Droplet digital PCR for absolute quantification of EML4-ALK gene rearrangement in lung adenocarcinoma

Journal of Molecular Diagnostics

Volumn 17, Issue 5, 2015, Pages 515-520

  Wang, Qiushi ^a   Yang, Xin ^a   He, Yong ^a   Ma, Qiang ^a   Lin, Li ^a   Fu, Ping ^a   Xiao, Hualiang ^a  

^a THIRD MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CRIZOTINIB; FORMALDEHYDE; PARAFFIN; ANTINEOPLASTIC AGENT; EML4-ALK FUSION PROTEIN, HUMAN; ONCOPROTEIN; PYRAZOLE DERIVATIVE; PYRIDINE DERIVATIVE;

ADULT; AGED; ARTICLE; CANCER CHEMOTHERAPY; DRUG EFFICACY; FEMALE; FLUORESCENCE IN SITU HYBRIDIZATION; GENE DOSAGE; GENE REARRANGEMENT; GENETIC VARIABILITY; HUMAN; HUMAN TISSUE; LUNG ADENOCARCINOMA; MAJOR CLINICAL STUDY; MALE; POLYMERASE CHAIN REACTION; ADENOCARCINOMA; GENE TRANSLOCATION; GENETICS; LUNG NEOPLASMS; MIDDLE AGED; PROCEDURES; PROGNOSIS; TREATMENT OUTCOME;

ADENOCARCINOMA; ADULT; AGED; ANTINEOPLASTIC AGENTS; FEMALE; GENE DOSAGE; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; LUNG NEOPLASMS; MALE; MIDDLE AGED; ONCOGENE PROTEINS, FUSION; POLYMERASE CHAIN REACTION; PROGNOSIS; PYRAZOLES; PYRIDINES; TRANSLOCATION, GENETIC; TREATMENT OUTCOME;

EID: 84952656127     PISSN: 15251578     EISSN: 19437811     Source Type: Journal    
DOI: 10.1016/j.jmoldx.2015.04.002     Document Type: Article

References (19)

