Droplet digital PCR measurement of HER2 copy number alteration in formalin-fixed paraffin-embedded breast carcinoma tissue

Clinical Chemistry

Volumn 59, Issue 6, 2013, Pages 991-994

  Belgrader, Phillip ^a   Tanner, Stephanie C ^b   Regan, John F ^a   Koehler, Ryan ^a   Hindson, Benjamin J ^a   Brown, Alexandra S ^b  

^a BIO RAD LABORATORIES   (United States)

^b UNIVERSITY OF MISSISSIPPI MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EPIDERMAL GROWTH FACTOR RECEPTOR 2; FORMALDEHYDE; PARAFFIN;

ACCURACY; ARTICLE; BREAST CARCINOMA; CANCER TISSUE; CENTROMERE; CHROMOSOME 17; CONTROLLED STUDY; COPY NUMBER VARIATION; DNA TEMPLATE; DROPLET DIGITAL POLYMERASE CHAIN REACTION; FLUORESCENCE IN SITU HYBRIDIZATION; GENE AMPLIFICATION; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; INVASIVE CARCINOMA; MOLECULAR PROBE; PARTICLE SIZE; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS;

BREAST NEOPLASMS; COMPUTERS; FEMALE; FORMALDEHYDE; GENE DOSAGE; GENETIC TECHNIQUES; HUMANS; PARAFFIN EMBEDDING; POLYMERASE CHAIN REACTION; RECEPTOR, ERBB-2; REPRODUCIBILITY OF RESULTS;

AVES;

EID: 84878468245     PISSN: 00099147     EISSN: 15308561     Source Type: Journal    
DOI: 10.1373/clinchem.2012.197855     Document Type: Article

References (19)

1. Howlader N, Noone AM, Krapcho M, Neyman N, Aminou R, Waldron W, et al., (eds). SEER Cancer Statistics Review, 1975-2008, National Cancer Institute. Bethesda, MD. Based on November 2010 SEER data submission, posted to the SEER website, 2011. http://seer.cancer.gov/csr/1975-2008/ (Accessed January 2013).
2. Moelans CB, de Weger RA, Van der Wall E, van Diest PJ. Current technologies for HER2 testing in breast cancer. Crit Rev Oncol Hematol 2011;80:380-92.
3. Vogel C, Cobleigh MA, Tripathy D, Gutheil JC, Harris LN, Fehrenbacher L, et al. First-line, single-agent Herceptin® (trastuzumab) in metastatic breast cancer: a preliminary report. Eur J Cancer 2001;37:25-9.
4. Partho P, Sengupta DW, Northfelt FG, Jose LZ, Bijoy KK. Trastuzumab-induced cardiotoxicity: heart failure at the crossroads. Mayo Clin Proc 2008;83:197-203.
5. Wolff AC, Hammond ME, Schwartz JN, Hagerty KL, Allred DC, Cote RJ, et al. American Society of Clinical Oncology/College of American Pathologists guideline recommendations for human epidermal growth factor receptor 2 testing in breast cancer. J Clin Oncol 2007;25:118-45. (Pubitemid 350003059)
6. Gjerdrum LM, Sorensen BS, Kjeldsen E, Sorensen FB, Nexo E, Hamilton-Dutoit S. Real-time quantitative PCR of microdissected paraffin-embedded breast carcinoma: an alternative method for HER-2/neu analysis. J Mol Diagn 2004;6:42-51. (Pubitemid 38208344)
7. Lyon E, Millson A, Lowery MC, Woods R, Wittwer CT. Quantification of HER2/neu gene amplification by competitive PCR using fluorescent melting curve analysis. Clin Chem 2001;47:844-5.
8. Dabbs DD, Klein ME, Mohsin SK, Tubbs RR, Shuai Y, Bhargava R. High false-negative rate of HER2 quantitative reverse transcription polymerase chain reaction of the Oncotype DX test: an independent quality assurance study. J Clin Oncol 2011;29:4279-85.
9. Vogelstein B, Kinzler KW. Digital PCR. Proc Natl Acad Sci U S A 1999;96:9236-41. (Pubitemid 29374738)
10. Pohl G, Shih I. Principle and applications of digital PCR. Expert Rev Mol Diagn 2004;4:41-7. (Pubitemid 38089641)
11. Weaver S, Dube S, Mir A, Qin J, Sun G, Ramakrishnan R, et al. Taking qPCR to a higher level: Analysis of CNV reveals the power of high throughput qPCR to enhance quantitative resolution. Methods 2010;50:271-6.
12. White RA, Blainey PC, Fan HC, Quake SR. Digital PCR provides sensitive and absolute calibration for high throughput sequencing. BMC Genomics 2009;19:116.
13. Matsubara Y, Kerman K, Kobayashi M, Yamamura S, Morita Y, Tamiya E. Microchamber array based DNA quantification and specific sequence detection from a single copy via PCR in nanoliter volumes. Biosens Bioelectron 2005;20:1482-90. (Pubitemid 40051235)
14. Brenan CJ, Roberts D, Hurley J. Nanoliter high-throughput PCR for DNA and RNA profiling. Methods Mol Biol 2009;496:161-74.
15. Kiss MM, Ortoleva-Donnelly L, Beer NR, Warner J, Bailey CG, Colston BW, et al. High-throughput quantitative polymerase chain reaction in picoliter droplets. Anal Chem 2008;80:8975-81.
16. Markey AL, Mohr S, Day PJ. High-throughput droplet PCR. Methods 2010;50:277-81.
17. Hindson BJ, Ness KD, Masquelier DA, Belgrader P, Heredia NJ, Makarewicz AJ, et al. High-throughput droplet digital PCR system for absolute quantitation of DNA copy number. Anal Chem 2011;15:8604-10.
18. Heredia NJ, Belgrader P, Wang S, Koehler R, Regan J, Cosman AM, et al. Droplet Digital™ PCR quantitation of HER2 expression in FFPE breast tissue samples. Methods 2013;59:183-6.
19. Bartlett JM, Ibrahim M, Jasani B, Morgan JM, Ellis I, Kay E, et al. External quality assurance of HER2 FISH and ISH testing: three years of the UK National External Quality Assurance Scheme. Am J Clin Pathol 2009;131:106-11.