Imidazole-free purification of His3-tagged recombinant proteins using ssDNA aptamer-based affinity chromatography

Journal of Chromatography A

Volumn 1418, Issue , 2015, Pages 130-139

  Bartnicki, Filip ^a   Kowalska, Ewa ^a   Pels, Katarzyna ^a   Strzalka, Wojciech ^a  

^a JAGIELLONIAN UNIVERSITY   (Poland)

Author keywords

DNA aptamers; Histidine tag; IMAC; Imidazole; SELEX

Indexed keywords

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; ESCHERICHIA COLI; ION CHROMATOGRAPHY; METAL IONS; METALS; PURIFICATION; RESINS;

DNA APTAMERS; HISTIDINE TAG; IMAC; IMIDAZOLE; SELEX;

RECOMBINANT PROTEINS;

APTAMER; BUFFER; IMIDAZOLE; POLYHISTIDINE TAG; RECOMBINANT PROTEIN; RESIN; SINGLE STRANDED DNA; SODIUM ION; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; HISTIDINE;

AFFINITY CHROMATOGRAPHY; ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DNA SEQUENCE; ESCHERICHIA COLI; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STABILITY; CHEMISTRY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PROCEDURES; PROTEIN SECONDARY STRUCTURE;

APTAMERS, NUCLEOTIDE; BASE SEQUENCE; CHROMATOGRAPHY, AFFINITY; DNA, SINGLE-STRANDED; ESCHERICHIA COLI; HISTIDINE; HUMANS; INDICATORS AND REAGENTS; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS;

EID: 84943396803     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2015.09.055     Document Type: Article

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