-
1
-
-
20144387833
-
Robotic cloning and protein production platform of the northeast structural genomics consortium
-
Acton T.B., Gunsalus K.C., Xiao R., Ma L.C., Aramini J., Baran M.C., Chiang Y.-W., Climent T., Cooper B., Denissova N.G., Douglas S.M., Everett J.K., et al. Robotic cloning and protein production platform of the northeast structural genomics consortium. Methods Enzymol. 394 (2005) 210-243
-
(2005)
Methods Enzymol.
, vol.394
, pp. 210-243
-
-
Acton, T.B.1
Gunsalus, K.C.2
Xiao, R.3
Ma, L.C.4
Aramini, J.5
Baran, M.C.6
Chiang, Y.-W.7
Climent, T.8
Cooper, B.9
Denissova, N.G.10
Douglas, S.M.11
Everett, J.K.12
-
3
-
-
33646857665
-
Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins
-
Arnau J., Lauritzen C., Petersen G.E., and Pedersen J. Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins. Protein Expr. Purif. 48 (2006) 1-13
-
(2006)
Protein Expr. Purif.
, vol.48
, pp. 1-13
-
-
Arnau, J.1
Lauritzen, C.2
Petersen, G.E.3
Pedersen, J.4
-
5
-
-
37349073090
-
Production and comprehensive quality control of recombinant human Interleukin-1β: A case study for a process development strategy
-
Block H., Kubicek J., Labahn J., Roth U., and Schäfer F. Production and comprehensive quality control of recombinant human Interleukin-1β: A case study for a process development strategy. Protein Expr. Purif. 27 (2008) 244-254
-
(2008)
Protein Expr. Purif.
, vol.27
, pp. 244-254
-
-
Block, H.1
Kubicek, J.2
Labahn, J.3
Roth, U.4
Schäfer, F.5
-
6
-
-
0028935311
-
Rapid one-step purification of goat immunoglobulins by immobilized metal ion affinity chromatography
-
Boden V., Winzerling J.J., Vijayalakshmi M., and Porath J. Rapid one-step purification of goat immunoglobulins by immobilized metal ion affinity chromatography. J. Immunol. Methods 181 (1995) 225-232
-
(1995)
J. Immunol. Methods
, vol.181
, pp. 225-232
-
-
Boden, V.1
Winzerling, J.J.2
Vijayalakshmi, M.3
Porath, J.4
-
7
-
-
33748578942
-
Structural analysis and classification of native proteins from E. coli commonly co-purified by immobilized metal affinity chromatography
-
Bolanos-Garcia V.M., and Davies O.R. Structural analysis and classification of native proteins from E. coli commonly co-purified by immobilized metal affinity chromatography. Biochim. Biophys. Acta 1760 (2006) 1304-1313
-
(2006)
Biochim. Biophys. Acta
, vol.1760
, pp. 1304-1313
-
-
Bolanos-Garcia, V.M.1
Davies, O.R.2
-
8
-
-
0033814819
-
Purification of protein using polyhistidine affinity tags
-
Bornhorst J.A., and Falke J.J. Purification of protein using polyhistidine affinity tags. Methods Enzymol. 326 (2000) 245-254
-
(2000)
Methods Enzymol.
, vol.326
, pp. 245-254
-
-
Bornhorst, J.A.1
Falke, J.J.2
-
9
-
-
0037022657
-
Proteome-scale purification of human proteins from bacteria
-
Braun P., Hu Y., Shen B., Halleck A., Koundinya M., Harlow E., and LaBaer J. Proteome-scale purification of human proteins from bacteria. Proc. Natl. Acad. Sci. USA 99 (2002) 2654-2659
-
(2002)
Proc. Natl. Acad. Sci. USA
, vol.99
, pp. 2654-2659
-
-
Braun, P.1
Hu, Y.2
Shen, B.3
Halleck, A.4
Koundinya, M.5
Harlow, E.6
LaBaer, J.7
-
11
-
-
0042954271
-
A human cDNA library for high-throughput protein expression screening
-
Büssow K., Nordhoff E., Lübbert C., Lehrach H., and Walter G. A human cDNA library for high-throughput protein expression screening. Genomics 65 (2000) 1-8
-
(2000)
Genomics
, vol.65
, pp. 1-8
-
-
Büssow, K.1
Nordhoff, E.2
Lübbert, C.3
Lehrach, H.4
Walter, G.5
-
12
-
-
33751293464
-
Solubilization and purification of membrane proteins
-
Lundstrom K. (Ed), CRC Press, Taylor and Francis, Boca Raton
-
Byrne B., and Jormakka M. Solubilization and purification of membrane proteins. In: Lundstrom K. (Ed). Structural Genomics on membrane proteins (2006), CRC Press, Taylor and Francis, Boca Raton 179-198
-
(2006)
Structural Genomics on membrane proteins
, pp. 179-198
-
-
Byrne, B.1
Jormakka, M.2
-
13
-
-
13844266546
-
Purification of recombinant proteins from mammalian cell culture using a generic double-affinity chromatography scheme
-
Cass B., Pham O.L., Kamen A., and Durocher Y. Purification of recombinant proteins from mammalian cell culture using a generic double-affinity chromatography scheme. Protein Expr. Purif. 40 (2005) 77-85
-
(2005)
Protein Expr. Purif.
, vol.40
, pp. 77-85
-
-
Cass, B.1
Pham, O.L.2
Kamen, A.3
Durocher, Y.4
-
14
-
-
31044446735
-
Rapid and easy thermodynamic optimization of the 5′-end of mRNA dramatically increases the level of wild type protein expression in Escherichia coli
-
Cèbe R., and Geiser M. Rapid and easy thermodynamic optimization of the 5′-end of mRNA dramatically increases the level of wild type protein expression in Escherichia coli. Protein Expr. Purif. 45 (2006) 374-380
-
(2006)
Protein Expr. Purif.
