Wx gene in diploid wheat: molecular characterization of five novel alleles from einkorn (Triticum monococcum L. ssp. monococcum) and T. urartu

Molecular Breeding

Volumn 34, Issue 3, 2014, Pages 1137-1146

  Ortega, Raúl ^a   Guzmán, Carlos ^a   Alvarez, Juan B ^a  

^a UNIVERSITY OF CÓRDOBA   (Spain)

Author keywords

Einkorn; Molecular characterization; Starch; T. urartu; Wx gene

Indexed keywords

BIOSYNTHESIS; DNA SEQUENCES; FOOD PRODUCTS; GENES; PROTEINS;

BREAD WHEAT; EINKORN; GENES ENCODE; GRANULE-BOUND STARCH SYNTHASE; MOLECULAR CHARACTERIZATION; T URARTU; TRITICUM; WAXY PROTEINS; WHEAT SEEDS; WX GENE;

STARCH;

EID: 84939879694     PISSN: 13803743     EISSN: 15729788     Source Type: Journal    
DOI: 10.1007/s11032-014-0105-4     Document Type: Article

References (49)

1. Ainsworth C, Clark J, Balsdon J (1993) Expression, organisation and structure of the genes encoding the waxy protein (granule-bound starch synthase) in wheat. Plant Mol Biol 22:67–82
2. Baldwin PM (2001) Starch granule-associated protein and polypeptides: a review. Starch/Stärcke 53:475–503
3. Brandolini A, Vaccino P, Boggini G, Özkan H, Kilian B, Salamini F (2006) Quantification of genetic relationships among A genomes of wheats. Genome 49:297–305
4. Caballero L, Bancel E, Debiton C, Branlard G (2008) Granule-bound starch synthase (GBSS) diversity of ancient wheat and related species. Plant Breed 127:548–553
5. Chao S, Sharp PJ, Worland AJ, Warham EJ, Koebner RMD, Gale MD (1989) RFLP-based genetic maps of wheat homoeologous group 7 chromosomes. Theor Appl Genet 78:495–504
6. Dvorak J, McGuire PE, Cassidy B (1988) Apparent source of the A genomes of wheats inferred from polymorphism in abundance and restriction fragment length of repeated nucleotide sequences. Genome 30:680–689
7. Dvorak J, Pd Terlizzi, Zhang H-B, Resta P (1993) The evolution of polyploid wheats: identification of the A genome donor species. Genome 36:21–31
8. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791
9. Feuillet C, Langridge P, Waugh R (2008) Cereal breeding takes a walk on the wild side. Trends Genet 24:24–32
10. u1a) from Triticum urartu Thum. ex Gandil. Genet Resour Crop Evol 59:971–979
11. Guzmán C, Caballero L, Alvarez JB (2009) Variation in Spanish cultivated einkorn wheat (Triticum monococcum L. ssp. monococcum) as determined by morphological traits and waxy proteins. Genet Resour Crop Evol 56:601–604
12. Guzmán C, Caballero L, Moral A, Alvarez JB (2010) Genetic variation for waxy proteins and amylose content in Spanish spelt wheat (Triticum spelta L.). Genet Resour Crop Evol 57:721–725
13. Guzmán C, Caballero L, Alvarez JB (2011) Molecular characterisation of the Wx-B1 allelic variants identified in cultivated emmer wheat and comparison with those of durum wheat. Mol Breed 28:403–411
14. Guzmán C, Caballero L, Martín LM, Alvarez JB (2012) Waxy genes from spelt wheat: new alleles for modern wheat breeding and new phylogenetic inferences about the origin of this species. Ann Bot 110:1161–1171
15. Huang XQ, Brûlé-Babel A (2012) Sequence diversity, haplotype analysis, association mapping and functional marker development in the waxy and starch synthase IIa genes for grain-yield-related traits in hexaploid wheat (Triticum aestivum L.). Mol Breed 30:627–635
16. Kiribuchi-Otobe C, Nagamine T, Yanagisawa T, Ohnishi M, Yamaguchi I (1997) Production of hexaploid wheats with waxy endosperm character. Cereal Chem 74:72–74
17. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
18. Leterrier M, Holappa LD, Broglie KE, Beckles DM (2008) Cloning, characterisation and comparative analysis of a starch synthase IV gene in wheat: functional and evolutionary implications. BMC Plant Biol 8:98
19. Li W, Gao Z, Xiao W, Wei YM, Liu YX, Chen GY, Pu ZE, Chen HP, Zheng YL (2012) Molecular diversity of restriction enzyme sites, Indels and upstream open reading frames (uORFs) of 5′ untranslated regions (UTRs) of Waxy genes in Triticum L. and Aegilops L. species. Genet Resour Crop Evol 59:1625–1647
20. Librado P, Rozas J (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25:1451–1452
21. Liu YX, Li W, Wei YM, Chen GY, Zheng YL (2009) Molecular characterization of the waxy gene in einkorn wheat. J Plant Sci 4:114–121
22. Mason-Gamer RJ, Weil CF, Kellogg EA (1998) Granule-bound starch synthase: structure, function, and phylogenetic utility. Mol Biol Evol 15:1658–1673
