Insulin-producing endocrine cells differentiated in vitro from human embryonic stem cells function in macroencapsulation devices in vivo

Stem Cells Translational Medicine

Volumn 4, Issue 10, 2015, Pages 1214-1222

  Agulnick, Alan D ^a   Ambruzs, Dana M ^a   Moorman, Mark A ^a   Bhoumik, Anindita ^a   Cesario, Rosemary M ^a   Payne, Janice K ^a   Kelly, Jonathan R ^a   Haakmeester, Carl ^a   Srijemac, Robert ^a   Wilson, Alistair Z ^a   Kerr, Justin ^a   Frazier, Mauro A ^a   Kroon, Evert J ^a   D’Amour, Kevin A ^a  

^a Georgia Biobusiness Center   (United States)

Author keywords

Cell therapy; Diabetes; Drug delivery systems; Embryonic stem cells; Insulin secreting cells; Pancreas development

Indexed keywords

ACTIVIN A; C PEPTIDE; GAMMA SECRETASE; GLUCAGON; INSULIN; NEU DIFFERENTIATION FACTOR; NEU DIFFERENTIATION FACTOR BETA1; NEUROGENIN 3; NOTCH RECEPTOR; PANCREAS SPECIFIC TRANSCRIPTION FACTOR 1A; PROINSULIN; RHO KINASE; SOMATOSTATIN; TRANSCRIPTION FACTOR NKX6.1; TRANSCRIPTION FACTOR PDX 1; TRANSCRIPTION FACTOR SOX9; UNCLASSIFIED DRUG; WNT3A PROTEIN; BIOLOGICAL MARKER; GLUCOSE BLOOD LEVEL; HOMEODOMAIN PROTEIN; NKX6-1 PROTEIN, HUMAN; PANCREATIC AND DUODENAL HOMEOBOX 1 PROTEIN; TRANSACTIVATOR PROTEIN;

ARTICLE; CELL AGGREGATION; CELL DIFFERENTIATION; CELL INTERACTION; CELL MATURATION; CONTROLLED STUDY; EMBRYONIC STEM CELL; ENCAPSULATION; ENDOCRINE CELL; ENZYME LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; GENE EXPRESSION; GENERAL DEVICE; HISTOLOGY; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOFLUORESCENCE TEST; IN VIVO STUDY; INSULIN RELEASE; MACROENCAPSULATION; MACROENCAPSULATION DEVICE; PANCREAS ISLET BETA CELL; RNA ANALYSIS; SURVIVAL; ANALYSIS; ANIMAL; BIOSYNTHESIS; CELL CULTURE; CELL SEPARATION; CRYOPRESERVATION; CYTOLOGY; GENE EXPRESSION PROFILING; GLUCOSE BLOOD LEVEL; HUMAN EMBRYONIC STEM CELL; IMMOBILIZED CELL; METABOLISM; MOUSE; PROCEDURES; PROTEIN PROCESSING; REPRODUCIBILITY; SECRETION (PROCESS); TRANSPLANTATION;

ANIMALS; BIOMARKERS; BLOOD GLUCOSE; CELL DIFFERENTIATION; CELL SEPARATION; CELLS, CULTURED; CELLS, IMMOBILIZED; CRYOPRESERVATION; GENE EXPRESSION PROFILING; HOMEODOMAIN PROTEINS; HUMAN EMBRYONIC STEM CELLS; HUMANS; INSULIN; INSULIN-SECRETING CELLS; MICE; PROINSULIN; PROTEIN PROCESSING, POST-TRANSLATIONAL; REPRODUCIBILITY OF RESULTS; TRANS-ACTIVATORS;

EID: 84942120540     PISSN: 21576564     EISSN: 21576580     Source Type: Journal    
DOI: 10.5966/sctm.2015-0079     Document Type: Article

References (27)

