Taking qPCR to a higher level: Analysis of CNV reveals the power of high throughput qPCR to enhance quantitative resolution

Methods

Volumn 50, Issue 4, 2010, Pages 271-276

  Weaver, Suzanne ^a   Dube, Simant ^a   Mir, Alain ^a   Qin, Jian ^a   Sun, Gang ^a   Ramakrishnan, Ramesh ^a   Jones, Robert C ^a   Livak, Kenneth J ^a  

^a FLUIDIGM CORPORATION   (United States)

Author keywords

CNV; Copy number variation; Digital PCR; High throughput qPCR; Microfluidic array; qPCR; Real time PCR

Indexed keywords

ACCURACY; ARTICLE; DATA ANALYSIS; ERROR; EXPERIMENT; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; METHODOLOGY; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; X CHROMOSOME;

CHROMOSOMES, HUMAN, X; DNA COPY NUMBER VARIATIONS; GENE DOSAGE; HUMANS; RECEPTORS, G-PROTEIN-COUPLED; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 77649235168     PISSN: 10462023     EISSN: 10959130     Source Type: Journal    
DOI: 10.1016/j.ymeth.2010.01.003     Document Type: Article

References (25)

1. Zimmermann B., Holzgreve W., Wenzel F., and Hahn S. Clin. Chem. 48 (2002) 362-363
2. Bubner B., Gase K., and Baldwin I.T. BMC Biotechnol. 4 (2004) 14-23
3. Shi L., et al. Nat. Biotechnol. 24 (2006) 1151-1161
4. Canales R.D., et al. Nat. Biotechnol. 24 (2006) 1115-1122
5. Pinkel D., Segraves R., Sudar D., Clark S., Poole J., Kowbel D., Collins C., Kuo W.-L., Chen C., Zhai Y., Dairkee S.H., Ljung B., Gray J.W., and Albertson D.G. Nat. Genet. 20 (1998) 207-211
6. Hosono N., Kubo M., Tsuchiya Y., Sato H., Kitamoto T., Saito S., Ohnishi Y., and Nakamura Y. Human Mutat. 29 (2008) 182-189
7. Morrison T., Hurley J., Garcia J., Yoder K., Katz A., Roberts D., Cho J., Kanigan T., Ilyin S.E., Horowitz D., Dixon J.M., and Brenan C.J.H. Nucleic Acids Res. 34 (2006) e123-e131
8. Dahl A., Sultan M., Jung A., Schwartz R., Lange M., Steinwand M., Livak K.J., Lehrach H., and Nyarsik L. Biomed. Microdevices 9 (2007) 307-314
9. Spurgeon S.L., Jones R.C., and Ramakrishnan R. PLoS ONE 3 (2008) e1662-e1668
10. Higuchi R., Fockler C., Dollinger G., and Watson R. Nat. Biotechnol. 11 (1993) 1026-1030
11. Simmonds P., Balfe P., Peuherer J.F., Ludlam C.A., Bishop J.O., and Leigh Brown A.J. J. Virol. 64 (1990) 865-872
12. Sykes P.J., Neoh S.H., Brisco M.J., Hughes E., Condon J., and Morley A.A. BioTechniques 13 (1992) 444-449
13. Vogelstein B., and Kinzler K.W. Proc. Natl. Acad. Sci. USA 96 (1999) 9236-9241
14. Jarvius J., Melin J., Göransson J., Stenberg J., Fredriksson S., Gonzalez-Rey C., Bertilsson S., and Nilsson M. Nat. Methods 3 (2006) 725-727
15. Lo Y.M.D., Lun F.M.F., Chan K.C.A., Tsui N.B.Y., Chong K.C., Lau T.K., Leung T.Y., Zee B.C.Y., Cantor C.R., and Chiu R.W.K. Proc. Natl. Acad. Sci. USA 104 (2007) 13116-13121
16. Qin J., Jones R.C., and Ramakrishnan R. Nucleic Acids Res. 36 (2008) e116-e123
17. Dube S., Qin J., and Ramakrishnan R. PLoS ONE 3 (2008) e2876-e2883
18. Livak K.J., and Schmittgen T.D. Methods 25 (2001) 402-408
19. Schäffeler E., Schwab M., Eichelbaum M., and Zanger U.M. Human Mutat. 22 (2003) 476-485
20. Wu Y.L., Savelli S.L., Yang Y., Zhou B., Rovin B.H., Birmingham D.J., Nagaraja H.N., Hebert L.A., and Yu C.Y. J. Immunol. 179 (2007) 3012-3025
21. Lee J.H., and Jeon J.T. Cytogenet. Genome Res. 123 (2008) 333-342
22. Taylor J.R. An Introduction to Error Analysis (1982), University Science Books, New York
23. Kubista M., Eliasson J., Lennerås M., Andersson S., and Sjöback R. Eppendorf BioNews 29 (2008) 7-8
24. Sindelka R., Jonák J., Hands R., Bustin S.A., and Kubista M. Nucleic Acids Res. 36 (2008) 387-392
25. Tichopad A., Kitchen R., Riedmaier I., Becker C., Ståhlberg A., and Kubista M. Clin. Chem. 55 (2009) 1816-1823