Engineering the carbohydrate-binding site of Epa1p from Candida glabrata: Generation of adhesin mutants with different carbohydrate specificity

Glycobiology

Volumn 24, Issue 12, 2014, Pages 1312-1322

  Ielasi, Francesco S ^a   Verhaeghe, Tom ^b   Desmet, Tom ^b   Willaert, Ronnie G ^a  

^a VRIJE UNIVERSITEIT BRUSSEL   (Belgium)

^b GHENT UNIVERSITY   (Belgium)

Author keywords

Candida glabrata; Epa1; Epithelial adhesin; Glycan array; Saturation mutagenesis

Indexed keywords

ADHESIN; CARBOHYDRATE; EPA1P PROTEIN; FUNGAL PROTEIN; GLYCAN; LECTIN; UNCLASSIFIED DRUG; EPITHELIAL ADHESIN 1, CANDIDA GLABRATA;

ARTICLE; BINDING SITE; CANDIDA GLABRATA; COMPARATIVE STUDY; CONTROLLED STUDY; CRYSTALLOGRAPHY; DISSOCIATION CONSTANT; HUMAN; HUMAN CELL; LIGAND BINDING; MOLECULAR DOCKING; MOLECULAR PROBE; MUTAGENESIS; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN ENGINEERING; PROTEIN EXPRESSION; SURFACE PLASMON RESONANCE; CHEMISTRY; ENZYME SPECIFICITY; GENETICS; METABOLISM; MUTATION;

BINDING SITES; CARBOHYDRATES; FUNGAL PROTEINS; LECTINS; MUTATION; PROTEIN ENGINEERING; SUBSTRATE SPECIFICITY;

EID: 84940753705     PISSN: 09596658     EISSN: 14602423     Source Type: Journal    
DOI: 10.1093/glycob/cwu075     Document Type: Article

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