, vol.45
, pp. 374-380
-
-
Cèbe, R.1
Geiser, M.2
-
15
-
-
0033026779
-
2+-carboxymethylaspartate-agarose Superflow, as demonstrated by one-step purification of lactate dehydrogenase from chicken breast muscle
-
2+-carboxymethylaspartate-agarose Superflow, as demonstrated by one-step purification of lactate dehydrogenase from chicken breast muscle. Biotechnol. Appl. Biochem. 29 (1999) 19-24
-
(1999)
Biotechnol. Appl. Biochem.
, vol.29
, pp. 19-24
-
-
Chaga, G.1
Hopp, J.2
Nelson, P.3
-
16
-
-
0028248993
-
6xHis-Ni-NTA chromatography as a superior technique in recombinant protein expression/purification
-
Crowe J., Döbeli H., Gentz R., Hochuli E., Stüber D., and Henco K. 6xHis-Ni-NTA chromatography as a superior technique in recombinant protein expression/purification. Methods Mol. Biol. 31 (1994) 371-381
-
(1994)
Methods Mol. Biol.
, vol.31
, pp. 371-381
-
-
Crowe, J.1
Döbeli, H.2
Gentz, R.3
Hochuli, E.4
Stüber, D.5
Henco, K.6
-
17
-
-
0025341284
-
In vivo processing of N-terminal methionine in E. coli
-
Dalbøge H., Bayne S., and Pedersen J. In vivo processing of N-terminal methionine in E. coli. FEBS Lett 266 (1990) 1-3
-
(1990)
FEBS Lett
, vol.266
, pp. 1-3
-
-
Dalbøge, H.1
Bayne, S.2
Pedersen, J.3
-
18
-
-
0027482631
-
A single cDNA, hTFIIA/α, encodes both the p35 and p19 subunits of human TFIIA
-
DeJong J., and Roeder R.G. A single cDNA, hTFIIA/α, encodes both the p35 and p19 subunits of human TFIIA. Genes Dev. 7 (1993) 2220-2234
-
(1993)
Genes Dev.
, vol.7
, pp. 2220-2234
-
-
DeJong, J.1
Roeder, R.G.2
-
19
-
-
4344698577
-
The use of recombinant methods and molecular engineering in protein crystallization
-
Derewenda Z.S. The use of recombinant methods and molecular engineering in protein crystallization. Methods 34 (2004) 354-363
-
(2004)
Methods
, vol.34
, pp. 354-363
-
-
Derewenda, Z.S.1
-
20
-
-
0034677966
-
Drug discovery: A historical perspective
-
Drews J. Drug discovery: A historical perspective. Science 287 (2000) 1960-1964
-
(2000)
Science
, vol.287
, pp. 1960-1964
-
-
Drews, J.1
-
21
-
-
0023695530
-
Crystallization of trimeric recombinant human tumor necrosis factor (catechin)
-
Eck M.J., Beutler B., Kuo G., Merryweather J.P., and Sprang S.R. Crystallization of trimeric recombinant human tumor necrosis factor (catechin). J. Biol. Chem. 263 (1988) 12816-12819
-
(1988)
J. Biol. Chem.
, vol.263
, pp. 12816-12819
-
-
Eck, M.J.1
Beutler, B.2
Kuo, G.3
Merryweather, J.P.4
Sprang, S.R.5
-
22
-
-
14144256100
-
An efficient strategy for high-throughput expression of recombinant integral membrane proteins
-
Eshaghi S., Hedrén M., Ignatushchenko Abdel Nasser M., Hammarberg T., Thomell A., and Nordlund P. An efficient strategy for high-throughput expression of recombinant integral membrane proteins. Protein Sci. 14 (2005) 676-683
-
(2005)
Protein Sci.
, vol.14
, pp. 676-683
-
-
Eshaghi, S.1
Hedrén, M.2
Ignatushchenko Abdel Nasser, M.3
Hammarberg, T.4
Thomell, A.5
Nordlund, P.6
-
23
-
-
0034141690
-
Purification of His-tagged proteins by immobilized chelate affinity chromatography: The benefits from the use of organic solvents
-
Franken K.L.M.C., Hiemstra H.S., van Meijgaarden K.E., Subronto Y., den Hartigh J., Ottenhoff T.H.M., and Drijfthout J.W. Purification of His-tagged proteins by immobilized chelate affinity chromatography: The benefits from the use of organic solvents. Protein Expr. Purif. 18 (2000) 95-99
-
(2000)
Protein Expr. Purif.
, vol.18
, pp. 95-99
-
-
Franken, K.L.M.C.1
Hiemstra, H.S.2
van Meijgaarden, K.E.3
Subronto, Y.4
den Hartigh, J.5
Ottenhoff, T.H.M.6
Drijfthout, J.W.7
-
24
-
-
16544380488
-
DNA extraction Using Chelex resin for the oncogenic amplification analysis in head and neck tumors
-
García González L.A., Rodrigo Tapia J.P., Sánchez Lazo P., Ramos S., and Suárez Nieto C. DNA extraction Using Chelex resin for the oncogenic amplification analysis in head and neck tumors. Acta Otorrinolaringol. Esp. 55 (2004) 139-144
-
(2004)
Acta Otorrinolaringol. Esp.
