A new computer-controlled air-liquid interface cultivation system for the generation of differentiated cell cultures of the airway epithelium

Experimental and Toxicologic Pathology

Volumn 68, Issue 1, 2016, Pages 77-87

  Aufderheide, Michaela ^a   Förster, Christine ^b   Beschay, Morris ^c   Branscheid, Detlev ^c   Emura, Makito ^a  

^a Cultex Laboratories GmbH   (Germany)

^b NORDSTADT HOSPITAL   (Germany)

^c EVANGELISCHES KRANKENHAUS   (Germany)

Author keywords

Air liquid interface (ALI) cultures; Computer controlled long term cultivation system; CULTEX LTC C system; Differentiation; Normal human bronchial epithelial (NHBE) cells

Indexed keywords

AGED; ARTICLE; AUTOMATION; BIOSENSOR; BRONCHUS MUCOSA; BRONCHUS MUCUS; CELL CULTURE; CELL CULTURE EQUIPMENT; CELL DIFFERENTIATION ASSAY; CELL ISOLATION; CELL MEMBRANE; CELL STRUCTURE; CELLULAR SECRETION; COMPUTER SIMULATION; COMPUTER-CONTROLLED AIR-LIQUID INTERFACE CULTIVATION SYSTEM; CONTROLLED STUDY; EPITHELIUM CELL; HUMAN; HUMAN CELL; HUMAN TISSUE; IN VITRO STUDY; IN VIVO STUDY; MALE; MUCOSA CELL; POROSITY; PSEUDOSTRATIFIED ORGANIZATION; THREE DIMENSIONAL IMAGING; BRONCHUS; CELL CULTURE TECHNIQUE; COMPUTER; CYTOLOGY; DEVICES; IMMUNOHISTOCHEMISTRY; LABORATORY AUTOMATION; PROCEDURES; RESPIRATORY TRACT MUCOSA;

AGED; AUTOMATION, LABORATORY; BRONCHI; CELL CULTURE TECHNIQUES; COMPUTERS; HUMANS; IMMUNOHISTOCHEMISTRY; MALE; RESPIRATORY MUCOSA;

EID: 84951149774     PISSN: 09402993     EISSN: 16181433     Source Type: Journal    
DOI: 10.1016/j.etp.2015.10.001     Document Type: Article

References (20)

1. Abaci H.E., Devendra R., Smith Q., Gerecht S., Drazer G. Design and development of microbioreact ors for long-term cell culture in controlled oxygen microenvironments. Biomed. Microdevices 2012, 14:145-152.
2. Abbott R.D., Kaplan D.L. Strategies for improving the physiological relevance of human engineered tissues. Trends Biotechnol. 2015, 33:401-407.
3. Adler S., Basketter D., Creton S., Pelkonen O., van Benthem J., Zuang V., Andersen K.E., Angers-Loustau A., Aptula A., Bal-Price A., Benfenati E., Bernauer U., Bessems J., Bois F.Y., Boobis A., Brandon E., Bremer S., Broschard T., Casati S., Coecke S., Corvi R., Cronin M., Daston G., Dekant W., Felter S., Grignard E., Gundert-Remy U., Heinonen T., Kimber I., Kleinjans J., Komulainen H., Kreiling R., Kreysa J., Leite S.B., Loizou G., Maxwell G., Mazzatorta P., Munn S., Pfuhler S., Phrakonkham P., Piersma A., Poth A., Prieto P., Repetto G., Rogiers V., Schoeters G., Schwarz M., Serafimova R., Tähti H., Testai E., van Delft J., van Loveren H., Vinken M., Worth A., Zaldivar J.M. Alternative (non-animal) methods for cosmetics testing: current status and future prospects-2010. Arch. Toxicol. 2011, 85:367-485.
4. Anton F., Suck K., Diederichs S., Hitzmann B., van Griensven M., Scheüer T., Kasper C. Design and characterization of a rotating bioreactor for tissue engineering applications. Biotechnol. Prog. 2008, 24:140-147.
5. Collins F.S., Gray G.M., Bucher J.R. Transforming environmental health protection. Science 2008, 319:906-907.
6. Emura M., Aufderheide M., Mohr U. Target cell types with stem/progenitor function to isolate for in vitro reconstruction of human bronchiolar epithelia. Exp. Toxicol. Pathol. 2015, 67(2):81-88.
7. Ghaedi M., Mendez J.J., Bove P.F., Sivarapatna A., Raredon M.S., Niklason L.E. Alveolar epithelial differentiation of human induced pluripotent stem cells in a rotating bioreactor. Biomaterials 2014, 35:699-710.
8. Haldankar R., Li D., Saremi Z., Baikalov C., Deshpande R. Serum-free suspension large-scale transient transfection of CHO cells in WAVE bioreactors. Mol. Biotechnol. 2006, 34:191-200.
9. Hartung T., Sabbioni E. Alternative in vitro assays in nanomaterial toxicology. Wiley Interdiscip. Rev. Nanomed. Nanobiotechnol. 2011, 3:545-573.
10. Johansson H., Lindstedt M., Albrekt A., Borrebaeck C. A genomic biomarker signature can predict skin sensitizers using a cell-based in vitro alternative to animal tests. BMC Genomics 2011, 12:399.
11. Kasper C., Suck K., Anton F., Scheper T., Kall S., van Griensven M. A newly developed rotating bed bioreactor system for bone tissue engineering. Topics in Tissue Engineering 2007, vol. 3. Chapter 7.
12. Kempner M., Felder R. A review of cell culture automation. JALA 2002, 7:56-62.
13. Minuth W.W., Denk L., Glashauser A. A modular culture system for the generation of multiple specialized tissues. Biomaterials 2010, 31:2945-2954.
14. Olmer R., Lange A., Selzer S., Kasper C., Haverich A., Martin U., Zweigerdt R. Suspension culture of human pluripotent stem cells in controlled, stirred bioreactors. Tissue Eng. Part C Methods 2012, 18:772-784.
15. Sbrana T., Ahluwalia A. Engineering Quasi-Vivo in vitro organ models. Adv. Exp. Med. Biol. 2012, 745:138-153.
16. Serra M., Brito C., Sousa M.F., Jensen J., Tostões R., Clemente J., Strehl R., Hyllner J., Carrondo M.J., Alves P.M. Improving expansion of pluripotent human embryonic stem cells in perfused bioreactors through oxygen control. J. Biotechnol. 2010, 148:208-215.
17. Siti-ismail N., Samadikuchaksaraei A., Bishop A.E., Polak J.M., Mantalaris A. Development of a novel three-dimensional, automatable and integrated bioprocess for the differentiation of embryonic stem cells into pulmonary alveolar cells in a rotating vessel bioreactor system. Tissue Eng. Part C Methods 2012, 18:263-272.
18. Suck K., Roeker S., Diederichs S., Anton F., Sanz-Herrera J.A., Ochoa I., Doblare M., Scheper T., van Griensven M., Kasper C. A rotating bed system bioreactor enables cultivation of primary osteoblasts on well-characterized Sponceram regarding structural and flow properties. Biotechnol. Prog. 2010, 26:671-678.
19. Voisin C., Aerts C., Jakubczak E., Houdret J.L., Tonnel T.B. Effects of nitrogen dioxide on alveolar macrophages surviving in the gas phase: a new experimental model for the study of in vitro cytotoxicity of toxic gases. Bull. Eur. Physiopath. Respir. 1977, 13:137-144.
20. Wurm F.M. Production of recombinant protein therapeutics in cultivated mammalian cells. Nat. Biotechnol. 2004, 22:1393-1398.