   da Silva, Isabel B Moreira ^a   Lima, Beatriz Silva ^a   Silva, Olga Maria Duarte ^a  

^a RESEARCH INSTITUTE FOR MEDICINES IMED ULISBOA   (Portugal)

^b UNIVERSITY OF LISBON   (Portugal)

^c CENTRO DE INVESTIGAÇÃO EM BIODIVERSIDADE E RECURSOS GENÉTICOS   (Portugal)

Author keywords

Cashew tree bark; Genotoxicity; Herbal medicines; Plant raw material; Repeated dose toxicity; Total phenolic content

Indexed keywords

ANTIOXIDANTS; FORESTRY; FREE RADICALS; MAMMALS; TOXICITY;

ANTI-OXIDANT ACTIVITIES; CONCENTRATION-DEPENDENT; FREE RADICAL SCAVENGING ACTIVITY; GENOTOXICITIES; HERBAL MEDICINES; RADICAL SCAVENGING ACTIVITY; TOTAL PHENOLIC CONTENT; TREE BARKS;

PLANTS (BOTANY);

ANTIOXIDANT; BARK; FREE RADICAL; GENOTOXICITY; HERB; MEDICINE; PHENOL; SCAVENGING (CHEMISTRY); STEM;

ANACARDIUM OCCIDENTALE; MUS;

EID: 84952980405     PISSN: 09266690     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.indcrop.2015.11.001     Document Type: Article

References (63)

