Characterization of monoclonal antibodies by a fast and easy liquid chromatography-mass spectrometry time-of-flight analysis on culture supernatant

Analytical Biochemistry

Volumn 491, Issue , 2015, Pages 52-54

  Henninot, Antoine ^a   Terrier, Aurelie ^b   Charton, Julie ^a   Urbain, Rémi ^b   Fontayne, Alexandre ^b   Deprez, Benoit ^a   Beghyn, Terence ^a  

^a INSTITUT PASTEUR DE LILLE   (France)

^b LFB Laboratoire Français du Fractionnement et des Biotechnologies   (France)

Author keywords

Fucosylation; Glycoform; Mass spectrometry; Monoclonal antibody

Indexed keywords

CAPILLARY ELECTROPHORESIS; MASS SPECTROMETRY; MONOCLONAL ANTIBODIES;

CULTURE SUPERNATANT; FUCOSYLATION; GLYCOFORMS; GOLD STANDARDS; HIGH PERFORMANCE CAPILLARY ELECTROPHORESIS; LASER INDUCED FLUORESCENCE DETECTION; LIQUID CHROMATOGRAPHY - MASS SPECTROMETRIES; TIME OF FLIGHT ANALYSIS;

LIQUID CHROMATOGRAPHY;

DIMETHYL SULFOXIDE; ENDOGLYCOSIDASE; GLYCAN; GLYCOFORM; GLYCOSIDASE; IMMUNOGLOBULIN G; ISOPROTEIN; MONOCLONAL ANTIBODY; PROTEINASE; REAGENT; UNCLASSIFIED DRUG;

ARTICLE; CAPILLARY ELECTROPHORESIS; CELL CULTURE; CONTROLLED STUDY; FLUORESCENCE; FUCOSYLATION; LASER; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR WEIGHT; PRIORITY JOURNAL; PROTEIN ANALYSIS; STRUCTURE ANALYSIS; SUPERNATANT; TIME OF FLIGHT MASS SPECTROMETRY; COMPARATIVE STUDY; GLYCOSYLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SPECTROFLUOROMETRY;

ANTIBODIES, MONOCLONAL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ELECTROPHORESIS, CAPILLARY; GLYCOSYLATION; PROTEIN ISOFORMS; SPECTROMETRY, FLUORESCENCE; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 84944050131     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2015.08.006     Document Type: Article

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