Trichosanthin suppresses the proliferation of glioma cells by inhibiting LGR5 expression and the Wnt/β-catenin signaling pathway

Oncology Reports

Volumn 34, Issue 6, 2015, Pages 2845-2852

  Miao, Junjie ^a   Jiang, Yilin ^a   Wang, Dongliang ^a   Zhou, Jingru ^a   Fan, Cungang ^a   Jiao, Feng ^a   Liu, Bo ^a   Zhang, Jun ^a   Wang, Yangshuo ^a   Zhang, Qingjun ^a  

^a PEKING UNIVERSITY PEOPLE S HOSPITAL   (China)

Author keywords

Apoptosis; Glioma; LGR5; Trichosanthin; Wnt catenin signaling

Indexed keywords

BETA CATENIN; CYCLIN D1; G PROTEIN COUPLED RECEPTOR; GLYCOGEN SYNTHASE KINASE 3BETA; LEUCINE RICH REPEAT CONTAINING G PROTEIN COUPLED RECEPTOR 5; MYC PROTEIN; TRICHOSANTHIN; UNCLASSIFIED DRUG; WNT PROTEIN; HERBACEOUS AGENT; LGR5 PROTEIN, HUMAN;

ANTINEOPLASTIC ACTIVITY; ANTIPROLIFERATIVE ACTIVITY; APOPTOSIS; ARTICLE; CANCER INHIBITION; CELL VIABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; DOSE TIME EFFECT RELATION; DOWN REGULATION; ENZYME PHOSPHORYLATION; GLIOMA CELL; HUMAN; HUMAN CELL; IN VITRO STUDY; INTRACELLULAR SIGNALING; MIGRATION INHIBITION; PLANT ROOT; PRIORITY JOURNAL; PROTEIN EXPRESSION; STAINING; TRICHOSANTHES; TRICHOSANTHES KIRILOWII; TUNEL ASSAY; WESTERN BLOTTING; WNT SIGNALING PATHWAY; BIOSYNTHESIS; CELL PROLIFERATION; CELL SURVIVAL; CHEMISTRY; DRUG EFFECTS; GENE EXPRESSION REGULATION; GENETICS; GLIOMA; PATHOLOGY; TUMOR CELL LINE;

APOPTOSIS; CELL LINE, TUMOR; CELL PROLIFERATION; CELL SURVIVAL; DRUGS, CHINESE HERBAL; GENE EXPRESSION REGULATION, NEOPLASTIC; GLIOMA; HUMANS; RECEPTORS, G-PROTEIN-COUPLED; TRICHOSANTHES; TRICHOSANTHIN; WNT SIGNALING PATHWAY;

EID: 84943742621     PISSN: 1021335X     EISSN: 17912431     Source Type: Journal    
DOI: 10.3892/or.2015.4290     Document Type: Article

References (38)

