Lycopene reduces cholesterol absorption through the downregulation of Niemann-Pick C1-like 1 in Caco-2 cells

Molecular Nutrition and Food Research

Volumn 59, Issue 11, 2015, Pages 2225-2230

  Zou, Jun ^a   Feng, Dan ^b  

^a The Second People s Hospital of Nanhai District   (China)

^b SUN YAT SEN UNIVERSITY   (China)

Author keywords

Caco 2 cells; Cholesterol; Liver X receptor ; Lycopene; NPC1L1 protein

Indexed keywords

CAROTENOID; CHOLESTEROL; LIVER X RECEPTOR; LYCOPENE; MEMBRANE PROTEIN; NPC1L1 PROTEIN, HUMAN;

ANTAGONISTS AND INHIBITORS; CACO-2 CELL LINE; DOWN REGULATION; DRUG EFFECTS; HUMAN; INTESTINE ABSORPTION; METABOLISM; PHYSIOLOGY; SIGNAL TRANSDUCTION;

CACO-2 CELLS; CAROTENOIDS; CHOLESTEROL; DOWN-REGULATION; HUMANS; INTESTINAL ABSORPTION; LIVER X RECEPTORS; MEMBRANE PROTEINS; SIGNAL TRANSDUCTION;

EID: 84940676345     PISSN: 16134125     EISSN: 16134133     Source Type: Journal    
DOI: 10.1002/mnfr.201500221     Document Type: Article

References (35)

