Are serum-free and xeno-free culture conditions ideal for large scale clinical grade expansion of Wharton's jelly derived mesenchymal stem cells? A comparative study

Stem Cell Research and Therapy

Volumn 5, Issue 4, 2014, Pages

  Swamynathan, Priyanka ^a   Venugopal, Parvathy ^a   Kannan, Suresh ^a   Thej, Charan ^a   Kolkundar, Udaykumar ^a   Bhagwat, Swaroop ^a   Ta, Malancha ^b   Majumdar, Anish Sen ^a   Balasubramanian, Sudha ^a  

^a Stempeutics Research Pvt Ltd   (India)

^b INDIAN INSTITUTE OF SCIENCE EDUCATION AND RESEARCH KOLKATA   (India)

Author keywords

[No Author keywords available]

Indexed keywords

SCATTER FACTOR; TRANSFORMING GROWTH FACTOR BETA1; VASCULOTROPIN; CULTURE MEDIUM; SERUM-FREE MEDIUM; XENOBIOTIC AGENT;

ANGIOGENESIS; ANIMAL CELL; APOPTOSIS; ARTICLE; CELL CYCLE ASSAY; CELL DIFFERENTIATION; CELL EXPANSION; CELL ISOLATION; CELL KINETICS; CELL LINEAGE; CELL PROLIFERATION; CELL SIZE; CELL TRANSFORMATION; CLONE; COLONY FORMING UNIT; COMPARATIVE STUDY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CRYOPRESERVATION; CULTURE TECHNIQUE; ENZYME LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; GENETIC ANALYSIS; GENETIC STABILITY; GROWTH KINETICS; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOGENICITY; IMMUNOPHENOTYPING; IMMUNOSUPPRESSIVE TREATMENT; IN VITRO STUDY; INTERMETHOD COMPARISON; KARYOTYPE; LARGE SCALE PRODUCTION; MESENCHYMAL STROMA CELL; NONHUMAN; PROTEIN SECRETION; SECRETOME; SENESCENCE; SERUM FREE CULTURE; XENO FREE CULTURE; CELL AGING; CELL CULTURE TECHNIQUE; CELL CYCLE; CULTURE MEDIUM; CYTOLOGY; GENOMIC INSTABILITY; SERUM-FREE MEDIUM; WHARTON JELLY;

CELL AGING; CELL CULTURE TECHNIQUES; CELL CYCLE; CELL DIFFERENTIATION; CELL PROLIFERATION; CULTURE MEDIA; CULTURE MEDIA, SERUM-FREE; GENOMIC INSTABILITY; HUMANS; MESENCHYMAL STROMAL CELLS; WHARTON JELLY; XENOBIOTICS;

EID: 84904779876     PISSN: None     EISSN: 17576512     Source Type: Journal    
DOI: 10.1186/scrt477     Document Type: Article

References (31)