, vol.55
, pp. 139-144
-
-
García González, L.A.1
Rodrigo Tapia, J.P.2
Sánchez Lazo, P.3
Ramos, S.4
Suárez Nieto, C.5
-
25
-
-
0043237327
-
Method to express and purify nm23-H2 protein from baculovirus-infected cells
-
Garzia L., André A., Amoresano A., D'Angelo A., Martusciello R., Cirulli C., Tsurumi T., Marino G., and Zollo M. Method to express and purify nm23-H2 protein from baculovirus-infected cells. BioTechniques 35 (2003) 384-391
-
(2003)
BioTechniques
, vol.35
, pp. 384-391
-
-
Garzia, L.1
André, A.2
Amoresano, A.3
D'Angelo, A.4
Martusciello, R.5
Cirulli, C.6
Tsurumi, T.7
Marino, G.8
Zollo, M.9
-
26
-
-
0026537853
-
A rapid polymerase chain reaction method for identifying fixed specimens
-
Gill P., Kimpton C.P., and Sullivan K. A rapid polymerase chain reaction method for identifying fixed specimens. Electrophoresis 13 (1992) 173-175
-
(1992)
Electrophoresis
, vol.13
, pp. 173-175
-
-
Gill, P.1
Kimpton, C.P.2
Sullivan, K.3
-
27
-
-
38749142906
-
Protein production and purification
-
Gräslund S., Nordlund P., Weigelt J., Bray J., Gileadi O., Knapp S., Oppermann U., Arrowsmith C., Hui R., Ming J., dhe-Paganon S., Park H.-W., et al. Protein production and purification. Nat. Methods 5 (2008) 135-146
-
(2008)
Nat. Methods
, vol.5
, pp. 135-146
-
-
Gräslund, S.1
Nordlund, P.2
Weigelt, J.3
Bray, J.4
Gileadi, O.5
Knapp, S.6
Oppermann, U.7
Arrowsmith, C.8
Hui, R.9
Ming, J.10
dhe-Paganon, S.11
Park, H.-W.12
-
28
-
-
0031260369
-
Quantitative evaluation of neurotensin receptor purification by immobilized metal affinity chromatography
-
Grisshammer R., and Tucker J. Quantitative evaluation of neurotensin receptor purification by immobilized metal affinity chromatography. Protein Expr. Purif. 11 (1997) 53-60
-
(1997)
Protein Expr. Purif.
, vol.11
, pp. 53-60
-
-
Grisshammer, R.1
Tucker, J.2
-
29
-
-
0027971005
-
Purification of humanized murine and murine monoclonal antibodies using immobilized metal-affinity chromatography
-
Hale J.E., and Beidler D.E. Purification of humanized murine and murine monoclonal antibodies using immobilized metal-affinity chromatography. Anal. Biochem. 222 (1994) 29-33
-
(1994)
Anal. Biochem.
, vol.222
, pp. 29-33
-
-
Hale, J.E.1
Beidler, D.E.2
-
30
-
-
0041048338
-
The configuration of cobaltihistidine and oxy-bis (cobalthistidine)
-
Hearon J. The configuration of cobaltihistidine and oxy-bis (cobalthistidine). J. Natl. Cancer Inst. 9 (1948) 1-11
-
(1948)
J. Natl. Cancer Inst.
, vol.9
, pp. 1-11
-
-
Hearon, J.1
-
31
-
-
0038739516
-
Protein purification by affinity precipitation
-
Hilbrig F., and Freitag R. Protein purification by affinity precipitation. J. Chromatogr. B 790 (2003) 79-90
-
(2003)
J. Chromatogr. B
, vol.790
, pp. 79-90
-
-
Hilbrig, F.1
Freitag, R.2
-
32
-
-
0344815737
-
Extent of N-terminal methionine excision from Escherichia coli proteins is governed by side-chain length of the penultimate amino acid
-
Hirel P.-H., Schmitter J.-M., Dessen P., Fayat G., and Blanquet S. Extent of N-terminal methionine excision from Escherichia coli proteins is governed by side-chain length of the penultimate amino acid. Proc. Natl. Acad. Sci. USA 86 (1989) 8247-8251
-
(1989)
Proc. Natl. Acad. Sci. USA
, vol.86
, pp. 8247-8251
-
-
Hirel, P.-H.1
Schmitter, J.-M.2
Dessen, P.3
Fayat, G.4
Blanquet, S.5
-
33
-
-
39149123785
-
Human protein atlas and the use of microarray technologies
-
Hober S., and Uhlén M. Human protein atlas and the use of microarray technologies. Curr. Opin. Biotechnol. 19 (2008) 30-35
-
(2008)
Curr. Opin. Biotechnol.
, vol.19
, pp. 30-35
-
-
Hober, S.1
Uhlén, M.2
-
34
-
-
0342348078
-
Genetically designed affinity chromatography using a novel metal chelate adsorbent
-
Hochuli E. Genetically designed affinity chromatography using a novel metal chelate adsorbent. Biologically Active Mol. 411 (1989) 217-239
-
(1989)
Biologically Active Mol.
, vol.411
, pp. 217-239
-
-
Hochuli, E.1
-
35
-
-
0023659137
-
New metal chelate adsorbent for proteins and peptides containing neighbouring histidine residues
-
Hochuli E., Döbeli H., and Schacher A. New metal chelate adsorbent for proteins and peptides containing neighbouring histidine residues. J. Chromatogr. 411 (1987) 177-184
-
(1987)
J. Chromatogr.
, vol.411
, pp. 177-184
-
-
Hochuli, E.1
Döbeli, H.2
Schacher, A.3
-
36
-
-
0023781763
-
Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsorbent
-
Hochuli E., Bannwarth W., Döbeli H., Gentz R., and Stüber D. Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsorbent. Biotechnology 6 (1988) 1321-1325
-
(1988)
Biotechnology
, vol.6
, pp. 1321-1325
-
-
Hochuli, E.1
Bannwarth, W.2
Döbeli, H.3
Gentz, R.4
Stüber, D.5
-
37
-
-
0019327676
-
Metal chelate affinity chromatography. I. Influence of various parameters on the retention of nucleotides and related compounds
-
Hubert P., and Porath J. Metal chelate affinity chromatography. I. Influence of various parameters on the retention of nucleotides and related compounds. J. Chromatogr. 198 (1980) 247-255
-
(1980)
J. Chromatogr.
, vol.198
, pp. 247-255
-
-
Hubert, P.1
Porath, J.2
-
38
-
-
0019444965
-
Metal chelate affinity chromatography. II. Group separation of mono- and dinucleotides
-
Hubert P., and Porath J. Metal chelate affinity chromatography. II. Group separation of mono- and dinucleotides. J. Chromatogr. 206 (1981) 164-168
-
(1981)
J. Chromatogr.
, vol.206
, pp. 164-168
-
-
Hubert, P.1
Porath, J.2
-
39
-
-
13844271129
-
From gene to protein: A review of new and enabling technologies for multi-parallel protein production
-
Hunt I. From gene to protein: A review of new and enabling technologies for multi-parallel protein production. Protein Expr. Purif. 40 (2005) 1-22
-
(2005)
Protein Expr. Purif.
