Gene stacking in plant cell using recombinases for gene integration and nucleases for marker gene deletion

BMC Biotechnology

Volumn 15, Issue 1, 2015, Pages

  Nandy, Soumen ^a   Zhao, Shan ^a   Pathak, Bhuvan P ^a   Manoharan, Muthusamy ^b   Srivastava, Vibha ^a  

^a UNIVERSITY OF ARKANSAS   (United States)

^b UNIVERSITY OF ARKANSAS AT PINE BLUFF   (United States)

Author keywords

Cre lox; FLP FRT; Gene stacking; I SceI; Multigene transformation; Site specific recombination; Targeted gene integration; ZFN

Indexed keywords

CHROMOSOMES; DNA SEQUENCES; EFFICIENCY; GENE ENCODING; INTEGRATION; PLANT CELL CULTURE; PLANTS (BOTANY); POLYMERASE CHAIN REACTION;

CRE LOXES; FLP-FRT; MULTIGENE TRANSFORMATION; SITE-SPECIFIC RECOMBINATION; ZFN;

GENES;

NUCLEASE; RECOMBINASE; CRE RECOMBINASE; DEOXYRIBONUCLEASE; GENETIC MARKER; INTEGRASE;

ARTICLE; CALLUS (PLANT); CHROMOSOME; CONTROLLED STUDY; CULTIVAR; DNA INTEGRATION; DNA SEQUENCE; ENZYME ACTIVITY; GENE CONSTRUCT; GENE DELETION; GENE INSERTION; GENE LOCUS; GENE STACKING; GENETIC ENGINEERING; GENETIC RECOMBINATION; INTROGRESSION; MARKER GENE; NONHUMAN; PLANT BREEDING; PLANT CELL; PLANT GENE; PLASMID; POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING; TRANSGENE; TRANSGENIC CROP; BIOTECHNOLOGY; GENE VECTOR; GENETIC MARKER; GENETICS; METABOLISM; ORYZA; PROCEDURES; TRANSGENIC PLANT;

BIOTECHNOLOGY; DEOXYRIBONUCLEASES; GENE DELETION; GENETIC ENGINEERING; GENETIC MARKERS; GENETIC VECTORS; INTEGRASES; ORYZA; PLANTS, GENETICALLY MODIFIED;

EID: 84943328246     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/s12896-015-0212-2     Document Type: Article

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