1. Aherne S., Kerry J., O'Brien N. Effects of plant extracts on antioxidant status and oxidant-induced stress in Caco-2 cells. Br. J. Nutr. 2007, 97:321-328.
2. Alexander-Lindo R., Morrison E., Nair M., McGrowder D. Effect of the fractions of the hexane bark extract and stigmast-4-en-3-one isolated from Anacardium occidentale on blood glucose tolerance test in an animal model. Int. J. Pharm. 2007, 3:41-47.
3. Arekemase M.O., Oyeyiola G.P., Aliyu M.B. Antibacterial activity of Anacardium occidentale on some enterotoxin producing bacteria. Int. J. Biol. 2011, 3:92-99.
4. Barcelos G., Shimabukuro F., Maciel M., Cólus I. Genotoxicity and antigenotoxicity of cashew (Anacardium occidentale L.) in V79 cells. Toxicol. In Vitro 2007, 21:1468-1475.
5. Barcelos G., Shimabukuro F., Mori M., Maciel M., Cólus I. Evaluation of mutagenicity and antimutagenicity of cashew stem bark methanolic extract in vitro. J. Ethnopharmacol. 2007, 114:268-273.
6. Bassey T., Eliakim-Ikechukwu C., Ihentuge C. Effect of ethanolic stem-bark extract of Anacardium occidentale (cashew) on the histology of the pancreas of diabetic wistar rats. JEBAS 2012, 2:153-156.
7. Brandao M.G.L., Zanetti N.N.S., Oliveira P., Grael C.F.F., Santos A.C.P., Monte-Mor R.L.M. Brazilian medicinal plants described by 19th century European naturalists and in the Official Pharmacopoeia. J. Ethnopharmacol. 2008, 120:141-148.
8. Cano J.H., Volpato G. Herbal mixtures in the traditional medicine of Eastern Cuba. J. Ethnopharmacol. 2004, 90:293-316.
9. Carvalho A., Annoni R., Silva P., Borelli P., Fock R., Trevisan M., Mauad T. Acute, subacute toxicity and mutagenic effects of anacardic acids from cashew (Anacardium occidentale Linn.) in mice. J. Ethnopharmacol. 2011, 135:130-136.
10. Chabi Sika K., Adoukonou-Sagbadja H., Ahoton L.E., Adebo I., Adigoun F.A., Saidou A., Kotchoni S.O., Ahanchede A., Baba-Moussa L. Indigenous knowledge and traditional management of cashew (Anacardium occidentale L.) genetic resources in Benin. JEBAS 2013, 1:375-382.
11. Chaves M., Citó A., Lopes J., Costa D., Oliveira C., Costa A., Brito Júnior F. Total phenolics, antioxidant activity and chemical constituents from extracts of Anacardium occidentale L. Anacardiaceae. Braz. J. Pharmacogn. 2010, 20:106-112.
12. Collins A. The comet assay for DNA damage and repair-principles, applications, and limitations. Mol. Biotechnol. 2004, 26:249-261.
13. Comissão da Farmacopeia Portuguesa Farmacopeia Portuguesa 2009, INFARMED, Lisboa. 9a edição.
14. EMA Guideline on Repeated Dose Toxicity 2010, European Medicines Agency, London.
15. Eliakim-Ikechukwu C., Obri A., Akpa O. Phytochemical and micronutrient composition of Anacardium occidentale Linn. (cashew) stem-bark hydroethanolic extract and its effect on the fasting blood glucose levels and body weight of diabetic wistar rats. Internet J. Nutr. Wellness 2010, 10.
16. Encarnação S., Malmir M., Sousa D., Silva I.M., Mello-Sampayo C., Serrano R., Lima B., Silva O. Phenol content, antioxidant and α- and β-glucosidase inhibitory activities of an Anacardium occidentale stem bark Traditional Herbal Preparation. Planta Med. 2014, 80:P2B42.
17. FAO, 1997. [WWW document]. Medicinal plants for forest conservation and health care. Food and Agriculture Organization of the United Nations. Available: (accessed 01.06.15.).
18. FAO, 2013. [WWW document]. Cashew nuts, with shell. Food and Agriculture Organization of the United Nations Statistics Division. Available: (accessed 15.02.15.).
19. França F., Cuba C.A.C., Moreira E.A., Miguel O., Almeida M., Virgens M.L., Marsden P.D. Avaliação do efeito do extrato de casca de cajueiro-branco (Anacardium occidentale L.) sobre a infecção por Leishmania (Viannia) brasiliensis. Rev. Soc. Bras. Med. Trop. 1993, 26:151-155.
20. George P. Concerns regarding the safety and toxicity of medicinal plants-an overview. JAPS 2011, 1:40-44.
21. Hayashi M. In vivo rodent micronucleus assay. Chromosomal Alterations: Methods, Results and Importance in Human Health 2007, 257-270. Springer-Verlag, Berlin, Heidelberg. G. Obe, Vijayalaxmi (Eds.).
