Glutamate: Where does it come from and where does it go?

Neurochemistry International

Volumn 88, Issue , 2015, Pages 47-52

  Olsen, Grethe M ^a   Sonnewald, Ursula ^a  

^a NORWEGIAN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Norway)

Author keywords

Anaplerosis; Astrocytes; Cataplerosis; Glutamate; Lactate; Neurons

Indexed keywords

ASPARTIC ACID; CARBON 13; GLUTAMIC ACID; MALIC ACID; PYRUVIC ACID; TRICARBOXYLIC ACID; GLUTAMINE;

AMINO ACID METABOLISM; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; ASTROCYTE; BRAIN METABOLISM; CATAPLEROSIS; CITRIC ACID CYCLE; CONCENTRATION (PARAMETERS); FEMALE; HOMEOSTASIS; INCUBATION TIME; MASS FRAGMENTOGRAPHY; MASS SPECTROMETRY; METABOLISM; MOUSE; NERVE CELL; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; ANIMAL; BRAIN; CELL CULTURE; DOSE RESPONSE; DRUG EFFECTS; PREGNANCY;

ANIMALS; ASTROCYTES; BRAIN; CELLS, CULTURED; DOSE-RESPONSE RELATIONSHIP, DRUG; FEMALE; GLUTAMIC ACID; GLUTAMINE; MICE; NEURONS; PREGNANCY;

EID: 84939571473     PISSN: 01970186     EISSN: 18729754     Source Type: Journal    
DOI: 10.1016/j.neuint.2014.11.006     Document Type: Article

References (39)

