Exploring continuous and integrated strategies for the up- and downstream processing of human mesenchymal stem cells

Journal of Biotechnology

Volumn 213, Issue , 2015, Pages 97-108

  Cunha, Bárbara ^a,b   Aguiar, Tiago ^a,b   Silva, Marta M ^a,b   Silva, Ricardo J S ^a,b   Sousa, Marcos F Q ^a,b   Pineda, Earl ^c   Peixoto, Cristina ^a,b   Carrondo, Manuel J T ^b,d   Serra, Margarida ^a,b   Alves, Paula M ^a,b  

^a INSTITUTO DE TECNOLOGIA QUÍMICA E BIOLÓGICA   (Portugal)

^b INSTITUTO DE BIOLOGIA EXPERIMENTAL E TECNOLÓGICA   (Portugal)

^c REPLIGEN CORPORATION   (United States)

^d UNIVERSIDADE NOVA DE LISBOA   (Portugal)

Author keywords

Alternating tangential flow; Cell therapy; Mesenchymal stem cells; Perfusion; Process integration; Tangential flow filtration

Indexed keywords

CELL CULTURE; CELL DEATH; CELLS; CYTOLOGY; STEM CELLS;

CELL THERAPY; MESENCHYMAL STEM CELL; PERFUSION; PROCESS INTEGRATION; TANGENTIAL FLOW; TANGENTIAL FLOW FILTRATIONS;

MOLECULAR BIOLOGY;

LACTATE DEHYDROGENASE; TELOMERASE; AMMONIA; GLUCOSE; GLUTAMINE; LACTIC ACID;

APOPTOSIS; ARTICLE; CELL ADHESION; CELL DEATH; CELL DIFFERENTIATION; CELL GROWTH; CELL METABOLISM; CELL STRUCTURE; CELL VIABILITY; CONCENTRATION (PARAMETERS); CONTINUOUS CULTURE; CONTINUOUS PERFUSION CULTURE; CONTINUOUS STIRRED TANK REACTOR; CONTROLLED STUDY; DIAFILTRATION; DOWNSTREAM PROCESSING; FILTRATION; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; IMMUNOPHENOTYPING; MESENCHYMAL STEM CELL; MULTIPOTENT STEM CELL; PRIORITY JOURNAL; PROCESSING; REACTOR OPERATION; REAL TIME POLYMERASE CHAIN REACTION; STEM CELL EXPANSION; TANGENTIAL FLOW FILTRATION; TIME; UPSTREAM PROCESSING; BIOREACTOR; CELL COUNT; CELL CULTURE TECHNIQUE; CELL PROLIFERATION; CELL SURVIVAL; CYTOLOGY; FIBROBLAST; MALE; MESENCHYMAL STROMA CELL; METABOLISM; PERFUSION; PHYSIOLOGY; PREPUCE;

AMMONIA; BIOREACTORS; CELL ADHESION; CELL COUNT; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; CELL SURVIVAL; FIBROBLASTS; FORESKIN; GLUCOSE; GLUTAMINE; HUMANS; LACTIC ACID; MALE; MESENCHYMAL STROMAL CELLS; PERFUSION; TELOMERASE;

EID: 84942829160     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2015.02.023     Document Type: Article

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