Development of a fluorescence-activated cell sorting method coupled with whole genome amplification to analyze minority and trace dehalococcoides genomes in microbial communities

Environmental Science and Technology

Volumn 49, Issue 3, 2015, Pages 1585-1593

  Lee, Patrick K H ^a,b   Men, Yujie ^a,e   Wang, Shanquan ^c,f   He, Jianzhong ^c   Alvarez Cohen, Lisa ^a,d  

^a 109 McLaughlin Hall   (United States)

^b CITY UNIVERSITY OF HONG KONG   (Hong Kong)

^c NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^d LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

^e SWISS FEDERAL INSTITUTE OF AQUATIC SCIENCE AND TECHNOLOGY   (Switzerland)

^f United States of America   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; BIOASSAY; DECHLORINATION; FLOW CYTOMETRY; FLUORESCENCE; GENES; QUALITY CONTROL;

COMPARATIVE GENOMICS; FLUORESCENCE ACTIVATED CELL SORTING; GENOMIC CHARACTERIZATION; INTRA-SAMPLE VARIABILITY; MICROBIAL COMMUNITIES; REDUCTIVE DECHLORINATION; RELATIVE CONCENTRATION; WHOLE GENOME AMPLIFICATIONS;

CYTOLOGY;

DNA; GENOMIC DNA;

CATALYSIS; CHLORINATION; CONCENTRATION (COMPOSITION); DNA; FLUORESCENCE; GENOME; GERM CELL; MICROBIAL ACTIVITY; MICROBIAL COMMUNITY; SORPTION;

ANAEROBIC BACTERIUM; ANIMAL CELL; ARTICLE; BACTERIAL CELL; BACTERIAL GENOME; BACTERIAL STRAIN; CELL ISOLATION; COMPARATIVE GENOMIC HYBRIDIZATION; CONTROLLED STUDY; DEHALOCOCCOIDES; DEHALOCOCCOIDES MCCARTYI; ENRICHMENT CULTURE; ENVIRONMENT; FLUORESCENCE ACTIVATED CELL SORTING; FLUORESCENCE IN SITU HYBRIDIZATION; GENE AMPLIFICATION; GENE SEQUENCE; GENETIC ANALYSIS; GENETIC VARIABILITY; MICROARRAY ANALYSIS; MICROBIAL COMMUNITY; NONHUMAN; NUCLEOTIDE SEQUENCE; WHOLE GENOME AMPLIFICATION; CHLOROFLEXI; CYTOLOGY; DNA MICROARRAY; FLOW CYTOMETRY; GENETICS; GENOMICS; MICROBIAL CONSORTIUM; MOLECULAR GENETICS; PROCEDURES;

DEHALOCOCCOIDES;

CHLOROFLEXI; FLOW CYTOMETRY; GENOME, BACTERIAL; GENOMICS; IN SITU HYBRIDIZATION, FLUORESCENCE; MICROBIAL CONSORTIA; MOLECULAR SEQUENCE DATA; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS;

EID: 84942077411     PISSN: 0013936X     EISSN: 15205851     Source Type: Journal    
DOI: 10.1021/es503888y     Document Type: Article

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