Glycoengineering of Chinese hamster ovary cells for enhanced erythropoietin N-glycan branching and sialylation

Biotechnology and Bioengineering

Volumn 112, Issue 11, 2015, Pages 2343-2351

  Yin, Bojiao ^a   Gao, Yuan ^a   Chung, Cheng yu ^a   Yang, Shuang ^b   Blake, Emily ^a   Stuczynski, Mark C ^a   Tang, Juechun ^a   Kildegaard, Helene F ^c   Andersen, Mikael R ^d   Zhang, Hui ^b   Betenbaugh, Michael J ^a  

^a Johns Hopkins University   (United States)

^b JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c Chalmers University of Technology   (Denmark)

^d TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

CHO; Erythropoietin; GNTIV Mgat4; GNTV Mgat5; N glycosylation; Sialic acid

Indexed keywords

ANTENNAS; BIOACTIVITY; CARBOXYLIC ACIDS; CELL CULTURE; CELLS; COORDINATION REACTIONS; CYTOLOGY; ESTERIFICATION; GLYCOPROTEINS; GLYCOSYLATION; MASS SPECTROMETRY; MOBILE SECURITY; PROTEINS;

CHO; ERYTHROPOIETIN (EPO); GNTIV/MGAT4; GNTV/MGAT5; N-GLYCOSYLATION; SIALIC ACIDS;

POLYSACCHARIDES;

ALPHA 1,3 DEXTRO MANNOSIDE; ALPHA 1,6 DEXTRO MANNOSIDE BETA 1,6 N ACETYLGLUCOSAMINYLTRANSFERASE; BETA 1,4 N ACETYLGLUCOSAMINYLTRANSFERASE; CELL PROTEIN; ERYTHROPOIETIN; GLYCAN; GLYCAN DERIVATIVE; GLYCOPROTEIN; GLYCOSYLTRANSFERASE; LECTIN; MANNOSIDE; N ACETYLGLUCOSAMINYLTRANSFERASE; N GLYCAN; RECOMBINANT ERYTHROPOIETIN; SIALIC ACID; SUGAR; UNCLASSIFIED DRUG; URIDINE DIPHOSPHATE N ACETYLGLUCOSAMINE; POLYSACCHARIDE; PROTEIN BINDING; RECOMBINANT PROTEIN;

ANIMAL CELL; ARTICLE; CELL ENGINEERING; CHO CELL LINE; CONTROLLED STUDY; ENGINEERING; FEMALE; GLYCOENGINEERING; GLYCOSYLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; MASS SPECTROMETRY; NONHUMAN; PROTEIN EXPRESSION; SIALYLATION; WILD TYPE; ANIMAL; CRICETULUS; GENETICS; METABOLIC ENGINEERING; METABOLISM;

ANIMALS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CRICETULUS; ERYTHROPOIETIN; FEMALE; GLYCOSYLATION; GLYCOSYLTRANSFERASES; HUMANS; LECTINS; MASS SPECTROMETRY; METABOLIC ENGINEERING; POLYSACCHARIDES; PROTEIN BINDING; RECOMBINANT PROTEINS;

EID: 84942363838     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25650     Document Type: Article

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