1. Mesenchymal stem cells. Caplan AI, J Orthop Res 1991 9 641 650
2. Cell surface antigens on human marrow-derived mesenchymal cells are detected by monoclonal antibodies. Haynesworth SE, Baber MA, Caplan AI, Bone 1992 13 69 80
3. Mesenchymal stem cells: revisiting history, concepts, and assays. Bianco P, Robey PG, Simmons PJ, Cell Stem Cell 2008 2 313 319
4. How do mesenchymal stromal cells exert their therapeutic benefit? Horwitz EM, Dominici M, Cytotherapy 2008 10 771 774
5. Heterotopic of bone marrow, analysis of precursor cells for osteogenic and hematopoietic tissues. Friedenstein AJ, Petrakova KV, Kurolesova AI, Frolova GP, Transplantation 1968 6 230 247
6. Mesenchymal stem cells derived from CD133-positive cells in mobilized peripheral blood and cord blood: proliferation, Oct4 expression, and plasticity. Tondreau T, Meuleman N, Delforge A, Dejeneffe M, Leroy R, Massy M, Mortier C, Bron D, Lagneaux L, Stem Cells 2005 23 1105 1112
7. Multilineage cells from human adipose tissue: implications for cell-based therapies. Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, Benhaim P, Lorenz HP, Hedrcik MH, Tissue Eng 2001 7 211 228
8. Identification of mesenchymal stem/progenitor cells in human first-trimester fetal blood, liver, and bone marrow. Campagnoli C, Roberts IA, Kumar S, Bennett PR, Bellantuono I, Fisk NM, Blood 2001 98 2396 2402
9. Mesenchymal stem cells from cryopreserved human umbilical cord blood. Lee MW, Choi J, Yang MS, Moon YJ, Park JS, Kim HC, Kim YJ, Biochem Biophys Res Commun 2004 320 273 278
10. Optimization and scale-up of Wharton's jelly-derived mesenchymal stem cells for clinical applications. Nekanti U, Mohanty L, Venugopal P, Balasubramanian S, Totey S, Ta M, Stem Cell Res 2010 5 244 254
11. Method to isolate mesenchymal-like cells from Wharton's jelly of umbilical cord. Seshareddy K, Troyer D, Weiss ML, Methods Cell Biol 2008 86 101 119
12. Wharton's jelly-derived cells are a primitive stromal cell population. Troyer DL, Weiss ML, Stem Cells 2008 26 591 599
13. From bone marrow to therapeutic applications: different behavior and genetic/epigenetic stability during mesenchymal stem cell expansion in autologous and foetal bovine sera. Tonti GA, Mannello F, Int J Dev Biol 2008 52 1023 1032
14. MSCs in hematopoietic cell transplantation. Horwitz EM, Maziarz RT, Kebriaei P, Biol Blood Marrow Transplant 2011 17 21 S29
15. Isolation method and xeno-free culture conditions influence multipotent differentiation capacity of human Wharton's jelly-derived mesenchymal stem cells. Corotchi MC, Popa MA, Remes A, Sima LE, Gussi I, Lupu Plesu M, Stem Cell Res Ther 2013 4 81
16. CD200 positive human mesenchymal stem cells suppress TNF-alpha secretion from CD200 receptor positive macrophage-like cells. Pietilä M, Lehtonen S, Tuovinen E, Lähteenmäki K, Laitinen S, Leskela HV, Natynki A, Pesala J, Nordstrom K, Lehenkari P, PLoS One 2012 7 31671
17. Limited acquisition of chromosomal abberations in human adult mesenchymal stromal cells. Sensebe L, Tarte K, Kampera M, Martin I, Phinney DG, Shi Y, MSC Committee of the Internation Society for Cellular Therapy, Cell Stem Cell 2012 10 9 10
18. Mesenchymal stem cells from human umbilical cord express preferentially secreted factors related to neuroprotection, neurogenesis, and angiogenesis. Hsieh JY, Wang HW, Chang SJ, Liao KH, Lee IH, Lin WS, Wu CH, Lin WY, Cheng SM, PLoS One 2013 8 72604
19. Mesenchymal stem cells for clinical application. Sensebé L, Krampera M, Schrezenmeier H, Bourin P, Giordano R, Vox Sang 2010 98 93 107
20. Development and characterization of a clinically compliant xeno-free culture medium in good manufacturing practice for human multipotent mesenchymal stem cells. Lucas LG, Yang S, Zachar V, Yang Z, Lakshmipathy U, Bradford J, Boucher SE, Vemuri MC, Stem Cells Transl Med 2012 1 750 758
21. Umbilical cord tissue-derived mesenchymal stem cells grow best under GMP-compliant culture conditions and maintain their phenotypic and functional properties. Hartmann I, Hollweck T, Haffner S, Krebs M, Meiser B, Reichart B, Eissner G, J Immunol Methods 2010 363 80 89
22. Serum-free culture: the serum-free media interactive online database. Brunner D, Frank J, Appl H, Schoffl H, Pfaller W, Gstraunthaler G, ALTEX 2010 27 53 62
23. Serum-free, xeno-free culture media maintain the proliferation rate and multipotentiality of adipose stem cells in vitro. Lindroos B, Boucher S, Chase L, Kuokkanen H, Huhtala H, Haataja R, Vemuri M, Suuronen R, Miettinen S, Cytotherapy 2009 11 958 972
24. Development of fully defined xeno-free culture system for the preparation and propagation of cell therapy-compliant human adipose stem cells. Patrikoski M, Juntunen M, Boucher S, Campbell A, Vemuri MC, Mannerström B, Miettinen S, Stem Cells Res Ther 2013 4 27
25. Novel immunomodulatory markers expressed by human WJ MSC: an updated review in regenerative and reparative medicine. Rocca GL, Corrao S, Iacono ML, Corsello T, Farina F, Anzalone R, Open Tissue Eng Regen Med J 2012 5 50 58
26. Suppression of allogeneic T-cell proliferation by human marrow stromal cells: implications in transplantation. Tse WT, Pendleton JD, Beyer WM, Egalka MC, Guinan EC, Transplantation 2003 75 389 397
27. Harnessing the mesenchymal stem cell secretome for the treatment of cardiovascular disease. Ranganath SH, Levy O, Inamdar MS, Karp JM, Cell Stem Cell 2012 10 244 258
28. Proangiogenic features of Wharton's jelly-derived mesenchymal stromal/stem cells and their ability to form functional vessels. Choi M, Lee NS, Naidansaren P, Kim HKOE, Strunk D, Koch C, Int J Biochem Cell Biol 2013 45 560 570
29. How to track cellular aging of mesenchymal stromal cells? Wagner W, Bork S, Lepperdinger G, Joussen S, Ma N, Strunk D, Koch C, Aging 2010 2 4
30. Different facets of aging in human mesenchymal stem cells. Wagner W, Ho AD, Zenke M, Tissue Eng Part B Rev 2010 16 445 453
31. Ex vivo expansion of human mesenchymal stem cells in defined serum-free media. Jung S, Panchalingam KM, Rosenberg L, Behie LA, Stem Cells Int 2012 1155 123030