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For an example, see: http://www.activemotif.com/catalog/630/atto-sted-secondary-antibody-conjugates.
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The dye KK114L (previously reported as KK1119; see ref. [5d]) has the same fluorophore as KK114 (see refs. [5d, e], except the linker with a carboxylic acid residue is elongated by one CH2 group. This provides a much more stable NHS ester (see ref. [5c]), although the spectral properties remain exactly the same. Abberior STAR REDis an NHS carbonate with the same fluorophore as that in KK114 (for the structure, see Scheme 1 and ref. [5d]). Under STED conditions, these two rhodamines are more photostable than SiR-CO2H or SiRF dyes, despite having additional conjugated double bonds (as seen in Scheme 1). The latter may undergo photoinduced reactions that cause partial bleaching (so-called "bluing", that is, hypsochromic and hypsofluoric shifts). No less importantly, derivatives of KK114 are far less sensitive to changes in the excitation/depletion conditions and other setup settings and develop far less re-excitation artifacts
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The dye KK114L (previously reported as KK1119; see ref. [5d]) has the same fluorophore as KK114 (see refs. [5d, e], except the linker with a carboxylic acid residue is elongated by one CH2 group. This provides a much more stable NHS ester (see ref. [5c]), although the spectral properties remain exactly the same. Abberior STAR RED (www.abberior.com) is an NHS carbonate with the same fluorophore as that in KK114 (for the structure, see Scheme 1 and ref. [5d]). Under STED conditions, these two rhodamines are more photostable than SiR-CO2H or SiRF dyes, despite having additional conjugated double bonds (as seen in Scheme 1). The latter may undergo photoinduced reactions that cause partial bleaching (so-called "bluing", that is, hypsochromic and hypsofluoric shifts). No less importantly, derivatives of KK114 are far less sensitive to changes in the excitation/depletion conditions and other setup settings and develop far less re-excitation artifacts.
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