CRISPR/Cas9-based generation of knockdown mice by intronic insertion of artificial microRNA using longer single-stranded DNA

Scientific Reports

Volumn 5, Issue , 2015, Pages

  Miura, Hiromi ^a   Gurumurthy, Channabasavaiah B ^b   Sato, Takehito ^a   Sato, Masahiro ^c   Ohtsuka, Masato ^a,d  

^a TOKAI UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b UNIVERSITY OF NEBRASKA MEDICAL CENTER   (United States)

^c KAGOSHIMA UNIVERSITY   (Japan)

^d TOKAI UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; CAS9 PROTEIN, FRANCISELLA NOVICIDA; ENDONUCLEASE; ENHANCED GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; MICRORNA; OTX2 PROTEIN, MOUSE; SINGLE STRANDED DNA; TRANSCRIPTION FACTOR OTX;

ANIMAL; BIOSYNTHESIS; C57BL MOUSE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; FEMALE; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENE SILENCING; GENETICS; INTRON; MALE; MICROINJECTION; PROCEDURES; REPORTER GENE; RNA INTERFERENCE; TRANSGENIC MOUSE;

ANIMALS; BACTERIAL PROTEINS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; DNA, SINGLE-STRANDED; ENDONUCLEASES; FEMALE; GENE EXPRESSION; GENE KNOCKDOWN TECHNIQUES; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; INTRONS; MALE; MICE, INBRED C57BL; MICE, TRANSGENIC; MICROINJECTIONS; MICRORNAS; MUTAGENESIS, INSERTIONAL; OTX TRANSCRIPTION FACTORS; RNA INTERFERENCE;

EID: 84938922797     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep12799     Document Type: Article

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