22. ICH International Conference on Harmonisation Guideline S2 (R1): Guidance for Industry. S2 (R1) Genotoxicity Testing and Data Interpretation for Pharmaceuticals Intended for Human Use 2012, U.S. Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research, Center for Biologics Evaluation and Research, Rockville.
23. Iwu M.M. Handbook of African Medicinal Plants 2014, CRC Press, Boca Raton. 2nd ed.
24. Iyawe H.O.T., Azih M.C. Total phenolic contents and lipid peroxidation potentials of some tropical antimalarial plants. Eur. J. Med. Plants 2011, 1:33-39.
25. Jordan S.A., Cunningham D.G., Marles R.J. Assessment of herbal medicinal products: challenges, and opportunities to increase the knowledge base for safety assessment. Toxicol. Appl. Pharmacol. 2010, 243:198-216.
26. Kimura A., Miyata A., Honma M. A combination of in vitro comet assay and micronucleus test using human lymphoblastoid TK6 cells. Mutagenesis 2013, 28:583-590.
27. Lahlou M. The success of natural products in drug discovery. J. Pharm. Pharmacol. 2013, 4:17-31.
28. Liao W., McNutt M., Zhu W. The comet assay: a sensitive method for detecting DNA damage in individual cells. Methods 2009, 48:46-53.
29. Ling L. Hypoglycemic and antioxidative effects of Anacardium occidentale Linn. in diabetic rats. Masters Thesis 2006, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia.
30. Lutz M.B., Kukutsch N., Ogilvie A.L., Rößner S., Koch F., Romani N., Schuler G. An advanced culture method for generating large quantities of highly pure dendritic cells from mouse bone marrow. J. Immunol. Methods 1999, 223:77-92.
31. Lynch A., Sasaki J., Elespuru R., Jacobson-Kram D., Thybaud V., De Boeck M., Aardema M., Aubrecht J., Benz D., Dertinger S., Douglas G., White P., Escobar P., Fornace Jr A., Honma M., Naven R., Rusling J., Schiestl R., Walmsley R., Yamamura E., van Benthem J., Kim J. New and emerging technologies for genetic toxicity testing. Environ. Mol. Mutagen. 2011, 52:205-223.
32. Manoharan K., Banerjee M.R. Beta-carotene reduces sister chromatid exchange induced chemical carcinogens in mouse mammary cells in organ culture. Cell Biol. Int. Rep. 1985, 9:783-789.
33. Melo A., Diniz M., Santos H., Silva M., Medeiros I. Acute pre-clinical toxicological assays of the raw extract of the bark of Anacardium occidentale L. on mice. Rev. Bras. Ciênc. Saúde. 2004, 8:217-222.
34. Melo A., Albuquerque M., Silva M., Gomes G., Cruz I., Leite V., Higino J. Evaluation of the subchronic toxicity of the crude dry extract of Anacardium occidentale Linn. in dogs. Acta Sci. Health Sci. 2006, 28:37-41.
35. Mitra R., Mitchell B., Gray C., Orbell J., Coulepis T., Muralitharan M. Medicinal plants of Brazil. Asia Pac. Biotech News 2007, 11:689-706.
36. Mota M.L., Thomas G., Barbosa Filho J.M. Anti-inflammatory actions of tannins isolated from the bark of Anacardium occidentale L. J. Ethnopharmacol. 1985, 13:289-300.
37. OECD OECD Guideline 474-Guideline for the Testing of Chemicals: Mammalian Erythrocyte Micronucleus Test 1997, Organisation for Economic Co-operation and Development, Paris.
38. Ofusori D.A., Enaibe B.U., Adelakun A.E., Adesanya A.O., Ude R.A., Oluyemi K.A., Okwuonu C.U., Apantaku O.A. Microstructural study of the effect of ethanolic extract of cashew stem bark Anacardium occidentale on the brain and kidney of Swiss albino mice. Internet J. Altern. Med. 2008, 5:3.
39. Ojewole J.A.O. Laboratory evaluation of the hypoglycemic effect of Anacardium occidentale Linn. (Anacardiaceae) stem-bark extracts in rats. Methods Find. Exp. Clin. 2003, 25:199-204.
40. Ojezele M.O., Agunbiade S. Phytochemical constituents and medicinal properties of different extracts of Anacardium occidentale and Psidium guajava. AJBPS 2013, 3:20-23.
41. Okonkwo T.J., Okorie O., Okonta J.M., Okonkwo C.J. Sub-chronic hepatotoxicity of Anacardium occidentale (Anacardiaceae) inner stem bark extract in rats. Indian J. Pharm. Sci. 2010, 72:353-357.
42. Olajide O., Aderogba M., Adedapo A., Makinde J. Effects of Anacardium occidentale stem bark extract on in vivo inflammatory models. J. Ethnopharmacol. 2004, 95:139-142.
