[U-13C]glutamate metabolism in astrocytes during hypoglycemia and hypoxia

Journal of Neuroscience Research

Volumn 51, Issue 5, 1998, Pages 636-645

  Bakken, Inger Johanne ^a   White, Linda R ^a   Unsgård, Geirmund ^a   Aasly, Jan ^a   Sonnewald, Ursula ^a  

^a NORWEGIAN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Norway)

Author keywords

13C NMR spectroscopy; U 13C Glutamate; Energy metabolism

Indexed keywords

ASPARTIC ACID; CARBON 13; GLUCOSE; GLUTAMIC ACID; GLUTAMINE; IODOACETIC ACID; LACTIC ACID; OXYGEN; PYROGLUTAMIC ACID; 4 KETOPROLINE; 4-KETOPROLINE; CARBON; DRUG DERIVATIVE; PROLINE;

AMINO ACID METABOLISM; ANIMAL CELL; ARTICLE; ASTROCYTE; CARBON NUCLEAR MAGNETIC RESONANCE; CELL HYPOXIA; CITRIC ACID CYCLE; CONTROLLED STUDY; GLYCOLYSIS; HYPOGLYCEMIA; MOUSE; NERVE CELL CULTURE; NEWBORN; NONHUMAN; PRIORITY JOURNAL; ANIMAL; CELL CULTURE; CYTOLOGY; METABOLISM; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PHYSIOLOGY; PREFRONTAL CORTEX;

ANIMALS; ASPARTIC ACID; ASTROCYTES; CARBON ISOTOPES; CELL HYPOXIA; CELLS, CULTURED; GLUCOSE; GLUTAMIC ACID; GLYCOLYSIS; HYPOGLYCEMIA; IODOACETATES; IODOACETIC ACID; LACTIC ACID; MAGNETIC RESONANCE SPECTROSCOPY; MICE; PREFRONTAL CORTEX; PROLINE;

EID: 0032008541     PISSN: 03604012     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-4547(19980301)51:5<636::AID-JNR11>3.0.CO;2-0     Document Type: Article

References (44)

