Comparison of four digital PCR platforms for accurate quantification of DNA copy number of a certified plasmid DNA reference material

Scientific Reports

Volumn 5, Issue , 2015, Pages

  Dong, Lianhua ^a   Meng, Ying ^b   Sui, Zhiwei ^a   Wang, Jing ^a   Wu, Liqing ^a   Fu, Boqiang ^a  

^a NATIONAL INSTITUTE OF METROLOGY   (China)

^b Hubei Institute of Measurement and Testing Technology ^*  (China)

Author keywords

[No Author keywords available]

Indexed keywords

CHINA; COMPARATIVE STUDY; COPY NUMBER VARIATION; DEVICE FAILURE ANALYSIS; DEVICES; EQUIPMENT DESIGN; EVALUATION STUDY; GENETICS; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLASMID; POLYMERASE CHAIN REACTION; PROCEDURES; REFERENCE VALUE; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; STANDARDS; VALIDATION STUDY;

BASE SEQUENCE; CHINA; DNA COPY NUMBER VARIATIONS; EQUIPMENT DESIGN; EQUIPMENT FAILURE ANALYSIS; MOLECULAR SEQUENCE DATA; PLASMIDS; POLYMERASE CHAIN REACTION; REFERENCE VALUES; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

EID: 84940056588     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep13174     Document Type: Article

References (18)

1. Bhat, S., Herrmann, J., Armishaw, P., Corbisier, P. & Emslie, K. R. Single molecule detection in nanofluidic digital array enables accurate measurement of DNA copy number. Anal. Bioanal. Chem. 394, 457-467 (2009).
2. Bhat, S. et al. Comparison of methods for accurate quantification of DNA mass concentration with traceability to the international system of units. Anal. Chem. 82, 7185-7192 (2010).
3. Sykes, P. J. et al. Quantitation of targets for PCR by use of limiting dilution. Biotech. 13, 444-449 (1992).
4. Vogelstein, B. & Kinzler, K. Digital PCR. Proc. Natl. Acad. Sci. USA 96, 9236-9241 (1999).
5. Sanders, R. et al. Evaluation of digital PCR for absolute DNA quantification. Anal. Chem. 83, 6474-6484 (2011).
6. Lo, Y. M. et al. Digital PCR for the molecular detection of fetal chromosomal aneuploidy. Proc. Natl. Acad. Sci. USA 104, 13116-13121 (2007).
7. White, R., Blainey, P., Fan, H. & Quake, S. Digital PCR provides sensitive and absolute calibration for high throughput sequencing. BMC Genomics 10, 116-121 (2009).
8. Pohl', G. & Shih, I. M. Principle and applications of digital PCR. Expert Rev. Mol. Diagn. 4, 41-47 (2004).
9. Pinheiro, L. B. et al. Evaluation of a Droplet Digital Polymerase Chain Reaction Format for DNA Copy Number Quantification. Anal. Chem. 84, 1003-1011 (2012).
10. Kalinina, O., Lebedeva, I., Brown, J. & Silver, J. Nanoliter scale PCR with TaqMan detection. Nucleic Acids Res. 25, 1999-2004 (1997).
11. Zhong, Q. et al. Multiplex digital PCR: breaking the one target per color barrier of quantitative PCR. Lab on a Chip 11, 2167-2174 (2011).
12. Dong, L., Meng, Y., Wang, J. & Liu, Y. Evaluation of droplet digital PCR for characterizing plasmid reference material used for quantifying ammonia oxidizer and denitrifier. Anal. Bioanal. Chem. 406, 1701-1712 (2014).
13. Lee, S., Kim, J., Park, K. & Kim, Y. Qualitative PCR method for detection of genetically modified maize lines NK603 and TC1507. Agriculture Chem. Biotech. 47, 185-188 (2004).
14. Milbury, C. et al. Determining lower limits of detection of digital PCR assays for cancer-related gene mutations. Biomolecular Detection and Quantification 1, 8-22 (2014).
15. Huggett, J. F. et al. The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments. Clinical Chem. 59, 892-902 (2013).
16. Weaver, S. et al. Taking qPCR to a higher level: Analysis of CNV reveals the power of high throughput qPCR to enhance quantitative resolution. Methods 50, 271-276 (2010).
17. Dube, S., Qin, J. & Ramakrishnan, R. Mathematical analysis of copy number variation in a DNA sample using digital PCR on a nanofluidic device. PLoS ONE 3, 2876-2882 (2008).
18. BIPM. Joint Committee for guides in metrology working group 2: International vocabulary of metrology-basic and general concepts and associated terms (VIM). (sevres Cedex, 2008).