, vol.40
, pp. 1-22
-
-
Hunt, I.1
-
40
-
-
0035016795
-
The neural RNA-binding protein Musashi1 translationally regulates mammalian numb gene expression by interacting with its mRNA
-
Imai T., Tokunaga A., Yoshida T., Hashimoto M., Mikoshiba K., Weinmaster G., Nakafuku M., and Okano H. The neural RNA-binding protein Musashi1 translationally regulates mammalian numb gene expression by interacting with its mRNA. Mol. Cell. Biol. 21 (2001) 3888-3900
-
(2001)
Mol. Cell. Biol.
, vol.21
, pp. 3888-3900
-
-
Imai, T.1
Tokunaga, A.2
Yoshida, T.3
Hashimoto, M.4
Mikoshiba, K.5
Weinmaster, G.6
Nakafuku, M.7
Okano, H.8
-
42
-
-
9144250356
-
Serological method using recombinant S2 protein to differentiate equine infectious anemia virus (EIAV)-infected and EIAV-vaccinated horses
-
Jin S., Issel C.J., and Montelaro R.C. Serological method using recombinant S2 protein to differentiate equine infectious anemia virus (EIAV)-infected and EIAV-vaccinated horses. Clin. Diagn. Lab. Immunol. 11 (2004) 1120-1129
-
(2004)
Clin. Diagn. Lab. Immunol.
, vol.11
, pp. 1120-1129
-
-
Jin, S.1
Issel, C.J.2
Montelaro, R.C.3
-
43
-
-
4644352412
-
Folding and refolding of proteins in chromatographic beds
-
Jungbauer A., Kaar W., and Schlegl R. Folding and refolding of proteins in chromatographic beds. Curr. Opin. Biotechnol. 15 (2004) 487-494
-
(2004)
Curr. Opin. Biotechnol.
, vol.15
, pp. 487-494
-
-
Jungbauer, A.1
Kaar, W.2
Schlegl, R.3
-
44
-
-
0028353230
-
Production, purification and immunogenicity of a Malaria transmission-blocking vaccine candidate: TBV25H expressed in yeast and purified using Ni-NTA Agarose
-
Kaslow D.C., and Shiloach J. Production, purification and immunogenicity of a Malaria transmission-blocking vaccine candidate: TBV25H expressed in yeast and purified using Ni-NTA Agarose. Biotechnology 12 (1994) 494-499
-
(1994)
Biotechnology
, vol.12
, pp. 494-499
-
-
Kaslow, D.C.1
Shiloach, J.2
-
45
-
-
31044454938
-
Cell-free synthesis and in situ isolation of recombinant proteins
-
Kim T.-W., Oh I.-S., Ahn J.-H., Choi C.-Y., and Kim D.-M. Cell-free synthesis and in situ isolation of recombinant proteins. Protein Expr. Purif. 45 (2006) 249-254
-
(2006)
Protein Expr. Purif.
, vol.45
, pp. 249-254
-
-
Kim, T.-W.1
Oh, I.-S.2
Ahn, J.-H.3
Choi, C.-Y.4
Kim, D.-M.5
-
46
-
-
46549089870
-
Western blot analysis using metal-nitrilotriacetate conjugated CdSe/ZnS quantum dots
-
Kim M.J., Park H.-Y., Kim J., Ryu J., Hong S., Han S.-J., and Song R. Western blot analysis using metal-nitrilotriacetate conjugated CdSe/ZnS quantum dots. Anal. Biochem. 379 (2008) 124-126
-
(2008)
Anal. Biochem.
, vol.379
, pp. 124-126
-
-
Kim, M.J.1
Park, H.-Y.2
Kim, J.3
Ryu, J.4
Hong, S.5
Han, S.-J.6
Song, R.7
-
47
-
-
28244458078
-
Evaluation of detergents for the soluble expression of α-helical and β-barrel-type integral membrane proteins by a preparative scale individual cell-free expression system
-
Klammt C., Schwarz D., Fendler K., Haase W., Dötsch V., and Bernhard F. Evaluation of detergents for the soluble expression of α-helical and β-barrel-type integral membrane proteins by a preparative scale individual cell-free expression system. FEBS J. 272 (2005) 6024-6038
-
(2005)
FEBS J.
, vol.272
, pp. 6024-6038
-
-
Klammt, C.1
Schwarz, D.2
Fendler, K.3
Haase, W.4
Dötsch, V.5
Bernhard, F.6
-
49
-
-
0035235252
-
Identification of conserved C2H2 zinc-finger gene families in the bilateria
-
Knight R.D., and Shimeld S.M. Identification of conserved C2H2 zinc-finger gene families in the bilateria. Genome Biol. 2 (2001) 1-8
-
(2001)
Genome Biol.
, vol.2
, pp. 1-8
-
-
Knight, R.D.1
Shimeld, S.M.2
-
50
-
-
84934436279
-
High-throughput protein production (HTPP): A review of enabling technologies to expedite protein production
-
In "High Throughput Protein Expression and Purification" (S. A. Doyle, ed.). Humana Press, Springer
-
Koehn J., and Hunt I. High-throughput protein production (HTPP): A review of enabling technologies to expedite protein production. Methods Mol. Biol. 498 (2009) 1-18 In "High Throughput Protein Expression and Purification" (S. A. Doyle, ed.). Humana Press, Springer
-
(2009)
Methods Mol. Biol.