1. M. Laffert, A. Warth, R. Penzel, P. Schirmacher, D. Jonigk, H. Kreipe, H.U. Schildhaus, S. Merkelbach-Bruse, R. Büttner, S. Reu, R. Kerler, A. Jung, T. Kirchner, C. Wölfel, I. Petersen, R. Rodriguez, W. Jochum, H. Bartsch, A. Fisseler-Eckhoff, E. Berg, D. Lenze, M. Dietel, and M. Hummel Anaplastic lymphoma kinase (ALK) gene rearrangement in non-small cell lung cancer (NSCLC): results of a multi-centre ALK-testing Lung Cancer 81 2013 200 206
2. M. Soda, Y.L. Choi, M. Enomoto, S. Takada, Y. Yamashita, S. Ishikawa, S. Fujiwara, H. Watanabe, K. Kurashina, H. Hatanaka, M. Bando, S. Ohno, Y. Ishikawa, H. Aburatani, T. Niki, Y. Sohara, Y. Sugiyama, and H. Mano Identification of the transforming EML4-ALK fusion gene in non-small-cell lung cancer Nature 448 2007 561 566
3. A.T. Shaw, D.W. Kim, K. Nakagawa, T. Seto, L. Crinó, M.J. Ahn, T. De Pas, B. Besse, B.J. Solomon, F. Blackhall, Y.L. Wu, M. Thomas, K.J. O'Byrne, D. Moro-Sibilot, D.R. Camidge, T. Mok, V. Hirsh, G.J. Riely, S. Iyer, V. Tassell, A. Polli, K.D. Wilner, and P.A. Jänne Crizotinib versus chemotherapy in advanced ALK-positive lung cancer N Engl J Med 368 2013 2385 2394
4. Y. Li, Y. Pan, R. Wang, Y. Sun, H. Hu, X. Shen, Y. Lu, L. Shen, X. Zhu, and H. Chen ALK-rearranged lung cancer in Chinese: a comprehensive assessment of clinicopathology, IHC, FISH and RT-PCR PLoS One 8 2013 e69016
5. P. Martinez, J. Hernández-Losa, M.Á. Montero, S. Cedrés, J. Castellví, A. Martinez-Marti, N. Tallada, N. Murtra-Garrell, A. Navarro-Mendivill, V. Rodriguez-Freixinos, M. Canela, S. Ramon y Cajal, and E. Felip Fluorescence in situ hybridization and immunohistochemistry as diagnostic methods for ALK positive non-small cell lung cancer patients PLoS One 8 2013 e52261
6. Y.G. Zhang, M.L. Jin, L. Li, H.Y. Zhao, X. Zeng, L. Jiang, P. Wei, X.L. Diao, X. Li, Q. Cao, and X.X. Tian Evaluation of ALK rearrangement in Chinese non-small cell lung cancer using FISH, immunohistochemistry, and real-time quantitative RT- PCR on paraffin-embedded tissues PLoS One 8 2013 e64821
7. Q. Zhou, X.C. Zhang, Z.H. Chen, X.L. Yin, J.J. Yang, C.R. Xu, H.H. Yan, H.J. Chen, J. Su, W.Z. Zhong, X.N. Yang, S.J. An, B.C. Wang, Y.S. Huang, Z. Wang, and Y.L. Wu Relative abundance of EGFR mutations predicts benefit from gefitinib treatment for advanced non-small-cell lung cancer J Clin Oncol 9 2011 3316 3321
8. A. Bharuthram, M. Paximadis, A.C.P. Picton, and C.T. Tiemessen Comparison of a quantitative real-time PCR assay and droplet digital PCR for copy number analysis of the CCL4L genes Infect Genet Evol 25 2014 28 35
9. P. Belgrader, S.C. Tanner, J.F. Regan, R. Koehler, B.J. Hindson, and A.S. Brown Droplet digital PCR measurement of HER2 copy number alteration in formalin-fixed paraffin-embedded breast carcinoma tissue Clin Chem 59 2013 991 994
10. K. Tuononen, V.K. Sarhadi, A. Wirtanen, M. Rönty, K. Salmenkivi, A. Knuuttila, S. Remes, A.I. Telaranta-Keerie, S. Bloor, P. Ellonen, and S. Knuutila Targeted resequencing reveals ALK fusions in non-small cell lung carcinomas detected by FISH, immunohistochemistry, and real-time RT-PCR: a comparison of four methods Biomed Res Int 2013 2013 757490
11. B.J. Hindson, K.D. Ness, D.A. Masquelier, P. Belgrader, N.J. Heredia, A.J. Makarewicz, and et al. High-throughput droplet digital PCR system for absolute quantitation of DNA copy number Anal Chem 15 2011 8604 8610
12. N.J. Heredia, P. Belgrader, S. Wang, R. Koehler, J. Regan, A.M. Cosman, S. Saxonov, B. Hindson, S.C. Tanner, A.S. Brown, and G. Karlin-Neumann Droplet Digital™ PCR quantitation of HER2 expression in FFPE breast tissue samples Methods 59 2013 183 186
13. A.T. Shaw, B.Y. Yeap, B.J. Solomon, G.J. Riely, J. Gainor, J.A. Engelman, G.I. Shapiro, D.B. Costa, S.H. Ou, M. Butaney, R. Salgia, R.G. Maki, M. Varella-Garcia, R.C. Doebele, Y.J. Bang, K. Kulig, P. Selaru, Y. Tang, K.D. Wilner, E.L. Kwak, J.W. Clark, A.J. Iafrate, and D.R. Camidge Effect of crizotinib on overall survival in patients with advanced non-small cell lung cancer harbouring ALK gene rearrangement: a retrospective analysis Lancet Oncol 12 2011 1004 1012
14. M.L. Wallander, K.B. Geiersbach, S.R. Tripp, and L.J. Layfield Comparison of reverse transcription-polymerase chain reaction, immunohistochemistry, and fluorescence in situ hybridization methodologies for detection of echinoderm microtubule-associated proteinlike 4-anaplastic lymphoma kinase fusion-positive non-small cell lung carcinoma: implications for optimal clinical testing Arch Pathol Lab Med 136 2012 796 803
15. A.S. Whale, J.F. Huggett, S. Cowen, V. Speirs, J. Shaw, S. Ellison, C.A. Foy, and D.J. Scott Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation Nucleic Acids Res 40 2012 e82
16. T. Hoshino, and F. Inagaki Molecular quantification of environmental DNA using microfluidics and digital PCR Syst Appl Microbiol 35 2012 390 395
17. J.F. Huggett, and A. Whale Digital PCR as a novel technology and its potential implications for molecular diagnostics Clin Chem 59 2013 1691 1693
18. Q. Zhou, X.C. Zhang, Z.H. Chen, X.L. Yin, J.J. Yang, C.R. Xu, H.H. Yan, H.J. Chen, J. Su, W.Z. Zhong, X.N. Yang, S.J. An, B.C. Wang, Y.S. Huang, Z. Wang, and Y.L. Wu Relative abundance of EGFR mutations predicts benefit from gefitinib treatment for advanced non-small-cell lung cancer J Clin Oncol 29 2011 3316 3321
19. E. Thunnissen, L. Bubendorf, M. Dietel, G. Elmberger, K. Kerr, F. Lopez-Rios, H. Moch, W. Olszewski, P. Pauwels, F. Penault-Llorca, and G. Rossi EML4-ALK testing in non-small cell carcinomas of the lung: a review with recommendations Virchows Arch 461 2012 245 257