43. Olatunji L.A., Okwusidi J.I., Soladoye A.O. Antidiabetic effect of Anacardium occidentale stem-bark in fructose-diabetic rats. Pharm. Biol. 2005, 43:589-593.
44. Oliveira M., Lemos L., Oliveira R., Dall'Oglio E., Sousa Júnior P., Martins D. Evaluation of toxicity of Calophyllum brasiliense stem bark extract by in vivo and in vitro assays. J. Ethnopharmacol. 2014, 155:30-38.
45. Petrovska B. Historical review of medicinal plants usage. Pharmacogn. Rev. 2012, 6:1-5.
46. Recio L., Hobbs C., Caspary W., Witt K. Dose-response assessment of four genotoxic chemicals in a combined mouse and rat micronucleus and comet assay protocol. J. Toxicol. Sci. 2010, 35:149-162.
47. Rojas E., Lopez M., Valverde M. Single cell gel electrophoresis assay: methodology and applications. J. Chromatogr. B 1999, 722:225-254.
48. Salim A.A., Chin Y.-W., Kinghorn A.D. Drug discovery from plants. Bioactive Molecules and Medicinal Plants 2008, 1-24. Springer-Verlag, Berlin, Heidelberg, (Chapter 1). K.G. Ramawat, J.M. Mérillon (Eds.).
49. Scalbert A., Monties B., Janin G. Tannins in wood-comparison of different estimation methods. J. Agric. Food Chem. 1989, 37:1324-1329.
50. Sha'a K., Ajayi O., Arong G. The in vitro antimalarial activity of aqueous and ethanolic extracts of Anacardium occidentale against Plasmodium falciparum in Damboa, North-Eastern Nigeria. IJST 2014, 4:80-85.
51. Silva T.M.S., Camara C.A., Lins A.C.S., Barbosa Filho J.M., Silva E.M., Freitas B.M., Santos F.A.R. Chemical composition and free radical scavenging activity of pollen loads from stingless bee Melipona subnitida Ducke. J. Food Compos. Anal. 2006, 19:507-511.
52. Silva O. Caderno de Campo Plantas Medicinais Usadas em Portugal para o Tratamento da Diabetes 2000, Faculty of Pharmacy, Universidade de Lisboa, (unpublished).
53. Singh N., McCoy M., Tice R., Schneider E. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res. 1988, 175:184-191.
54. Sokeng S.D., Lontsi D., Moundipa P.F., Jatsa H.B., Watcho P., Kamtchouing P. Hypoglycemic Effect of Anacardium occidentale L. Methanol extract and fractions on streptozotocin-induced diabetic rats. Global J. Pharmacol. 2007, 1:1-5.
55. Tédong L., Dzeufiet P., Dimo T., Asongalem E., Sokeng S., Flejou J., Callard P., Kamtchouing P. Acute and subchronic toxicity of Anacardium occidentale Linn. (Anacardiaceae) leaves hexane extract in mice. Afr. J. Tradit. Complement. Altern. Med. 2007, 4:140-147.
56. Tchikaya F., Bantsielé G., Kouakou-Siransy G., Datté J., Yapo P., Zirihi N., Offoumou M. Anacardium occidentale Linn. (Anacardiaceae) stem bark extract induces hypotensive and cardio-inhibitory effects in experimental animal models. Afr. J. Tradit. Complement. Altern. Med. 2011, 8:452-461.
57. Thirugnanasampandan R., Jayakumar J. Protection of cadmium chloride induced DNA damage by Lamiaceae plants. Asian Pac. J. Trop. Biomed. 2011, 1:391-394.
58. Tice R., Agurell E., Anderson D., Burlinson B., Hartmann A., Kobayashi H., Miyamae Y., Rojas E., Ryu J., Sasaki Y. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ. Mol. Mutagen. 2000, 35:206-221.
59. U.S.FDA Guidance for Industry-Estimating the Maximum Safe starting Dose in Initial Clinical Trials for Therapeutics in Adult Healthy Volunteers 2005, U.S. Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research, Rockville.
60. Vanderlinde F., Landim H., Galdino H., Maciel M., Anjos G., Malvar D., Côrtes W., Rocha F. Evaluation of the antinociceptive and anti-inflammatory effects of the acetone extract from Anacardium occidentale L. Braz. J. Pharm. Sci. 2009, 45:437-442.
61. WHO WHO Guidelines on Safety Monitoring of Herbal Medicines in Pharmacovigilance Systems 2004, World Health Organization, Geneva.
62. Yahia E. Postharvest Biology and Technology of Tropical and Subtropical Fruits 2011, vol. 2. Woodhead Publishing, Cambridge.
63. Yusuf S., Aliyu M., Ndanusa R. Effect of aqueous extract of Anacardium occidentale L. stem bark on sodium and chloride transport in the rabbit colon. J. Med. Plants Res. 2009, 6:493-497.