1. Jansen M, Yip S and Louis DN: Molecular pathology in adult gliomas: Diagnostic, prognostic, and predictive markers. Lancet Neurol 9: 717-726, 2010.
2. Wen PY and Kesari S: Malignant gliomas in adults. NEngl J Med 359: 492-507, 2008.
3. Fortin Ensign SP, Mathews IT, Symons MH, Berens ME and Tran NL: Implications of Rho GTPase signaling in glioma cell invasion and tumor progression. Front Oncol 3: 241, 2013.
4. Maraganore JM, Joseph M and Bailey MC: Purification and characterization of trichosanthin. Homology to the ricin A chain and implications as to mechanism of abortifacient activity. J Biol Chem 262: 11628-11633, 1987.
5. Zhang XJ and Wang JH: Homology of trichosanthin and ricin A chain. Nature 321: 477-478, 1986.
6. Sha O, Niu J, Ng TB, Cho EY, Fu X and Jiang W: Antitumor action of trichosanthin, a type 1 ribosome-inactivating protein, employed in traditional Chinese medicine: A mini review. Cancer Chemother Pharmacol 71: 1387-1393, 2013.
7. Zhang CY, Gong YX, Ma H, An CC and Chen DY: Trichosanthin induced calcium-dependent generation of reactive oxygen species in human choriocarcinoma cells. Analyst 125: 1539-1542, 2000.
8. Zhang C, Gong Y, Ma H, An C, Chen D and Chen ZL: Reactive oxygen species involved in trichosanthin-induced apoptosis of human choriocarcinoma cells. Biochem J 355: 653-661, 2001.
9. Wang P, Chen LL, Yan H and Li JC: Trichosanthin suppresses HeLa cell proliferation through inhibition of the PKC/MAPK signaling pathway. Cell Biol Toxicol 25: 479-488, 2009.
10. Wang P, Xu S, Zhao K, Xiao B and Guo J: Increase in cytosolic calcium maintains plasma membrane integrity through the formation of microtubule ring structure in apoptotic cervical cancer cells induced by trichosanthin. Cell Biol Int 33: 1149-1154, 2009.
11. Cui L, Song J, Wu L, Huang L, Wang Y, Huang Y, Yu H, Huang Y, You CC and Ye J: Smac is another pathway in the anti-tumour activity of trichosanthin and reverses trichosanthin resistance in CaSki cervical cancer cells. Biomed Pharmacother 69: 119-124, 2015.
12. Dou CM and Li JC: Effect of extracts of trichosanthes root tubers on HepA-H cells and HeLa cells. World J Gastroenterol 10: 2091-2094, 2004.
13. Fang EF, Zhang CZ, Zhang L, Wong JH, Chan YS, Pan WL, Dan XL, Yin CM, Cho CH and Ng TB: Trichosanthin inhibits breast cancer cell proliferation in both cell lines and nude mice by promotion of apoptosis. PLoS One 7: e41592, 2012.
14. Zheng YT, Zhang WF, Ben KL and Wang JH: In vitro immunotoxicity and cytotoxicity of trichosanthin against human normal immunocytes and leukemia-lymphoma cells. Immunopharmacol Immunotoxicol 17: 69-79, 1995.
15. Li J, Xia X, Ke Y, Nie H, Smith MA and Zhu X: Trichosanthin induced apoptosis in HL-60 cells via mitochondrial and endoplasmic reticulum stress signaling pathways. Biochim Biophys Acta 1770: 1169-1180, 2007.
16. Li J, Xia X, Nie H, Smith MA and Zhu X: PKC inhibition is involved in trichosanthin-induced apoptosis in human chronic myeloid leukemia cell line K562. Biochim Biophys Acta 1770: 63-70, 2007.
17. Byers VS, Levin AS, Malvino A, Waites L, Robins RA and Baldwin RW: A phase II study of effect of addition of trichosanthin to zidovudine in patients with HIV disease and failing antiretroviral agents. AIDS Res Hum Retroviruses 10: 413-420, 1994.
18. McGrath MS, Hwang KM, Caldwell SE, Gaston I, Luk KC, Wu P, Ng VL, Crowe S, Daniels J and Marsh J: GLQ223: An inhibitor of human immunodeficiency virus replication in acutely and chronically infected cells of lymphocyte and mononuclear phagocyte lineage. Proc Natl Acad Sci USA 86: 2844-2848, 1989.
19. Yang N, Li Z, Jiao Z, Gu P, Zhou Y, Lu L and Chou KY: A Trichosanthin-derived peptide suppresses type 1 immune responses by TLR2-dependent activation of CD8(+)CD28(-) Tregs. Clin Immunol 153: 277-287, 2014.
20. Tang NL, Chan WL, Ke YO, Mak MK, Lai FM and Tam SC: Acute renal failure and proximal tubule lesions after trichosanthin injection in rats. Exp Mol Pathol 64: 78-89, 1997.