1. P.M. Alves, R. Nunes, Zhang C., C.D. Maycock, U. Sonnewald, M.J. Carrondo, and et al. Metabolism of [3-13C]malate in primary cultures of mouse astrocytes Dev. Neurosci 22 2000 456 462
2. A.I. Amaral, A.P. Teixeira, U. Sonnewald, and P.M. Alves Estimation of intracellular fluxes in cerebellar neurons after hypoglycemia: importance of the pyruvate recycling pathway and glutamine oxidation J. Neurosci. Res 89 2011 700 710
3. L.K. Bak, H.S. Waagepetersen, T.M. Melo, A. Schousboe, and U. Sonnewald Complex glutamate labeling from [U-13C]glucose or [U-13C]lactate in co-cultures of cerebellar neurons and astrocytes Neurochem. Res 32 2007 671 680
4. I.J. Bakken, S.F. Johnsen, L.R. White, G. Unsgard, J. Aasly, and U. Sonnewald NMR spectroscopy study of the effect of 3-nitropropionic acid on glutamate metabolism in cultured astrocytes J. Neurosci. Res 47 1997 642 649
5. I.J. Bakken, L.R. White, J. Aasly, G. Unsgard, and U. Sonnewald Lactate formation from [U-13C]aspartate in cultured astrocytes: compartmentation of pyruvate metabolism Neurosci. Lett 237 1997 117 120
6. I.J. Bakken, L.R. White, J. Aasly, G. Unsgard, and U. Sonnewald [U-13C]aspartate metabolism in cultured cortical astrocytes and cerebellar granule neurons studied by NMR spectroscopy Glia 23 1998 271 277
7. I.J. Bakken, L.R. White, G. Unsgard, J. Aasly, and U. Sonnewald [U-13C]glutamate metabolism in astrocytes during hypoglycemia and hypoxia J. Neurosci. Res 51 5 1998 636 645
8. K. Biemann The mass spectra of isotopically labeled molecules Mass Spectrometry; Organic Chemical Applications 1962 McGraw-Hill New York 223 227
9. S. Cerdan, B. Kunnecke, and J. Seelig Cerebral metabolism of [1,2-13C2]acetate as detected by in vivo and in vitro 13C NMR J. Biol. Chem 265 1990 12916 12926
10. F. Cruz, S.R. Scott, I. Barroso, P. Santisteban, and S. Cerdan Ontogeny and cellular localization of the pyruvate recycling system in rat brain J. Neurochem 70 1998 2613 2619
11. F. Cruz, M. Villalba, M.A. Garcia-Espinosa, P. Ballesteros, E. Bogonez, J. Satrustegui, and et al. Intracellular compartmentation of pyruvate in primary cultures of cortical neurons as detected by 13C NMR spectroscopy with multiple 13C labels J. Neurosci. Res 66 2001 771 781
12. G.A. Dienel Brain lactate metabolism: the discoveries and the controversies JCBFM 32 7 2012 1107 1138
13. J.M.1. Duarte, B. Lanz, and R. Gruetter Compartmentalized cerebral metabolism of [1,6-13C]glucose determined by in vivo 13C NMR spectroscopy at 14.1 T Front. Neuroenerget 3 2011 3 10.3389/fnene.2011.00003
14. M.S. Goyal, M. Hawrylycz, J.A. Miller, A.Z. Snyder, and M.E. Raichle Aerobic glycolysis in the human brain is associated with development and neotenous gene expression Cell Metab 19 2014 49 57
15. A. Haberg, Qu H., I.J. Bakken, L.M. Sande, L.R. White, O. Haraldseth, and et al. In vitro and ex vivo 13C-NMR spectroscopy studies of pyruvate recycling in brain Dev. Neurosci 20 1998 389 398
16. B. Hassel, and U. Sonnewald Glial formation of pyruvate and lactate from TCA cycle intermediates: implications for the inactivation of transmitter amino acids? J. Neurochem 65 1995 2227 2234
17. A. Lapidot, and A. Gopher Cerebral metabolic compartmentation. Estimation of glucose flux via pyruvate carboxylase/pyruvate dehydrogenase by 13C NMR isotopomer analysis of D-[U-13C]glucose metabolites J. Biol. Chem 269 1994 27198 27208
18. T.P. Mawhinney, R.S. Robinett, A. Atalay, and M.A. Madson Analysis of amino acids as their tert.-butyldimethylsilyl derivatives by gas-liquid chromatography and mass spectrometry J. Chromatogr 358 1986 231 242
19. M.C. McKenna, G.A. Dienel, U. Sonnewald, H.S. Waagepetersen, and A. Schousboe Energy metabolism of the brain S.T. Brady, G.J. Siegel, R.W. Albers, D.L. Price, Basic Neurochemistry: Principles of Molecular, Cellular, and Medical Neurobiology eight ed. 2012 Elsevier Academic Press Oxford, UK 200 234
20. M.C. McKenna, J.T. Tildon, J.H. Stevenson, Huang X., and K.G. Kingwell Regulation of mitochondrial and cytosolic malic enzymes from cultured rat brain astrocytes Neurochem. Res 20 1995 1491 1501
21. M.C. McKenna, U. Sonnewald, Huang X., J. Stevenson, and H.R. Zielke Exogenous glutamate concentration regulates the metabolic fate of glutamate in astrocytes J. Neurochem 66 1996 386 393
22. M.C. McKenna, J.H. Stevenson, Huang X., J.T. Tildon, C.L. Zielke, and I.B. Hopkins Mitochondrial malic enzyme activity is much higher in mitochondria from cortical synaptic terminals compared with mitochondria from primary cultures of cortical neurons or cerebellar granule cells Neurochem. Int 36 2000 451 459
23. M.C. McKenna, H.S. Waagepetersen, A. Schousboe, and U. Sonnewald Neuronal and astrocytic shuttle mechanisms for cytosolic-mitochondrial transfer of reducing equivalents: current evidence and pharmacological tools Biochem. Pharmacol 71 2006 399 407
24. T.M. Melo, A. Nehlig, and U. Sonnewald Neuronal-glial interactions in rats fed a ketogenic diet Neurochem. Int 48 2006 498 507
25. T.S. Morken, E. Brekke, A. Haberg, M. Wideroe, A.M. Brubakk, and U. Sonnewald Altered astrocyte-neuronal interactions after hypoxia-ischemia in the neonatal brain in female and male rats Stroke 45 9 2014 2777 2785
26. E. Olstad, G.M. Olsen, Qu H., and U. Sonnewald Pyruvate recycling in cultured neurons from cerebellum J. Neurosci. Res 85 2007 3318 3325
27. E. Olstad, Qu H., and U. Sonnewald Long-term kainic acid exposure reveals compartmentation of glutamate and glutamine metabolism in cultured cerebellar neurons Neurochem. Int 50 2007 1004 1013
28. M.S. Patel The relative significance of CO2-fixing enzymes in the metabolism of rat brain J. Neurochem 22 1974 717 724
29. A. Schousboe, E. Meier, J. Drejer, and L. Hertz Preparation of primary cultures of mouse (rat) cerebellar granule cells A. Shahar, A Dissection and Tissue Culture Manual for the Nervous System 1989 Alan R. Liss New York 183 186
30. A. Schousboe, L.K. Bak, H.M. Sickmann, U. Sonnewald, and H.S. Waagepetersen Energy substrates to support glutamatergic and GABAergic synaptic function: role of glycogen, glucose and lactate Neurotox. Res 12 2007 263 268
31. R.P. Shank, G.S. Bennett, S.O. Freytag, and G.L. Campbell Pyruvate carboxylase: an astrocyte-specific enzyme implicated in the replenishment of amino acid neurotransmitter pools Brain Res 329 1985 364 367
32. U. Sonnewald Glutamate synthesis has to be matched by its degradation - where do all the carbons go? J. Neurochem 2014 10.1111/jnc.12812 Epub ahead of print
33. U. Sonnewald, and C. Rae Pyruvate carboxylation in different model systems studied by 13C MRS Neurochem. Res 35 12 2010 1916 1921
34. U. Sonnewald, N. Westergaard, S.B. Petersen, G. Unsgard, and A. Schousboe Metabolism of [U-13C]glutamate in astrocytes studied by 13C NMR spectroscopy: incorporation of more label into lactate than into glutamine demonstrates the importance of the tricarboxylic acid cycle J. Neurochem 61 1993 1179 1182
35. U. Sonnewald, N. Westergaard, P. Jones, A. Taylor, H.S. Bachelard, and A. Schousboe Metabolism of [U-13C5] glutamine in cultured astrocytes studied by NMR spectroscopy: first evidence of astrocytic pyruvate recycling J. Neurochem 67 1996 2566 2572
36. U. Sonnewald, E. Olstad, Qu H., Z. Babot, R. Cristofol, C. Sunol, and et al. First direct demonstration of extensive GABA synthesis in mouse cerebellar neuronal cultures J. Neurochem 91 2004 796 803
37. U. Sonnewald, A. Schousboe, Qu H., and H.S. Waagepetersen Intracellular metabolic compartmentation assessed by 13C magnetic resonance spectroscopy Neurochem. Int 45 2004 305 310
38. H.S. Waagepetersen, Qu H., L. Hertz, U. Sonnewald, and A. Schousboe Demonstration of pyruvate recycling in primary cultures of neocortical astrocytes but not in neurons Neurochem. Res 27 2002 1431 1437
39. Yu A.C., J. Drejer, L. Hertz, and A. Schousboe Pyruvate carboxylase activity in primary cultures of astrocytes and neurons J. Neurochem 41 1983 1484 1487