Structure-function relationships in Gan42B, an intracellular GH42 β-galactosidase from Geobacillus stearothermophilus

Acta Crystallographica Section D: Biological Crystallography

Volumn 71, Issue , 2015, Pages 2433-2448

  Solomon, Hodaya V ^a   Tabachnikov, Orly ^b   Lansky, Shifra ^a   Salama, Rachel ^b   Feinberg, Hadar ^a   Shoham, Yuval ^b   Shoham, Gil ^a  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

^b TECHNION ISRAEL INSTITUTE OF TECHNOLOGY   (Israel)

Author keywords

Catalytic mutant; Galactan utilization; Galactosaccharides; Galactose; Geobacillus stearothermophilus; GH42; Glycoside hydrolase; Glycosynthase; Homotrimer; galactosidase

Indexed keywords

4 NITROPHENYLGALACTOSIDE; 4-NITROPHENYLGALACTOSIDE; BACTERIAL PROTEIN; BETA GALACTOSIDASE; GALACTOSE; OLIGOSACCHARIDE; PROTEIN BINDING; PROTEIN SUBUNIT; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME ACTIVE SITE; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; GEOBACILLUS STEAROTHERMOPHILUS; KINETICS; METABOLISM; MOLECULAR GENETICS; MUTATION; PROTEIN MULTIMERIZATION; PROTEIN SECONDARY STRUCTURE; PROTEIN SUBUNIT; SEQUENCE ALIGNMENT; STRUCTURAL HOMOLOGY; STRUCTURE ACTIVITY RELATION; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BETA-GALACTOSIDASE; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; GALACTOSE; GENE EXPRESSION; GEOBACILLUS STEAROTHERMOPHILUS; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; NITROPHENYLGALACTOSIDES; OLIGOSACCHARIDES; PROTEIN BINDING; PROTEIN MULTIMERIZATION; PROTEIN STRUCTURE, SECONDARY; PROTEIN SUBUNITS; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; STRUCTURAL HOMOLOGY, PROTEIN; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 84948798341     PISSN: 09074449     EISSN: 13990047     Source Type: Journal    
DOI: 10.1107/S1399004715018672     Document Type: Article

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