, vol.498
, pp. 1-18
-
-
Koehn, J.1
Hunt, I.2
-
51
-
-
0033624216
-
6-tagged proteins allows exhaustive screening of libraries generated by random mutagenesis
-
6-tagged proteins allows exhaustive screening of libraries generated by random mutagenesis. BioTechniques 29 (2000) 338-342
-
(2000)
BioTechniques
, vol.29
, pp. 338-342
-
-
Lanio, T.1
Jeltsch, A.2
Pingoud, A.3
-
52
-
-
13844263242
-
Stable and functional immobilization of histidine-tagged proteins via multivalent chelator headgroups on a molecular poly(ethylene glycol) brush
-
Lata S., and Piehler J. Stable and functional immobilization of histidine-tagged proteins via multivalent chelator headgroups on a molecular poly(ethylene glycol) brush. Anal. Chem. 77 (2005) 1096-1105
-
(2005)
Anal. Chem.
, vol.77
, pp. 1096-1105
-
-
Lata, S.1
Piehler, J.2
-
53
-
-
33644516580
-
Specific and stable fluorescence labeling of histidine-tagged proteins for dissecting multi-protein complex formation
-
Lata S., Gavutis M., Tampé R., and Piehler J. Specific and stable fluorescence labeling of histidine-tagged proteins for dissecting multi-protein complex formation. J. Am. Chem. Soc. 128 (2006) 2365-2372
-
(2006)
J. Am. Chem. Soc.
, vol.128
, pp. 2365-2372
-
-
Lata, S.1
Gavutis, M.2
Tampé, R.3
Piehler, J.4
-
54
-
-
0034957669
-
High-throughput proteomics: Protein expression and purification in the postgenomic world
-
Lesley S.A. High-throughput proteomics: Protein expression and purification in the postgenomic world. Protein Expr. Purif. 22 (2001) 159-164
-
(2001)
Protein Expr. Purif.
, vol.22
, pp. 159-164
-
-
Lesley, S.A.1
-
55
-
-
0029072434
-
Validation studies in the regeneration of ion-exchange celluloses
-
Levison P.R., Badger S.E., Jones R.M.H., Toome D.W., Streater M., Pathirana N.D., and Wheeler S. Validation studies in the regeneration of ion-exchange celluloses. J. Chromatogr. A 702 (1995) 59-68
-
(1995)
J. Chromatogr. A
, vol.702
, pp. 59-68
-
-
Levison, P.R.1
Badger, S.E.2
Jones, R.M.H.3
Toome, D.W.4
Streater, M.5
Pathirana, N.D.6
Wheeler, S.7
-
56
-
-
39649087584
-
The funnel approach to the precrystallization production of membrane proteins
-
Lewinson O., Lee A.T., and Douglas C.R. The funnel approach to the precrystallization production of membrane proteins. J. Mol. Biol. 377 (2008) 62-73
-
(2008)
J. Mol. Biol.
, vol.377
, pp. 62-73
-
-
Lewinson, O.1
Lee, A.T.2
Douglas, C.R.3
-
57
-
-
0042421903
-
The tools of proteomics
-
Loo J.A. The tools of proteomics. Adv. Protein Chem. 65 (2003) 353-369
-
(2003)
Adv. Protein Chem.
, vol.65
, pp. 353-369
-
-
Loo, J.A.1
-
58
-
-
0141575314
-
Expression of milk-derived antihypertensive peptide in Escherichia coli
-
Lv G.S., Hua G.C., and Fu X.Y. Expression of milk-derived antihypertensive peptide in Escherichia coli. J. Dairy Sci. 86 (2003) 1927-1931
-
(2003)
J. Dairy Sci.
, vol.86
, pp. 1927-1931
-
-
Lv, G.S.1
Hua, G.C.2
Fu, X.Y.3
-
59
-
-
0027362247
-
Isolation of a cDNA encoding the largest subunit of TFIIA reveals functions important for activated transcription
-
Ma D., Watanabe H., Mermelstein F., Admon A., Oguri K., Sun X., Wada T., Imai T., Shiroya T., Reinberg D., and Handa H. Isolation of a cDNA encoding the largest subunit of TFIIA reveals functions important for activated transcription. Genes Dev. 7 (1993) 2246-2257
-
(1993)
Genes Dev.
, vol.7
, pp. 2246-2257
-
-
Ma, D.1
Watanabe, H.2
Mermelstein, F.3
Admon, A.4
Oguri, K.5
Sun, X.6
Wada, T.7
Imai, T.8
Shiroya, T.9
Reinberg, D.10
Handa, H.11
-
60
-
-
0035957867
-
Affinity chromatography of polyhistidine tagged enzymes: New dextran-coated immobilized metal ion affinity chromatography matrices for prevention of undesired multipoint adsorptions
-
Mateo C., Fernandez-Lorente G., Pessela B.C.C., Vian A., Carrascosa A.V., Garcia J.L., Fernandez-Lafuente R., and Guisan J.M. Affinity chromatography of polyhistidine tagged enzymes: New dextran-coated immobilized metal ion affinity chromatography matrices for prevention of undesired multipoint adsorptions. J. Chromatogr. A 915 (2001) 97-106
-
(2001)
J. Chromatogr. A
, vol.915
, pp. 97-106
-
-
Mateo, C.1
Fernandez-Lorente, G.2
Pessela, B.C.C.3
Vian, A.4
Carrascosa, A.V.5
Garcia, J.L.6
Fernandez-Lafuente, R.7
Guisan, J.M.8
-
61
-
-
34347255758
-
Metal-chelate affinity precipitation of proteins using responsive polymers
-
Matiasson B., Kumar A., Ivanov A.E., and Galaev I.Y. Metal-chelate affinity precipitation of proteins using responsive polymers. Nat. Protocols 2 (2007) 213-220
-
(2007)
Nat. Protocols
, vol.2
, pp. 213-220
-
-
Matiasson, B.1
Kumar, A.2
Ivanov, A.E.3
Galaev, I.Y.4
-
62
-
-
0041590995
-
Immobilized-metal affinity sorbents based on hydrophilic methacrylate polymers and their interaction with immunoglobulins
-
Mészárosová K., Tishchenko G., Bouchal K., and Bleha M. Immobilized-metal affinity sorbents based on hydrophilic methacrylate polymers and their interaction with immunoglobulins. React. Funct. Polym. 56 (2003) 27-35
-
(2003)
React. Funct. Polym.