1. Weingartner, O., Lutjohann, D., Bohm, M., Laufs, U., Cholesterol homeostasis and cardiovascular risk. Dtsch. Med. Wochenschr. 2011, 136, 34-38.
2. Kruit, J. K., Groen, A. K., van Berkel, T. J., Kuipers, F., Emerging roles of the intestine in control of cholesterol metabolism. World J. Gastroenterol. 2006, 12, 6429-6439.
3. Gylling, H., Miettinen, T. A., The effect of cholesterol absorption inhibition on low density lipoprotein cholesterol level. Atherosclerosis 1995, 117, 305-308.
4. Altmann, S. W., Davis, H. R., Jr., Zhu, L. J., Yao, X. et al., Niemann-Pick C1 Like 1 protein is critical for intestinal cholesterol absorption. Science 2004, 303, 1201-1204.
5. Davis, H. R., Jr., Hoos, L. M., Tetzloff, G., Maguire, M. et al., Deficiency of Niemann-Pick C1 Like 1 prevents atherosclerosis in ApoE-/- mice. Arterioscler. Thromb. Vasc. Biol. 2007, 27, 841-849.
6. Davis, H. R., Jr., Zhu, L. J., Hoos, L. M., Tetzloff, G. et al., Niemann-Pick C1 Like 1 (NPC1L1) is the intestinal phytosterol and cholesterol transporter and a key modulator of whole-body cholesterol homeostasis. J. Biol. Chem. 2004, 279, 33586-33592.
7. Duval, C., Touche, V., Tailleux, A., Fruchart, J. C. et al., Niemann-Pick C1 like 1 gene expression is down-regulated by LXR activators in the intestine. Biochem. Biophys. Res. Commun. 2006, 340, 1259-1263.
8. Zhao, C., Dahlman-Wright, K., Liver X receptor in cholesterol metabolism. J. Endocrinol. 2010, 204, 233-240.
9. Fievet, C., Staels, B., Liver X receptor modulators: effects on lipid metabolism and potential use in the treatment of atherosclerosis. Biochem. Pharmacol. 2009, 77, 1316-1327.
10. Krinsky, N. I., Johnson, E. J., Carotenoid actions and their relation to health and disease. Mol. Aspects Med. 2005, 26, 459-516.
11. Allen, C. M., Schwartz, S. J., Craft, N. E., Giovannucci, E. L. et al., Changes in plasma and oral mucosal lycopene isomer concentrations in healthy adults consuming standard servings of processed tomato products. Nutr. Cancer 2003, 47, 48-56.
12. Di Mascio, P., Kaiser, S., Sies, H., Lycopene as the most efficient biological carotenoid singlet oxygen quencher. Arch. Biochem. Biophys. 1989, 274, 532-538.
13. Dahan, K., Fennal, M., Kumar, N. B., Lycopene in the prevention of prostate cancer. J. Soc. Integr. Oncol. 2008, 6, 29-36.
14. Feng, D., Ling, W. H., Duan, R. D., Lycopene suppresses LPS-induced NO and IL-6 production by inhibiting the activation of ERK, p38MAPK, and NF-kappaB in macrophages. Inflamm. Res. 2010, 59, 115-121.
15. Zou, J., Feng, D., Ling, W. H., Duan, R. D., Lycopene suppresses proinflammatory response in lipopolysaccharide-stimulated macrophages by inhibiting ROS-induced trafficking of TLR4 to lipid raft-like domains. J. Nutr. Biochem. 2013, 24, 1117-1122.
16. Hu, M. Y., Li, Y. L., Jiang, C. H., Liu, Z. Q. et al., Comparison of lycopene and fluvastatin effects on atherosclerosis induced by a high-fat diet in rabbits. Nutrition 2008, 24, 1030-1038.
17. Lorenz, M., Fechner, M., Kalkowski, J., Frohlich, K. et al., Effects of lycopene on the initial state of atherosclerosis in New Zealand White (NZW) rabbits. PloS One 2012, 7, e30808.
18. Kuhad, A., Sharma, S., Chopra, K., Lycopene attenuates thermal hyperalgesia in a diabetic mouse model of neuropathic pain. Eur. J. Pain 2008, 12, 624-632.
19. Navarro-Gonzalez, I., Perez-Sanchez, H., Martin-Pozuelo, G., Garcia-Alonso, J. et al., The inhibitory effects of bioactive compounds of tomato juice binding to hepatic HMGCR: in vivo study and molecular modelling. PloS One 2014, 9, e83968.
20. Misra, R., Mangi, S., Joshi, S., Mittal, S. et al., LycoRed as an alternative to hormone replacement therapy in lowering serum lipids and oxidative stress markers: a randomized controlled clinical trial. J. Obstet. Gynaecol. Res. 2006, 32, 299-304.
21. Silaste, M. L., Alfthan, G., Aro, A., Kesaniemi, Y. A. et al., Tomato juice decreases LDL cholesterol levels and increases LDL resistance to oxidation. Br. J. Nutr. 2007, 98, 1251-1258.
22. Ried, K., Fakler, P., Protective effect of lycopene on serum cholesterol and blood pressure: Meta-analyses of intervention trials. Maturitas 2011, 68, 299-310.
23. Palozza, P., Simone, R., Catalano, A., Parrone, N. et al., Lycopene regulation of cholesterol synthesis and efflux in human macrophages. J. Nutr. Biochem. 2011, 22, 971-978.
24. Fuhrman, B., Elis, A., Aviram, M., Hypocholesterolemic effect of lycopene and beta-carotene is related to suppression of cholesterol synthesis and augmentation of LDL receptor activity in macrophages. Biochem. Biophys. Res. Commun. 1997, 233, 658-662.
25. Gibson, K. M., Hoffmann, G., Schwall, A., Broock, R. L. et al., 3-Hydroxy-3-methylglutaryl coenzyme A reductase activity in cultured fibroblasts from patients with mevalonate kinase deficiency: differential response to lipid supplied by fetal bovine serum in tissue culture medium. J. Lipid Res. 1990, 31, 515-521.
26. Feng, D., Ohlsson, L., Duan, R. D., Curcumin inhibits cholesterol uptake in Caco-2 cells by down-regulation of NPC1L1 expression. Lipids Health Dis. 2010, 9, 40.
27. Field, F. J., Shreves, T., Fujiwara, D., Murthy, S. et al., Regulation of gene expression and synthesis and degradation of 3-hydroxy-3-methylglutaryl coenzyme A reductase by micellar cholesterolin CaCo-2 cells. J. Lipid Res. 1991, 32, 1811-1821.
28. Eckhardt, E. R., Wang, D. Q., Donovan, J. M., Carey, M. C., Dietary sphingomyelin suppresses intestinal cholesterol absorption by decreasing thermodynamic activity of cholesterol monomers. Gastroenterology 2002, 122, 948-956.
29. Duan, R. D., Bergman, T., Xu, N., Wu, J. et al., Identification of human intestinal alkaline sphingomyelinase as a novel ecto-enzyme related to the nucleotide phosphodiesterase family. J. Biol. Chem. 2003, 278, 38528-38536.
30. Garcia-Calvo, M., Lisnock, J., Bull, H. G., Hawes, B. E. et al., The target of ezetimibe is Niemann-Pick C1-Like 1 (NPC1L1). Proc. Natl. Acad. Sci. USA 2005, 102, 8132-8137.
31. Jesch, E. D., Seo, J. M., Carr, T. P., Lee, J. Y., Sitosterol reduces messenger RNA and protein expression levels of Niemann-Pick C1-like 1 in FHs 74 Int cells. Nutr. Res. 2009, 29, 859-866.
32. Alvaro, A., Rosales, R., Masana, L., Vallve, J. C., Polyunsaturated fatty acids down-regulate in vitro expression of the key intestinal cholesterol absorption protein NPC1L1: no effect of monounsaturated nor saturated fatty acids. J. Nutr. Biochem. 2010, 21, 518-525.
33. Huang, Y., Zheng, Y., The probiotic Lactobacillus acidophilus reduces cholesterol absorption through the down-regulation of Niemann-Pick C1-like 1 in Caco-2 cells. Br. J. Nutr. 2010, 103, 473-478.
34. Boileau, T. W., Boileau, A. C., Erdman, J. W., Jr., Bioavailability of all-trans and cis-isomers of lycopene. Exp. Biol. Med. 2002, 227, 914-919.
35. Wang, X. D., Lycopene metabolism and its biological significance. Am. J. Clin. Nutr. 2012, 96, 1214S-1222S.