21. Li F, Mei Y, Wang Y, Chen C, Tu J, Xiao B and Xu L: Trichosanthin inhibits antigen-specific T cell expansion through nitric oxide-mediated apoptosis pathway. Cell Immunol 234: 23-30, 2005.
22. Ng TB, Liu WK, Tsao SW and Yeung HW: Effect of trichosanthin and momorcharins on isolated rat hepatocytes. J Ethnopharmacol 3: 81-87, 1994.
23. Garcia PA, Bredesen DE, Vinters HV, Graefin von Einsiedel R, Williams RL, Kahn JO, Byers VS, Levin AS, Waites LA and Messing RO: Neurological reactions in HIV-infected patients treated with trichosanthin. Neuropathol Appl Neurobiol 19: 402-405, 1993.
24. Ng TB, Kwong WH and Yeung HW: Intravenous injections of the ribosome inactivating protein trichosanthin did not affect methionine enkephalin and β-endorphin levels in the mouse brain and pituitary. Biochem Mol Biol Int 39: 985-989, 1996.
25. Rot S, Taubert H, Bache M, Greither T, Würl P, Eckert AW, Schubert J, Vordermark D and Kappler M: A novel splice variant of the stem cell marker LGR5/GPR49 is correlated with the risk of tumor-related death in soft-tissue sarcoma patients. BMC Cancer 11: 429, 2011.
26. Stewart LA: Chemotherapy in adult high-grade glioma: A systematic review and meta-analysis of individual patient data from 12 randomised trials. Lancet 359: 1011-1018, 2002.
27. Bleehen NM and Stenning SP; The Medical Research Council Brain Tumour Working Party: A Medical Research Council trial of two radiotherapy doses in the treatment of grades 3 and 4 astrocytoma. Br J Cancer 64: 769-774, 1991.
28. Hsu SY, Liang SG and Hsueh AJ: Characterization of two LGR genes homologous to gonadotropin and thyrotropin receptors with extracellular leucine-rich repeats and a Gprotein-coupled, seven-transmembrane region. Mol Endocrinol 12: 1830-1845, 1998.
29. Hsu SY, Kudo M, Chen T, Nakabayashi K, Bhalla A, van der Spek PJ, van Duin M and Hsueh AJ: The three subfamilies of leucine-rich repeat-containing Gprotein-coupled receptors (LGR): Identification of LGR6 and LGR7 and the signaling mechanism for LGR7. Mol Endocrinol 14: 1257-1271, 2000.
30. Barker N and Clevers H: Leucine-rich repeat-containing G-protein-coupled receptors as markers of adult stem cells. Gastroenterology 138: 1681-1696, 2010.
31. McClanahan T, Koseoglu S, Smith K, Grein J, Gustafson E, Black S, Kirschmeier P and Samatar AA: Identification of overexpression of orphan Gprotein-coupled receptor GPR49 in human colon and ovarian primary tumors. Cancer Biol Ther 5: 419-426, 2006.
32. Scannell CA, Pedersen EA, Mosher JT, Krook MA, Nicholls LA, Wilky BA, Loeb DM and Lawlor ER: LGR5 is expressed by Ewing sarcoma and potentiates Wnt/beta-catenin signaling. Front Oncol 3: 81, 2013.
33. Nakata S, Campos B, Bageritz J, Bermejo JL, Becker N, Engel F, Acker T, Momma S, Herold-Mende C, Lichter P, et al: LGR5 is a marker of poor prognosis in glioblastoma and is required for survival of brain cancer stem-like cells. Brain Pathol 23: 60-72, 2013.
34. Wang D, Zhou J, Fan C, Jiao F, Liu B, Sun P, Miao J and Zhang Q: Knockdown of LGR5 suppresses the proliferation of glioma cells in vitro and in vivo. Oncol Rep 31: 41-49, 2014.
35. Wend P, Holland JD, Ziebold U and Birchmeier W: Wnt signaling in stem and cancer stem cells. Semin Cell Dev Biol 21: 855-863, 2010.
36. Glinka A, Dolde C, Kirsch N, Huang YL, Kazanskaya O, Ingelfinger D, Boutros M, Cruciat CM and Niehrs C: LGR4 and LGR5 are R-spondin receptors mediating Wnt/β-catenin and Wnt/PCP signalling. EMBO Rep 12: 1055-1061, 2011.
37. Anna CH, Iida M, Sills RC and Devereux TR: Expression of potential beta-catenin targets, cyclin D1, c-Jun, c-Myc, E-cadherin, and EGFR in chemically induced hepatocellular neoplasms from B6C3F1 mice. Toxicol Appl Pharmacol 190: 135-145, 2003.
38. Hsu HC, Liu YS, Tseng KC, Tan BC, Chen SJ and Chen HC: LGR5 regulates survival through mitochondria-mediated apoptosis and by targeting the Wnt/β-catenin signaling pathway in colorectal cancer cells. Cell Signal 26: 2333-2342, 2014.