1. McCall M, Shapiro AM. Update on islet transplantation. Cold Spring Harb Perspect Med 2012;2:a007823.
2. Pagliuca FW, Melton DA. How to make a functional b-cell. Development 2013;140: 2472-2483.
3. Kelly OG, Chan MY, Martinson LA et al. Cell-surface markers for the isolation of pancreatic cell types derived fromhuman embryonic stem cells. Nat Biotechnol 2011;29: 750-756.
4. Kroon E, Martinson LA, Kadoya K et al. Pancreatic endoderm derived from human embryonic stem cells generates glucose-responsive insulin-secreting cells in vivo. Nat Biotechnol 2008;26:443-452.
5. Rezania A, Bruin JE, Riedel MJ et al. Maturation of human embryonic stem cell-derived pancreatic progenitors into functional islets capable of treating pre-existing diabetes in mice. Diabetes 2012;61:2016-2029.
6. Schulz TC, Young HY, Agulnick AD et al. A scalable system for production of functional pancreatic progenitors from human embryonic stem cells. PLoS One 2012;7:e37004.
7. Bruin JE, Rezania A, Xu J et al. Maturation and function of human embryonic stem cellderived pancreatic progenitors in macroencapsulation devices following transplant into mice. Diabetologia 2013;56:1987-1998.
8. Kirk K, Hao E, Lahmy R et al. Human embryonic stem cell derived islet progenitors mature inside an encapsulation device without evidence of increased biomass or cell escape. Stem Cell Res (Amst) 2014;12:807-814.
9. Basford CL, Prentice KJ, Hardy AB et al. The functional and molecular characterisation of human embryonic stemcell-derived insulinpositive cells compared with adult pancreatic beta cells. Diabetologia 2012;55:358-371.
10. Bruin JE, Erener S, Vela J et al. Characterization of polyhormonal insulin-producing cells derived in vitro from human embryonic stem cells. Stem Cell Res (Amst) 2014;12:194-208.
11. Hrvatin S, O'Donnell CW, Deng F et al. Differentiated human stem cells resemble fetal, not adult, b cells. Proc Natl Acad Sci USA 2014;111:3038-3043.
12. Xie R, Everett LJ, Lim HW et al. Dynamic chromatin remodeling mediated by polycomb proteins orchestrates pancreatic differentiation of human embryonic stem cells. Cell Stem Cell 2013;12:224-237.
13. Pagliuca FW, Millman JR, Gürtler M et al. Generation of functional human pancreatic b cells in vitro. Cell 2014;159:428-439.
14. Rezania A, Bruin JE, Arora P et al. Reversal of diabetes with insulin-producing cells derived in vitro from human pluripotent stem cells. Nat Biotechnol 2014;32:1121-1133.
15. Rukstalis JM, Habener JF. Neurogenin3: A master regulator of pancreatic islet differentiation and regeneration. Islets 2009;1: 177-184.
16. Afelik S, Jensen J. Notch signaling in the pancreas: Patterning and cell fate specification. Wiley Interdiscip Rev Dev Biol 2013;2: 531-544.
17. Jorissen E, De Strooper B. Gammasecretase and the intramembrane proteolysis of Notch. Curr Top Dev Biol 2010;92:201-230.
18. Huynh C, Poliseno L, Segura MF et al. The novel gamma secretase inhibitor RO4929097 reduces the tumor initiating potential of melanoma. PLoS One 2011;6:e25264.
19. Aguayo-Mazzucato C, Zavacki AM, Marinelarena A et al. Thyroid hormone promotes postnatal rat pancreatic b-cell development and glucose-responsive insulin secretion through MAFA. Diabetes 2013;62: 1569-1580.
20. Otonkoski T, Beattie GM, Mally MI et al. Nicotinamide is a potent inducer of endocrine differentiation in cultured human fetal pancreatic cells. J Clin Invest 1993;92: 1459-1466.
21. Olson MF. Applications for ROCK kinase inhibition. Curr Opin Cell Biol 2008;20: 242-248.
22. Watanabe K, Ueno M, Kamiya D et al. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotechnol 2007;25:681-686.
23. D'Amour KA, Bang AG, Eliazer S et al. Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells. Nat Biotechnol 2006;24:1392-1401.
24. Spijker HS, Ravelli RB, Mommaas-Kienhuis AM et al. Conversion of mature human b-cells into glucagon-producing a-cells. Diabetes 2013;62:2471-2480.
25. Murtaugh LC. The what, where, when and how of Wnt/b-catenin signaling in pancreas development. Organogenesis 2008;4: 81-86.
26. Wiater E, Vale W. Roles of activin family in pancreatic development and homeostasis. Mol Cell Endocrinol 2012;359:23-29.
27. Johansson KA, Dursun U, Jordan N et al. Temporal control of neurogenin3 activity in pancreas progenitors reveals competence windows for the generation of different endocrine cell types. Dev Cell 2007;12:457-465.