Quantitative Determination of Luminal and Abluminal Membrane Distributions of Transporters in Porcine Brain Capillaries by Plasma Membrane Fractionation and Quantitative Targeted Proteomics

Journal of Pharmaceutical Sciences

Volumn 104, Issue 9, 2015, Pages 3060-3068

  Kubo, Yoshiyuki ^a   Ohtsuki, Sumio ^b   Uchida, Yasuo ^c   Terasaki, Tetsuya ^c  

^a UNIVERSITY OF TOYAMA   (Japan)

^b KUMAMOTO UNIVERSITY   (Japan)

^c TOHOKU UNIVERSITY   (Japan)

Author keywords

blood brain barrier; localization; mass spectrometry; membrane transport; pharmacoproteomics (PPx); polarity; quantitative targeted absolute proteomics (QTAP); species differences; transporter

Indexed keywords

ABC TRANSPORTER A1; ARGININE; BREAST CANCER RESISTANCE PROTEIN; FATTY ACID TRANSPORTER 1; GLUCOSE TRANSPORTER 1; LYSINE; MEMBRANE PROTEIN; MONOCARBOXYLATE TRANSPORTER 1; MULTIDRUG RESISTANCE PROTEIN 1; MULTIDRUG RESISTANCE PROTEIN 5; ORGANIC ANION TRANSPORTER D; TRANSCRIPTION FACTOR GATA 2; UNCLASSIFIED DRUG; ABC TRANSPORTER; ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); CARRIER PROTEIN; FATTY ACID TRANSPORTER; ORGANIC ANION TRANSPORTER;

ABLUMINAL MEMBRANE; AMINO ACID SEQUENCE; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BLOOD BRAIN BARRIER; BRAIN CAPILLARY; BRAIN CAPILLARY ENDOTHELIAL CELL; CELL FRACTIONATION; CELL MEMBRANE; CELLULAR DISTRIBUTION; DENSITY GRADIENT CENTRIFUGATION; LIMIT OF QUANTITATION; LUMINAL MEMBRANE; NONHUMAN; PORCINE MODEL; PROTEIN CONTENT; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEOMICS; QUANTITATIVE ANALYSIS; SPECIES DIFFERENCE; ULTRACENTRIFUGATION; ANIMAL; BRAIN; CAPILLARY; METABOLISM; PHYSIOLOGY; PIG; PROCEDURES; TRANSPORT AT THE CELLULAR LEVEL;

ANIMALS; ATP BINDING CASSETTE TRANSPORTER 1; ATP-BINDING CASSETTE TRANSPORTERS; BIOLOGICAL TRANSPORT; BLOOD-BRAIN BARRIER; BRAIN; CAPILLARIES; CELL MEMBRANE; FATTY ACID TRANSPORT PROTEINS; GLUCOSE TRANSPORTER TYPE 1; MEMBRANE TRANSPORT PROTEINS; ORGANIC ANION TRANSPORTERS; PROTEOMICS; SODIUM-POTASSIUM-EXCHANGING ATPASE; SWINE;

EID: 84939266400     PISSN: 00223549     EISSN: 15206017     Source Type: Journal    
DOI: 10.1002/jps.24398     Document Type: Article

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