Immune cell subsets and their gene expression profiles from human PBMC isolated by Vacutainer Cell Preparation Tube (CPT™) and standard density gradient

BMC Immunology

Volumn 16, Issue 1, 2015, Pages

  Corkum, Christopher P ^a   Ings, Danielle P ^a   Burgess, Christopher ^b   Karwowska, Sylwia ^b   Kroll, Werner ^b,c   Michalak, Tomasz I ^a  

^a MEMORIAL UNIVERSITY OF NEWFOUNDLAND   (Canada)

^b Novartis Oncology Companion Diagnostics   (United States)

^c Quidel Corporation   (United States)

Author keywords

Affymetrix microarray; BD Vacutainer cell preparation tube; DNA; Ficoll density radient; Gene expression analysis; Immune cell subsets; Peripheral blood mononuclear cells; RNA

Indexed keywords

CD14 ANTIGEN; CD19 ANTIGEN; CD4 ANTIGEN; CD8 ANTIGEN; DNA; FICOLL; RNA;

ADULT; ARTICLE; B LYMPHOCYTE; BLOOD COLLECTION TUBE; CD4+ T LYMPHOCYTE; CD8+ T LYMPHOCYTE; CELL ISOLATION; CELL SUBPOPULATION; CELL VIABILITY; CONCENTRATION (PARAMETERS); CYTOTOXIC T LYMPHOCYTE; DENSITY GRADIENT CENTRIFUGATION; DNA EXTRACTION; GENE EXPRESSION PROFILING; HELPER CELL; HUMAN; HUMAN CELL; IMMUNOCOMPETENT CELL; INTERMETHOD COMPARISON; MICROARRAY ANALYSIS; MONOCYTE; NORMAL HUMAN; NUCLEIC ACID ANALYSIS; PERIPHERAL BLOOD MONONUCLEAR CELL; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; RNA EXTRACTION; CELL SEPARATION; CELL SURVIVAL; CRYOPRESERVATION; DEVICES; DNA MICROARRAY; ISOLATION AND PURIFICATION; LYMPHOCYTE SUBPOPULATION; METABOLISM; PRINCIPAL COMPONENT ANALYSIS; PROCEDURES;

ADULT; CELL SEPARATION; CELL SURVIVAL; CENTRIFUGATION, DENSITY GRADIENT; CRYOPRESERVATION; DNA; FICOLL; GENE EXPRESSION PROFILING; HUMANS; LYMPHOCYTE SUBSETS; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PRINCIPAL COMPONENT ANALYSIS; RNA;

EID: 84940191527     PISSN: None     EISSN: 14712172     Source Type: Journal    
DOI: 10.1186/s12865-015-0113-0     Document Type: Article

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