Expression and purification of active recombinant human bone morphogenetic 7-2 dimer fusion protein

Protein Expression and Purification

Volumn 115, Issue , 2015, Pages 61-68

  Dang, Jianli ^a   Jing, Lei ^a   Shi, Weiwei ^a   Qin, Ping ^a   Li, Yuyin ^a   Diao, Aipo ^a  

^a TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Dialysis; Inclusion body; Osteogenesis; Protein refolding; rhBMP7 2

Indexed keywords

BMP7 PROTEIN, HUMAN; HYBRID PROTEIN; OSTEOGENIC PROTEIN 1;

AMINO ACID SEQUENCE; ANIMAL; BONE DEVELOPMENT; CELL INCLUSION; CELL LINE; DRUG EFFECTS; FEMALE; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; MOUSE; OSTEOBLAST; RAT; SPRAGUE DAWLEY RAT;

AMINO ACID SEQUENCE; ANIMALS; BONE MORPHOGENETIC PROTEIN 7; CELL LINE; FEMALE; INCLUSION BODIES; MICE; MOLECULAR SEQUENCE DATA; OSTEOBLASTS; OSTEOGENESIS; RATS; RATS, SPRAGUE-DAWLEY; RECOMBINANT FUSION PROTEINS;

EID: 84941938697     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2015.08.010     Document Type: Article

References (23)

1. A.H. Reddi, and W.A. Anderson Collagenous bone matrix-induced endochondral ossification hemopoiesis J. Cell Biol. 69 1976 557 572
2. J.M. Wozney, V. Rosen, A.J. Celeste, L.M. Mitsock, M.J. Whitters, R.W. Kriz, R.M. Hewick, and E.A. Wang Novel regulators of bone formation: molecular clones and activities J. Sci. 242 1988 1528 1534
3. D.Y. Suh, S.D. Boden, J. Louis-Ugbo, M. Mayr, H. Murakami, H.S. Kim, A. Minamide, and W.C. Hutton Delivery of recombinant human bone morphogenetic protein-2 using a compression-resistant matrix in posterolateral spine fusion in the rabbit and in the non-human primate J. Spine. 27 2002 353 360
4. T. Akamaru, D. Suh, S.D. Boden, H.S. Kim, and A. Minamide Simple carrier matrix modifications can enhance delivery of recombinant human bone morphogenetic protein-2 for posterolateral spine fusion J. Spine. 28 2003 429 434
5. L. Nissinen, L. Pirilä, and J. Heino Bone morphogenetic protein-2 is a regulator of cell adhesion Exp. Cell Res. 230 1997 377 385
6. S. Ebara, and K. Nakayama Mechanism for the action of bone morphogenetic proteins and regulation of their activity J. Spine. 27 2002 S10 S15
7. C.A. Kirker-Head Potential applications and delivery strategies for bone morphogenetic proteins Adv. Drug Deliv. Rev. 43 2009 65 92
8. M. Zeisberg, J. Hanai, H. Sugimoto, T. Mammoto, D. Charytan, F. Strutz, and R. Kalluri BMP-7 counteracts TGF-beta1-induced epithelial-to-mesenchymal transition and reverses chronic renal injury Nat. Med. 9 2003 964 968
9. M. Zeisberg, A. Shah, and R. Kalluri Bone morphogenic protein-7 induces mesenchymal to epithelial transition in adult renal fibroblasts and facilitates regeneration of injured kidney J. Biol. Chem. 280 2005 8094 8100
10. K. Bessho, K. Kusumoto, K. Fujimura, Y. Konishi, and Y. Ogawa Comparison of recombinant and purified human bone morphogenetic protein Br. J. Oral Maxillofac. Surg. 37 1999 2 5
11. D.I. Israel, J. Nove, K.M. Kerns, I.K. Moutsatsos, and R.J. Kaufman Expression and characterization of bone morphogenetic protein-2 in Chinese hamster ovary cells J. Growth Factors 7 1992 139 150
12. G. Suo, B. Chen, J. Zhang, Y. Gao, X. Wang, Z. He, and J. Dai Expression of active hBMP2 in transgenic tobacco plants J. Plant Cell Rep. 12 2006 1316 1324
13. N. Ishida, M. Tsujimoto, and T. Kanaya Expression and characterization of hBMP2 in silkworm larbae infected with recombinant BmNPV J. Biochem. 115 1994 279
14. T.K. Sampath, J.E. Coughlin, R.M. Whetstone, D. Banach, C. Corbett, and R.J. Ridge Bovine osteogenic protein is composed of dimers of OP-1 and BMP-2A, two members of the transforming growth factor-beta superfamily J. Biol. Chem. 265 1990 13198 13205
15. E. Ozkaynak, D.C. Rueger, E.A. Drier, C. Corbett, R.J. Ridge, T.K. Sampath, and H. Oppermann OP-1 cDNA encodes an osteogenic protein in the TGF-beta family J. EMBO 9 1990 2085 2093
16. K.M. Lyons, B.L. Hogan, and E.J. Robertson Colocalization of BMP 7 and BMP 2 RNAs suggests that these factors cooperatively mediate tissue interactions during murine development J. Mech. Dev. 50 1995 71 83
17. W. Zhu, B.A. Rawlins, O.B. Adjei, E.R. Myers, J. Arimizu, E. Choi, J.R. Lieberman, R.G. Crystal, and C. Hidaka Combined bone morphogenetic protein-2 and -7 gene transfer enhances osteoblastic differentiation and spine fusion in a rodent model J. JBMR 19 2004 2021 2032
18. L.F. Vallejo, M. Brokelmann, S. Marten, S. Trappe, J. Cabrera-Crespo, A. Hoffmann, G. Gross, H.A. Weich, and U. Rinas Renaturation and purification of bone morphogenetic protein-2 produced as inclusion bodies in high-cell-density cultures of recombinant Escherichia coli J. Biotechnol. 94 2002 185 194
19. C. Scheufler, W. Sebald, and M. Hülsmeyer Crystal structure of human bone morphogenetic protein-2 at 2.7 A resolution J. Mol. Biol. 287 1999 103 115
20. W. Zhu, J. Kim, C. Cheng, B.A. Rawlins, O.B. Adjei, R.G. Crystal, and C. Hidaka Noggin regulation of bone morphogenetic protein (BMP) 2/7 heterodimer activity in vitro J. Bone 39 2006 61 71
21. L.F. Vallejo, M. Brokelmann, S. Marten, and et al. Renaturation and purification of bone morphogenetic protein-2 produced as inclusion bodies in high-cell-density cultures of recombinant Escherichia coli J. Biotechnol. 94 2002 185 194
22. T.M. Kilian, and A.G. Beck-Sickinger Recombinant expression and characterization of biologically active protein delta homolog 1 J. Protein Exp. Purif. 110 2015 72 78
23. W. Guo, X.C. Zhu, J. Cai, and et al. Refolding and two-step purification by hydrophobic interaction chromatography of recombinant human bone morphogenetic protein-2 from Escherichia coli J. Process Biochem. 47 2012 960 967