23. McIntosh RA, Yamazaki Y, Dubcovsky J, Rogers WJ, Morris C, Appels R, Xia XC (2013) Catalogue of gene symbols for wheat. http://www.shigen.nig.ac.jp/wheat/komugi/genes/macgene/2013/GeneSymbol.pdf. Accessed 22 Apr 2014
24. Miller TE, Reader SM (1980) Variation in the meiotic chromosome pairing of hybrids between hexaploid and diploid wheats. Cereal Res Commun 8:477–483
25. Miura H, Sugawara A (1996) Dosage effects of the three Wx genes on amylose synthesis in wheat endosperm. Theor Appl Genet 93:1066–1070
26. Monari AM, Simeone MC, Urbano M, Margiotta B, Lafiandra D (2005) Molecular characterization of new waxy mutants identified in bread and durum wheat. Theor Appl Genet 110:1481–1489
27. Murai J, Taira T, Ohta D (1999) Isolation and characterization of the three Waxy genes encoding the granule-bound starch synthase in hexaploid wheat. Gene 234:71–79
28. Nakamura T, Yamamori M, Hirano H, Hidaka S, Nagamine T (1995) Production of waxy (amylose-free) wheats. Mol Gen Genet 248:253–259
29. Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New York
30. Ortega R, Alvarez JB, Guzmán C (2014) Characterization of the Wx gene in diploid Aegilops species and its potential use in wheat breeding. Genet Resour Crop Evol 61:369–382
31. Rawat N, Sehgal SK, Joshi A, Rothe N, Wilson DL, McGraw N, Vadlani PV, Li W, Gill BS (2012) A diploid wheat TILLING resource for wheat functional genomics. BMC Plant Biol 12:205
32. Rice P, Longden I, Bleasby A (2000) EMBOSS: the European molecular biology open software suite. Trends Genet 16:276–277
33. Rodriguez-Quijano M, Vázquez JF, Carrillo JM (2004) Waxy proteins and amylose content in diploid Triticeae species with genomes A, S and D. Plant Breed 123:294–296
34. Sim NL, Kumar P, Hu J, Henikoff S, Schneider G, Ng PC (2012) SIFT web server: predicting effects of amino acid substitutions on proteins. Nucleic Acids Res 40(W1):W452–W457
35. Slade AJ, Fuerstenberg SI, Loeffler D, Steine MN, Facciotti D (2005) A reverse genetic, nontransgenic approach to wheat crop improvement by TILLING. Nat Biotechnol 23:75–81
36. Stacey J, Isaac P (1994) Isolation of DNA from plants. In: Isaac PG (ed) Methods in molecular biology: protocols for nucleic acid analysis by non-radiactive probes. Humana Press, Totawa, pp 9–15
37. Tamura K, Nei M, Kumar S (2004) Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci USA 101:11030–11035
38. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739
39. Urbano M, Margiotta B, Colaprico G, Lafiandra D (2002) Waxy proteins in diploid, tetraploid and hexaploid wheats. Plant Breed 121:465–469
40. Watterson GA (1975) On the number of segregating sites in genetical models without recombination. Theor Popul Biol 7:256–276
41. Wu Y, Weber JL, Vladutiu GD, Tarnopolsky MA (2011) Six novel mutations in the myophosphorylase gene in patients with McArdle disease and a family with pseudo-dominant inheritance pattern. Mol Genet Metab 104:587–591
42. Yamamori M (2009) Amylose content and starch properties generated by five variant Wx alleles for granule-bound starch synthase in common wheat (Triticum aestivum L.). Euphytica 165:607–614
43. Yamamori M, Guzmán C (2013) SNPs and an insertion sequence in five Wx-A1 alleles as factors for variant Wx-A1 protein in wheat. Euphytica 192:325–338
44. Yamamori M, Yamamoto K (2011) Effects of two novel Wx-A1 alleles of common wheat (Triticum aestivum L.) on amylose and starch properties. J Cereal Sci 54:229–235
45. Yamamori M, Nakamura T, Endo TR, Nagamine T (1994) Waxy protein deficiency and chromosomal location of coding genes in common wheat. Theor Appl Genet 89:179–184
46. Yamamori M, Fujita S, Hayakawa K, Matsuki J, Yasui T (2000) Genetic elimination of a starch granule protein, SGP-1, of wheat generates an altered starch with apparent high amylose. Theor Appl Genet 101:21–29
47. Yan L, Bhave M (2001) Characterization of waxy proteins and waxy genes of Triticum timopheevii and T. zhukovskyi and implications for evolution of wheat. Genome 44:582–588
48. Yan L, Bhave M, Fairclough R, Konik C, Rahman S, Appels R (2000) The genes encoding granule-bound starch synthases at the waxy loci of the A, B, and D progenitors of common wheat. Genome 43:264–272
49. Yasui T, Sasaki T, Matsuki J, Yamamori M (1997) Waxy endosperm mutants of bread wheat (Triticum aestivum L.) and their starch properties. Breed Sci 47:161–163