, vol.56
, pp. 27-35
-
-
Mészárosová, K.1
Tishchenko, G.2
Bouchal, K.3
Bleha, M.4
-
63
-
-
0842288662
-
Membrane protein expression and production: Effects of polyhistidine tag length and position
-
Mohanty A.K., and Wiener M.C. Membrane protein expression and production: Effects of polyhistidine tag length and position. Protein Expr. Purif. 33 (2004) 311-325
-
(2004)
Protein Expr. Purif.
, vol.33
, pp. 311-325
-
-
Mohanty, A.K.1
Wiener, M.C.2
-
64
-
-
0023022190
-
Selective adsorption of phosphoproteins on gel-immobilized ferric chelate
-
Muszynska G., Andersson L., and Porath J. Selective adsorption of phosphoproteins on gel-immobilized ferric chelate. Biochemistry 25 (1986) 6850-6853
-
(1986)
Biochemistry
, vol.25
, pp. 6850-6853
-
-
Muszynska, G.1
Andersson, L.2
Porath, J.3
-
65
-
-
0031572843
-
Biacore analysis of histidine-tagged proteins using a chelating NTA sensor chip
-
Nieba L., Nieba-Axamann S.E., Persson A., Hämäläinen M., Edebratt F., Hansson A., Lidholm J., Magnusson K., Karlsson A.F., and Plückthun A. Biacore analysis of histidine-tagged proteins using a chelating NTA sensor chip. Anal. Biochem. 252 (1997) 217-228
-
(1997)
Anal. Biochem.
, vol.252
, pp. 217-228
-
-
Nieba, L.1
Nieba-Axamann, S.E.2
Persson, A.3
Hämäläinen, M.4
Edebratt, F.5
Hansson, A.6
Lidholm, J.7
Magnusson, K.8
Karlsson, A.F.9
Plückthun, A.10
-
66
-
-
0031259833
-
Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins
-
Nilsson J., Ståhl S., Lundeberg J., Uhlén M., and Nygren P.-A. Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins. Protein Expr. Purif. 11 (1997) 1-16
-
(1997)
Protein Expr. Purif.
, vol.11
, pp. 1-16
-
-
Nilsson, J.1
Ståhl, S.2
Lundeberg, J.3
Uhlén, M.4
Nygren, P.-A.5
-
68
-
-
0033117468
-
Removal of N-terminal polyhistidine tags from recombinant proteins using engineered aminopeptidases
-
Pedersen J., Lauritzen C., Madsen M.T., and Dahl S.W. Removal of N-terminal polyhistidine tags from recombinant proteins using engineered aminopeptidases. Protein Expr. Purif. 15 (1999) 389-400
-
(1999)
Protein Expr. Purif.
, vol.15
, pp. 389-400
-
-
Pedersen, J.1
Lauritzen, C.2
Madsen, M.T.3
Dahl, S.W.4
-
69
-
-
71549165870
-
-
Polayes, D.A., Parks, T.D., Johnston, S.A., and Dougherty, W.G. (2008). Application of TEV protease in protein production. Methods Mol. Med.13, 169-183. In Molecular Diagnosis of Infectious Diseases (U. Reischl. ed.). Humana Press, Springer
-
Polayes, D.A., Parks, T.D., Johnston, S.A., and Dougherty, W.G. (2008). Application of TEV protease in protein production. Methods Mol. Med.13, 169-183. In "Molecular Diagnosis of Infectious Diseases" (U. Reischl. ed.). Humana Press, Springer
-
-
-
-
70
-
-
0021114727
-
Immobilized metal ion affinity adsorption and immobilized metal ion affinity chromatography of biomaterials. Serum protein affinities for gel-immobilized iron and nickel ions
-
Porath J., and Olin B. Immobilized metal ion affinity adsorption and immobilized metal ion affinity chromatography of biomaterials. Serum protein affinities for gel-immobilized iron and nickel ions. Biochemistry 29 (1983) 1621-1630
-
(1983)
Biochemistry
, vol.29
, pp. 1621-1630
-
-
Porath, J.1
Olin, B.2
-
71
-
-
0016717761
-
Metal chelate affinity chromatography, a new approach to protein fractionation
-
Porath J., Carlsson J., Olsson I., and Belfrage G. Metal chelate affinity chromatography, a new approach to protein fractionation. Nature 258 (1975) 598-599
-
(1975)
Nature
, vol.258
, pp. 598-599
-
-
Porath, J.1
Carlsson, J.2
Olsson, I.3
Belfrage, G.4
-
72
-
-
0033565503
-
Immobilized gallium (III) affinity chromatography of phosphopeptides
-
Posewitz M.C., and Tempst P. Immobilized gallium (III) affinity chromatography of phosphopeptides. Anal. Chem. 71 (1999) 2883-2892
-
(1999)
Anal. Chem.
