High-yield soluble expression, purification and characterization of human steroidogenic acute regulatory protein (StAR) fused to a cleavable Maltose-Binding Protein (MBP)

Protein Expression and Purification

Volumn 119, Issue , 2016, Pages 27-35

  Sluchanko, Nikolai N ^a   Tugaeva, Kristina V ^a,b   Faletrov, Yaroslav V ^c   Levitsky, Dmitrii I ^a,b  

^a BACH INSTITUTE OF BIOCHEMISTRY   (Russian Federation)

^b MOSCOW STATE UNIVERSITY   (Russian Federation)

^c BELARUSIAN STATE UNIVERSITY   (Belarus)

Author keywords

Cholesterol; Fluorescence; Maltose Binding Protein; Soluble expression; Steroid hormones; Steroidogenic acute regulatory protein

Indexed keywords

CHOLESTEROL; MALTOSE BINDING PROTEIN; PHOSPHOPROTEIN; PROTEIN BINDING; STEROIDOGENIC ACUTE REGULATORY PROTEIN;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; BIOSYNTHESIS; CHEMISTRY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; MOLECULAR CLONING; MOLECULAR GENETICS; SOLUBILITY;

AMINO ACID SEQUENCE; CHOLESTEROL; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; MALTOSE-BINDING PROTEINS; MOLECULAR SEQUENCE DATA; PHOSPHOPROTEINS; PROTEIN BINDING; SOLUBILITY;

EID: 84947982787     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2015.11.002     Document Type: Article

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