Biochemical characterization and identification of catalytic residues in α-glucuronidase from Bacillus stearothermophilus T-6

European Journal of Biochemistry

Volumn 268, Issue 10, 2001, Pages 3006-3016

  Zaide, Galia ^a   Shallom, Dalia ^a   Shulami, Smadar ^a   Zolotnitsky, Gennady ^a   Golan, Gali ^b   Baasov, Timor ^a   Shoham, Gil ^b   Shoham, Yuval ^a  

^a TECHNION ISRAEL INSTITUTE OF TECHNOLOGY   (Israel)

^b HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

D glucuronidase; Bacillus stearothermophilus; Family 67 glycosyl hydrolases; Inverting mechanism; Xylan

Indexed keywords

ALDOTETRAOURONIC ACID; ALPHA D GLUCURONIDASE; AMINO ACID; ASPARTIC ACID; CYSTEINE; ENZYME; GLUTAMIC ACID; METAL ION; MUTANT PROTEIN; PROTEIN SUBUNIT; UNCLASSIFIED DRUG; URONIC ACID;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DIFFERENTIAL SCANNING CALORIMETRY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME SPECIFICITY; GEL FILTRATION CHROMATOGRAPHY; GEOBACILLUS STEAROTHERMOPHILUS; HEAT TREATMENT; INCUBATION TIME; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DENATURATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS; STRAIN DIFFERENCE; TEMPERATURE DEPENDENCE; THERMOSTABILITY;

ALANINE; AMINO ACID SEQUENCE; ASPARTIC ACID; BACILLUS STEAROTHERMOPHILUS; BINDING SITES; CALORIMETRY, DIFFERENTIAL SCANNING; CATALYSIS; CATALYTIC DOMAIN; CHROMATOGRAPHY, GEL; CYSTEINE; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GLUTAMIC ACID; GLYCOSIDE HYDROLASES; HEAT; HYDROGEN-ION CONCENTRATION; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING; SEQUENCE ANALYSIS, PROTEIN; SEQUENCE HOMOLOGY, AMINO ACID; TEMPERATURE; TRIOSES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; GEOBACILLUS STEAROTHERMOPHILUS; POSIBACTERIA;

EID: 0034833426     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2001.02193.x     Document Type: Article

References (64)