, vol.71
, pp. 2883-2892
-
-
Posewitz, M.C.1
Tempst, P.2
-
73
-
-
3543109683
-
Establishing a versatile fermentation procedure for human proteins expressed in the yeasts Saccharomyces cerevisiae and Pichia pastoris for structural genomics
-
Prinz B., Schultchen J., Rydzewski R., Holz C., Boettner M., Stahl U., and Lang C. Establishing a versatile fermentation procedure for human proteins expressed in the yeasts Saccharomyces cerevisiae and Pichia pastoris for structural genomics. J. Struct. Funct. Genomics 5 (2004) 29-44
-
(2004)
J. Struct. Funct. Genomics
, vol.5
, pp. 29-44
-
-
Prinz, B.1
Schultchen, J.2
Rydzewski, R.3
Holz, C.4
Boettner, M.5
Stahl, U.6
Lang, C.7
-
75
-
-
36448929836
-
Noncovalent, site-specific biotinylation of histidine-tagged proteins
-
Reichel A., Schaible D., Al Furoukh N., Cohen M., Schreiber G., and Piehler J. Noncovalent, site-specific biotinylation of histidine-tagged proteins. Anal. Chem. 79 (2007) 8590-8600
-
(2007)
Anal. Chem.
, vol.79
, pp. 8590-8600
-
-
Reichel, A.1
Schaible, D.2
Al Furoukh, N.3
Cohen, M.4
Schreiber, G.5
Piehler, J.6
-
76
-
-
0342378077
-
3 from Escherichia coli and demonstration that associated quinone is not required for the structural integrity of the oxidase
-
3 from Escherichia coli and demonstration that associated quinone is not required for the structural integrity of the oxidase. Biochim. Biophys. Acta 1340 (1997) 131-142
-
(1997)
Biochim. Biophys. Acta
, vol.1340
, pp. 131-142
-
-
Rumbley, J.N.1
Furlong Nickels, E.2
Gennis, R.B.3
-
77
-
-
0002572930
-
Ni-NTA for large-scale processes-systematic investigation of separation characteristics, storage and CIP conditions, and leaching. QIAGEN
-
Available from http:/{tab}{tab}/www1.qiagen.com/literature/qiagennews/0400/Ni-NTA%20for%20large-scale.pdf
-
Schäfer F., Blümer J., Römer U., and Steinert K. Ni-NTA for large-scale processes-systematic investigation of separation characteristics, storage and CIP conditions, and leaching. QIAGEN. QIAGEN News 4 (2000) 11-15 Available from http:/{tab}{tab}/www1.qiagen.com/literature/qiagennews/0400/Ni-NTA%20for%20large-scale.pdf
-
(2000)
QIAGEN News
, vol.4
, pp. 11-15
-
-
Schäfer, F.1
Blümer, J.2
Römer, U.3
Steinert, K.4
-
78
-
-
84867569623
-
A highly specific system for efficient enzymatic removal of tags from recombinant proteins
-
Schäfer F., Schäfer A., and Steinert K. A highly specific system for efficient enzymatic removal of tags from recombinant proteins. J. Biomol. Tech. 13 (2002) 158-171
-
(2002)
J. Biomol. Tech.
, vol.13
, pp. 158-171
-
-
Schäfer, F.1
Schäfer, A.2
Steinert, K.3
-
79
-
-
84867517334
-
Automated high-throughput purification of 6xHis-tagged proteins
-
Schäfer F., Römer U., Emmerlich M., Blümer J., Lubenow H., and Steinert K. Automated high-throughput purification of 6xHis-tagged proteins. J. Biomol. Tech. 13 (2002) 131-142
-
(2002)
J. Biomol. Tech.
, vol.13
, pp. 131-142
-
-
Schäfer, F.1
Römer, U.2
Emmerlich, M.3
Blümer, J.4
Lubenow, H.5
Steinert, K.6
-
80
-
-
3042566944
-
An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography
-
Available from www.biomedcentral.com/1472-6750/3/12
-
Scheich C., Sievert V., and Büssow K. An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography. BMC Biotechnol. 3 (2003) 12. http://www.biomedcentral.com/1472-6750/3/12 Available from www.biomedcentral.com/1472-6750/3/12
-
(2003)
BMC Biotechnol.
, vol.3
, pp. 12
-
-
Scheich, C.1
Sievert, V.2
Büssow, K.3
-
81
-
-
0027673618
-
Affinity purification of histidine-tagged proteins
-
Schmitt J., Hess H., and Stunnenberg H.G. Affinity purification of histidine-tagged proteins. Mol. Biol. Rep. 18 (1993) 223-230
-
(1993)
Mol. Biol. Rep.
, vol.18
, pp. 223-230
-
-
Schmitt, J.1
Hess, H.2
Stunnenberg, H.G.3
-
83
-
-
53049093022
-
Metallomics and metalloproteomics
-
Shi W., and Chance M.R. Metallomics and metalloproteomics. Cell. Mol. Life Sci. 65 (2008) 3040-3048
-
(2008)
Cell. Mol. Life Sci.
, vol.65
, pp. 3040-3048
-
-
Shi, W.1
Chance, M.R.2
-
84
-
-
0025943945
-
Transcriptional repression by YY1, a human GLI-Krüppel-related protein, and relief of repression by adenovirus E1A protein
-
Shi Y., Seto E., Chang L.-S., and Shenk T. Transcriptional repression by YY1, a human GLI-Krüppel-related protein, and relief of repression by adenovirus E1A protein. Cell 67 (1991) 377-388
-
(1991)
Cell
, vol.67
, pp. 377-388
-
-
Shi, Y.1
Seto, E.2
Chang, L.-S.3
Shenk, T.4
-
85
-
-
0037427798
-
Protein proteolysis and the multi-dimensional electrochromatographic separation of histidine-containing peptide fragments
-
Slentz B.E., Penner N.A., and Regnier F.E. Protein proteolysis and the multi-dimensional electrochromatographic separation of histidine-containing peptide fragments. J. Chromatogr. A 984 (2003) 97-103
-
(2003)
J. Chromatogr. A
, vol.984
, pp. 97-103
-
-
Slentz, B.E.1
Penner, N.A.2
Regnier, F.E.3
-
86
-
-
10644268451
-
Zooming in: Fractionation strategies in proteomics
-
Stasyk T., and Huber L.A. Zooming in: Fractionation strategies in proteomics. Proteomics 4 (2004) 3704-3716
-
(2004)
Proteomics
, vol.4
, pp. 3704-3716
-
-
Stasyk, T.1
Huber, L.A.2
-
87
-
-
33645417226
-
High-throughput protein purification using an automated set-up for high-yield affinity chromatography
-
Steen J., Uhlén M., Hober S., and Ottosson J. High-throughput protein purification using an automated set-up for high-yield affinity chromatography. Protein Expr. Purif. 46 (2006) 173-178
-
(2006)
Protein Expr. Purif.
