Genetic modification in human pluripotent stem cells by homologous recombination and CRISPR/Cas9 system

Methods in Molecular Biology

Volumn 1307, Issue , 2015, Pages 173-190

  Xue, Haipeng ^a   Wu, Jianbo ^a   Li, Shenglan ^a   Rao, Mahendra S ^a   Liu, Ying ^a  

^a NONE

Author keywords

Cas; CRISPR; Gene targeting; Genetic engineering; Genome editing; Homologous recombination; Human induced pluripotent stem cells; Reporter cell line; TALEN; ZFN

Indexed keywords

CRISPR ASSOCIATED PROTEIN; GENOMIC DNA; GUIDE RNA; RESTRICTION ENDONUCLEASE; TRANSCRIPTION ACTIVATOR LIKE EFFECTOR NUCLEASE; TRANSCRIPTION FACTOR SOX1; ZINC FINGER NUCLEASE; TRANSCRIPTION FACTOR SOX;

ANIMAL CELL; ARTICLE; CELL CLONE; CELL ISOLATION; CELL LINEAGE; CELL SURVIVAL; CELLS BY BODY ANATOMY; CONTROLLED STUDY; CRISPR CAS SYSTEM; DNA MODIFICATION; ELECTROPORATION; EMBRYO; FEEDER CELL; GENE CONSTRUCT; GENE TARGETING; GENETIC ENGINEERING; GENETIC TRANSFECTION; HOMOLOGOUS RECOMBINATION; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; HUMAN GENOME; MEMBRANE TRANSPORT; MOLECULAR BIOLOGY; MOUSE; NONHUMAN; PLURIPOTENT STEM CELL; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RNA EDITING; SOUTHERN BLOTTING; STREPTOCOCCUS PYOGENES; ANIMAL; FIBROBLAST; GENE VECTOR; GENETICS; METABOLISM; MUTATION; PROCEDURES; REPORTER GENE;

ANIMALS; BLOTTING, SOUTHERN; CELL SURVIVAL; CRISPR-CAS SYSTEMS; ELECTROPORATION; FIBROBLASTS; GENE TARGETING; GENES, REPORTER; GENETIC ENGINEERING; GENETIC VECTORS; HOMOLOGOUS RECOMBINATION; HUMANS; MICE; MUTATION; PLURIPOTENT STEM CELLS; RNA, GUIDE; SOXB1 TRANSCRIPTION FACTORS; TRANSFECTION;

EID: 84938844899     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/7651_2014_73     Document Type: Article

References (21)

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