Refolding and two-step purification by hydrophobic interaction chromatography of recombinant human bone morphogenetic protein-2 from Escherichia coli

Process Biochemistry

Volumn 47, Issue 6, 2012, Pages 960-967

  Guo, Wangming ^a,b   Zhu, Xiangcheng ^a   Cai, Jin ^a   Huang, Lei ^a   Cen, Peilin ^a   Xu, Zhinan ^a  

^a ZHEJIANG UNIVERSITY   (China)

^b Hangzhou Jiuyuan Gene Engineering Co Ltd   (China)

Author keywords

Hydrophobic interaction chromatography (HIC); N,N dimethylformamide (DMF); Purification; Recombinant human bone morphogenetic protein 2 (rhBMP 2); Refolding

Indexed keywords

CHINESE HAMSTER OVARY CELLS; HIGH PURITY; HOMODIMERS; HUMAN BONES; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; IN-VITRO; INCLUSION BODIES; N ,N-DIMETHYLFORMAMIDE; PURIFICATION PROCEDURES; RECOMBINANT HUMAN BONE MORPHOGENETIC PROTEIN-2 (RHBMP-2); REFOLDING; REFOLDING PROCESS; RHBMP-2; SALT CONTENT; WET WEIGHT;

DIMETHYLFORMAMIDE; ESCHERICHIA COLI; LIQUID CHROMATOGRAPHY; OPTIMIZATION; ORGANIC SOLVENTS; SEPARATION;

PURIFICATION;

CRICETULUS GRISEUS; ESCHERICHIA COLI;

EID: 84860375816     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2012.03.003     Document Type: Article

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