, vol.46
, pp. 173-178
-
-
Steen, J.1
Uhlén, M.2
Hober, S.3
Ottosson, J.4
-
88
-
-
0035970019
-
Structural conformers produced during malaria vaccine production in yeast
-
Stowers A.W., Zhang Y., Shimp R.L., and Kaslow D.C. Structural conformers produced during malaria vaccine production in yeast. Yeast 18 (2001) 137-150
-
(2001)
Yeast
, vol.18
, pp. 137-150
-
-
Stowers, A.W.1
Zhang, Y.2
Shimp, R.L.3
Kaslow, D.C.4
-
90
-
-
0021967194
-
Purification of proteins by IMAC
-
Sulkowski E. Purification of proteins by IMAC. Trends Biotechnol. 3 (1985) 1-7
-
(1985)
Trends Biotechnol.
, vol.3
, pp. 1-7
-
-
Sulkowski, E.1
-
91
-
-
27144470222
-
Application of immobilized metal affinity chromatography in proteomics
-
Sun X., Chiu J.-F., and He Q.-Y. Application of immobilized metal affinity chromatography in proteomics. Expert Rev. Proteomics 2 (2005) 649-657
-
(2005)
Expert Rev. Proteomics
, vol.2
, pp. 649-657
-
-
Sun, X.1
Chiu, J.-F.2
He, Q.-Y.3
-
92
-
-
33646882130
-
Histidine tag fusions increases expression levels of active recombinant amelogenin in Escherichia coli
-
Svensson J., Andersson C., Reseland J.E., Lyngstadaas P., and Bülow L. Histidine tag fusions increases expression levels of active recombinant amelogenin in Escherichia coli. Protein Expr. Purif. 48 (2006) 134-141
-
(2006)
Protein Expr. Purif.
, vol.48
, pp. 134-141
-
-
Svensson, J.1
Andersson, C.2
Reseland, J.E.3
Lyngstadaas, P.4
Bülow, L.5
-
93
-
-
0037049890
-
IMAC of human IgG: Studies with IDA-immobilized copper, nickel, zinc, and cobalt ions and different buffer systems
-
Vancan S., Miranda E.A., and Bueno S.M.A. IMAC of human IgG: Studies with IDA-immobilized copper, nickel, zinc, and cobalt ions and different buffer systems. Process Biochem. 37 (2002) 573-579
-
(2002)
Process Biochem.
, vol.37
, pp. 573-579
-
-
Vancan, S.1
Miranda, E.A.2
Bueno, S.M.A.3
-
94
-
-
0025923821
-
Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material
-
Walsh P.S., Metzger D.A., and Higuchi R. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. BioTechniques 10 (1991) 506-513
-
(1991)
BioTechniques
, vol.10
, pp. 506-513
-
-
Walsh, P.S.1
Metzger, D.A.2
Higuchi, R.3
-
95
-
-
3543050105
-
His tag effect on solubility of human proteins produced in Escherichia coli: Comparison between four expression vectors
-
Woestenenk E.A., Hammarström M., van den Berg S., Härd T., and Berglund H. His tag effect on solubility of human proteins produced in Escherichia coli: Comparison between four expression vectors. J. Struct. Funct. Genomics 5 (2004) 217-229
-
(2004)
J. Struct. Funct. Genomics
, vol.5
, pp. 217-229
-
-
Woestenenk, E.A.1
Hammarström, M.2
van den Berg, S.3
Härd, T.4
Berglund, H.5
-
96
-
-
40849085149
-
Effect of the His-tag on the production of soluble and functional Buthus martensii Karsch insect toxin
-
Xu C.-G., Fan X.-J., Fu Y.-J., and Liang A.-H. Effect of the His-tag on the production of soluble and functional Buthus martensii Karsch insect toxin. Protein Expr. Purif. 59 (2008) 103-109
-
(2008)
Protein Expr. Purif.
, vol.59
, pp. 103-109
-
-
Xu, C.-G.1
Fan, X.-J.2
Fu, Y.-J.3
Liang, A.-H.4
-
97
-
-
33845222070
-
Facile synthesis of multivalent nitrilotriacetic acid (NTA) and NTA conjugates for analytical and drug delivery applications
-
Zhaohua H., Park J.I., Watson D.S., Hwang P., and Szoka F.C. Facile synthesis of multivalent nitrilotriacetic acid (NTA) and NTA conjugates for analytical and drug delivery applications. Bioconjug. Chem. 17 (2006) 1592-1600
-
(2006)
Bioconjug. Chem.
, vol.17
, pp. 1592-1600
-
-
Zhaohua, H.1
Park, J.I.2
Watson, D.S.3
Hwang, P.4
Szoka, F.C.5
-
98
-
-
33748328051
-
Zirconium phosphonate-modified porous silicon for highly specific capture of phosphopeptide and MALDI-TOF MS analysis
-
Zhou H., Xu S., Ye M., Feng S., Pan C., Jiang X., Li X., Han G., Fu Y., and Zou H. Zirconium phosphonate-modified porous silicon for highly specific capture of phosphopeptide and MALDI-TOF MS analysis. J. Proteome Res. 5 (2006) 2431-2437
-
(2006)
J. Proteome Res.
, vol.5
, pp. 2431-2437
-
-
Zhou, H.1
Xu, S.2
Ye, M.3
Feng, S.4
Pan, C.5
Jiang, X.6
Li, X.7
Han, G.8
Fu, Y.9
Zou, H.10
|