1. Bakken IJ, Johnsen SF, White LR, Unsgård G, Aasly J, Sonnewald U (1997): NMR spectroscopy study of the effect of 3-nitropropionic acid on glutamate metabolism in cultured astrocytes. J Neurosci Res 47:642-649.
2. Beal MF, Hyman BT, Koroshetz W (1993): Do defects in mitochondrial energy metabolism underlie the pathology of neurodegenerative diseases? Trends Neurosci 16:125-131.
3. Benveniste H, Drejer J, Schousboe A, Diemer NH (1984): Elevation of extracellular concentrations of glutamate and aspartate in rat hippocampus during transient cerebral ischemia monitored by intracerebral microdialysis. J Neurochem 43:1369-1374.
4. Butcher SP, Sandberg M, Hagberg H, Hamberger A (1987): Cellular origins of endogenous amino acids released into extracellular fluid of the rat striatum during severe insulin-induced hypoglycemia. J Neurochem 48:722-728.
5. Cammer W (1990): Glutamine synthetase in the central nervous system is not confined to astrocytes. J Neuroimmunol 26:173-178.
6. Cataldo AM, Broadwell RD (1986): Cytochemical identification of cerebral glycogen and glucose-6-phosphatase activity under normal and experimental conditions: neurons and glia. J Electron Microsc Tech 3:413-437.
7. 13C NMR. J Biol Chem 265:12916-12926.
8. Chaudhry FA, Lehre KP, van Lookeren Campagne M, Ottersen OP, Danbolt NC, Storm Mathisen J (1995): Glutamate transporters in glial plasma membranes: highly differentiated localizations revealed by quantitative ultrastructural immunocytochemistry. Neuron 15:711-720.
9. Dringen R, Hamprecht B (1993): Differences in glycogen metabolism in astroglia-rich primary cultures and sorbitol-selected astroglial cultures derived from mouse brain. Glia 8:143-149.
10. Erecinska M (1987): The neurotransmitter amino acid transport systems: a fresh outlook on an old problem. Biochem Pharmacol 36:3547-3555.
11. Erecinska M, Nelson D, Wilson DF, Silver IA (1984): Neurotransmitter amino acids in the CNS. I. Regional changes in amino acid levels in rat brain during ischemia and reperfusion. Brain Res 304:9-22.
12. 15N]glutamate. J Neurochem 51:892-902.
13. Gegelashvili G, Civenni G, Racagni G, Danbolt NC, Diemer NH, Schousboe A (1996): Glutamate receptor agonists up-regulate glutamate transporter GLAST in astrocytes. NeuroReport 8:261-265.
14. Gemba T, Oshima T, Ninomiya M (1994): Glutamate efflux via the reversal of the sodium-dependent glutamate transporter caused by glycolytic inhibition in rat brain astrocytes. Neuroscience 33:789-795.
15. Globus MY-T, Busto R, Dietrich WD, Martinez E, Valdes I, Ginsberg MD (1988): Effects of ischemia on the in vivo release of strialal dopamine, glutamate, and γ-aminobutyric acid studied by intracerebral microdialysis. J Neurochem 51:1464.
16. Hertz L, Juurlink BHJ, Hertz E, Fosmark H, Schousboe A (1989): Preparation of primary cultures of mouse (rat) astrocytes. In Shahar A, De Vellis J, Vernadakis A, Haber B (eds): "A Dissection and Tissue Culture Manual tor The Nervous System." New York: Alan R. Liss, Inc., pp 105-108.
17. Huang R, Shuaib A, Hertz L (1993): Glutamate uptake and glutamate content in primary cultures of mouse astrocytes during anoxia, substrate deprivation and simulated ischemia under normothermic and hypothermic conditions. Brain Res 618:346-351.
18. Kanai Y, Hediger MA (1992): Primary structure and functional characterization of a high-affinity glutamate transporter. Nature 360:467-471.
19. Kauppinen RA, Enkvist K, Holopainen I, Akerman KE (1988): Glucose deprivation depolarizes plasma membrane of cultured astrocytes and collapses transmembrane potassium and glutamate gradients. Neuroscience 26:283-289.
20. Koizumi J (1974): Glycogen in the central nervous system. Prog Histochem Cytochem 6:1-35.
21. Longuemare MC, Swanson RA (1995): Excitatory amino acid release from astrocytes during energy failure by reversal of sodium-dependent uptake. J Neurosci Res 40:379-386.
22. Makar TK, Nedergaard M, Preuss A, Hertz L, and Cooper AJL (1994): Glutamine transaminase K and ω-amidase activities in primary cultures of astrocytes and neurons and in embryonic chick forebrain: marked induction of brain glutamine K at time of hatching. J Neurochem 62:1983-1988.
23. McKenna MC, Sonnewald U, Huang X, Stevenson J, Zielke RH (1996): Exogenous glutamate concentration regulates the metabolic fate of glutamate in astrocytes. J Neurochem 66:386-393.
24. Nicholls D, Attwell D (1990): The release and uptake of excitatory amino acids. Trends Pharmacol Sci 11:462-468.
25. Norenberg MD, Martinez Hernandez A (1979): Fine structural localization of glutamine synthetase in astrocytes of rat brain. Brain Res 161:303-310.
26. O'Neill CM, Ball SG, Vaughan PFT (1994): Effects of ischaemic conditions on uptake of glutamate, aspartate, and noradrenaline by cell lines derived from the human nervous system. J Neurochem 63:603-611.
27. Pauwels PJ, Opperdoes FR, Trouet A (1985): Effects of antimycin. glucose deprivation, and serum on cultures of neurons, astrocytes, and neuroblastoma cells. J Neurochem 44:143-148.
28. Pellerin L, Magistretti PJ (1994): Glutamate uptake into astrocytes stimulates aerobic glycolysis: a mechanism coupling neuronal activity to glucose utilization. Proc Natl Acad Sci USA 91:10625-10629.
29. Pines G, Danbolt NC, Bjoras M, Zhang Y, Bendahan A, Eide L, Koepsell H, Storm Mathisen J, Seeberg E, Kanner BI (1992): Cloning and expression of a rat brain L-glutamate transporter. Nature 360:464-467.
30. Sandberg S, Butcher SP, Hagberg H (1986): Extracellular overflow of neuroactive amino acids during severe insulin-induced hypoglycemia: In vivo dialysis of the rat hippocampus. J Neurochem 47:178-184.
31. Schousboe A, Westergaard N, Sonnewald U, Petersen SB, Huang R, Peng L, Hertz L (1993): Glutamate and glutamine metabolism and compartmentation in astrocytes. Dev Neurosci 15:359-366.
32. Shank RP, Bennet GS, Freytag SO, Campbell GL (1985): Pyruvate carboxylase: an astrocyte-specific enzyme implicated in the replenishment of amino acid neurotransmitter pools. Brain Res 329:364-367.
33. 13C NMR spectroscopy: incorporation of more label into lactate than into glutamine demonstrates the importance of the TCA cycle. J Neurochem 61:1179-1182.
34. 13C]glutamine in cultured astrocytes studied by NMR spectroscopy: first evidence of astrocytic pyruvate recycling. J Neurochem 67:2566-2572.
35. Storck T, Schulte S, Hofmann K, Stoffel W (1992): Structure, expression, and functional analysis of a Na(+) -dependent glutamate/aspartate transporter from rat brain. Proc Natl Acad Sci USA 89:10955-10959.
36. Swanson RA (1992): Astrocyte glutamate uptake during chemical hypoxia in vitro. Neurosci Lett 147:143-146.
37. Swanson RA, Choi DW (1993): Glial glycogen stores affect neuronal survival during glucose deprivation in vitro. J Cereb Blood Flow Metab 13:162-169.
38. Swanson RA, Graham SH (1994): Fluorocitrate and fluoroacetate effects on astrocyte metabolism in vitro. Brain Res 664:94-100.
39. Swanson RA, Yu AC, Chan PH, Sharp FR (1990): Glutamate increases glycogen content and reduces glucose utilization in primary astrocyte culture. J Neurochem 54:490-496.
40. Wilker W, Engelmann J, Brand A, Sonnewald U, Leibfritz D (1995): Identification of pyro-glutamate and glucosyl-aminoacids in cell culture media. J Magn Reson Anal 1:20-24.
41. Yu ACH, Hertz L (1983): Metabolic sources of energy in astrocytes. In Hertz L, Kvamme E, McGeer EG, Schousboe A (eds): "Glutamine, Glutamate, and GABA in the Central Nervous System." New York: Alain R. Liss. Inc., pp 431-138.
42. 14C-labeled glutamate in astrocytes in primary cultures. J Neurochem 39:954-960.
43. Yu ACH, Drejer J, Hertz L, Schousboe A (1983): Pyruvate carboxylase activity in primary cultures of astrocytes and neurons. J Neurochem 41:1484-1487.
44. 15N]glutamine: implications for the glutamine-glutamate cycle. J Neurochem 51:843-850.