1. Chemistry of hemicelluloses: Relationship between hemicllulose structure and enzymes required for hydrolysis
2. Biochemical aspects of the production of microbial hemicellulases
3. On the ester linkage between lignin and 4-O-methyl-D-glucurono-Dd-xylan in jute fiber (Corchorus capsularis)
4. Structure and function analysis of Pseudomonas plant cell wall hydrolases
5. Endo-beta-1,4-xylanase families: Differences in catalytic properties
6. Delignification of wood pulp by a thermostable xylanase from Bacillus stearothermophilus T-6
7. Hemicellulases in the bleaching of chemical pulps
8. Applications of hemicellulases in food, feed and pulp and paper industries
9. The acid hydrolysis of glycosides IX: Hydrolysis of two aldotriuronic acids derived from a (4-O-methyl glucurono)-xylan
10. The alpha-glucuronidase-encoding gene of Trichoderma reesei
11. Isolation and analysis of a gene encoding alpha-glucuronidase, an enzyme with a novel primary structure involved in the breakdown of xylan
12. aguA, the gene encoding an extracellular alpha-glucuronidase from Aspergillus tubingensis, is specifically induced on xylose and not on glucuronic acid
13. Gene cloning and characterization of alpha-glucuronidase of Bacillus stearothermo-philus no. 236
14. Updating the sequence-based classification of glycosyl hydrolases
15. Structural and sequence-based classification of glycoside hydrolases
16. Inverting character of alpha-glucuronidase A from Aspergillus tubingensis
17. Catalytic mechanisms of enzymic glycosyl transfer
18. The glucuronic acid utilization gene cluster from Bacillus stearothermophilus T-6
19. Crystallization and preliminary X-ray analysis of alpha-D-glucuronidase from Bacillus stearothermophilus T-6
20. Purification and characterization of a thermostable xylanase from Bacillus stearothermophilus T-6
21. A procedure for the isolation of deoxyribonucleic acid from micro-organisms
22. Genetic characterization
23. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs
24. Xylobiose and xylooligomers
25. A copper reagent for the determination of hexuronic acids and certain ketohexoses
26. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
27. Site-directed mutagenesis of virtually any plasmid by eliminating a unique site
28. The structure of signal peptides from bacterial lipoproteins
29. Relationship between opacity of transformed E. coli colonies and over-expression of the recombinant transcript
30. Crystallization and preliminary X-ray analysis of an intracellular xylanase from Bacillus stearothermophilus T-6
31. Cloning and DNA sequence of the gene coding for Bacillus stearothermophilus T-6 xylanase
32. Expression, purification, and characterization of the cellulose-binding domain of the scaffoldin subunit from the cellulosome of Clostridium thermocellum
33. alpha-D-glucuronidases from the xylanolytic thermophiles Clostridium stercorarium and Thermoanaerobacterium saccharolyticum
34. Purification and characterization of the α-glucuronidase from Thermoanaero-bacterium sp. strain JW/SL-YS485, an important enzyme for the utilization of substituted xylans
35. Purification and characterization of an alpha-D-glucuronidase from a thermophilic fungus, Thermoascus aurantiacus
36. α-Glucuronidases in the hydrolysis of wood xylans
37. Purification and characterization of intracellular α-glucuronidase from Aspergillus niger 5-16
38. An α-glucuronidase from Trichoderma reesei RUT C-30
39. Disulphide bonds between cysteine residues
40. Purification and characterization of α-glucuronidase from snail acetone powder
41. D-Xylan-degrading enzyme system from the fungus Phanerochaete chrysosporium: Isolation and partial characterisation of an alpha-(4-O-methyl)-D-glucuronidase
42. Microbial degradation of hemicellulosic materials
43. An alpha-glucuronidase of Schizophyllum commune acting on polymeric xylan
44. Substrate specificity of alpha-glucuronidase isolated from snail acetone powder
45. Isothermal titration calorimetry and differential scanning calorimetry as complementary tools to investigate the energetics of biomolecular recognition
46. Interaction of polysaccharides with the N-terminal cellulose-binding domain of Cellulomonas fimi CenC. 1. Binding specificity and calorimetric analysis
47. Isothermal titration calorimetric studies on the binding of deoxytrimannoside derivatives with artocarpin: Implications for a deep-seated combining site in lectins
48. Site-directed mutation of the putative catalytic residues of endoglucanase CenA from Cellulomonas fimi
49. Mutagenesis of glycosidases
50. Glycosyl transferase mechanisms
51. Catalytic mechanism of fungal glucoamylase as defined by mutagenesis of Asp176, Glu179 and Glu180 in the enzyme from Aspergillus awamori
52. Residues essential for catalytic activity of soybean beta-amylase
53. Refined structure for the complex of 1-deoxynojirimycin with glucoamylase from Aspergillus awamori var. X100-2.4-Å resolution
54. Crystal structures of soybean beta-amylase reacted with beta-maltose and maltal: Active site components and their apparent roles in catalysis
55. Site-directed mutagenesis of the catalytic base glutamic acid 400 in glucoamylase from Aspergillus niger and of tyrosine 48 and glutamine 401, both hydrogen-bonded to the gamma-carboxylate group of glutamic acid 400
56. Alpha-(4-O-methyl-D-glucuronidase activity produced by the rumen anaerobic fungus Piromonas communis: A study of selected properties
57. Mechanism of Agro-bacterium beta-glucosidase: Kinetic studies
58. A chromogenic substrate for a beta-xylosidase-coupled assay of alpha-glucuronidase