Viability of developmental stages of Schistosoma mansoni quantified with xCELLigence worm real-time motility assay (xWORM)

International Journal for Parasitology: Drugs and Drug Resistance

Volumn 5, Issue 3, 2015, Pages 141-148

  Rinaldi, Gabriel ^a   Loukas, Alex ^b   Brindley, Paul J ^a   Irelan, Jeff T ^c   Smout, Michael J ^b  

^a GEORGE WASHINGTON UNIVERSITY SCHOOL OF MEDICINE AND HEALTH SCIENCES   (United States)

^b JAMES COOK UNIVERSITY   (Australia)

^c ACEA Biosciences ^*  (United States)

Author keywords

Helminths; High throughput; Schistosome; Strictly standardized mean difference (SSMD); Viability; XCELLigence

Indexed keywords

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CELL ASSAY; CONTROLLED STUDY; DEVELOPMENTAL STAGE; HATCHING; MOUSE; NONHUMAN; PRIORITY JOURNAL; SCHISTOSOMA; SCHISTOSOMA MANSONI; SCHISTOSOME CERCARIAE; SCHISTOSOMULUM; XCELLIGENCE WORM REAL TIME MOTILITY ASSAY; ANIMAL; BIOASSAY; BIOMPHALARIA; FEMALE; MALE; MOTOR ACTIVITY; OVUM; PHYSIOLOGY;

ANIMALS; BIOLOGICAL ASSAY; BIOMPHALARIA; FEMALE; MALE; MICE; MOTOR ACTIVITY; OVUM; SCHISTOSOMA MANSONI;

EID: 84938693292     PISSN: None     EISSN: 22113207     Source Type: Journal    
DOI: 10.1016/j.ijpddr.2015.07.002     Document Type: Article

References (47)

1. Abdul-Ghani R.A., Loutfy N., Hassan A. Experimentally promising antischistosomal drugs: a review of some drug candidates not reaching the clinical use. Parasitol. Res. 2009, 105(4):899-906.
2. Abdulla M.H., Ruelas D.S., Wolff B., Snedecor J., Lim K.C., Xu F., Renslo A.R., Williams J., Mckerrow J.H., Caffrey C.R. Drug discovery for schistosomiasis: hit and lead compounds identified in a library of known drugs by medium-throughput phenotypic screening. PLoS Negl. Trop. Dis. 2009, 3(7):e478.
3. Albonico M., Allen H., Chitsulo L., Engels D., Gabrielli A.F., Savioli L. Controlling soil-transmitted helminthiasis in pre-school-age children through preventive chemotherapy. PLoS Negl. Trop. Dis. 2008, 2(3):e126.
4. Bardosh K. Global aspirations, local realities: the role of social science research in controlling neglected tropical diseases. Infect. Dis. Poverty 2014, 3(1):35.
5. Birmingham A., Selfors L.M., Forster T., Wrobel D., Kennedy C.J., Shanks E., Santoyo-Lopez J., Dunican D.J., Long A., Kelleher D., Smith Q., Beijersbergen R.L., Ghazal P., Shamu C.E. Statistical methods for analysis of high-throughput RNA interference screens. Nat. Methods 2009, 6(8):569-575.
6. Cioli D., Pica-Mattoccia L., Basso A., Guidi A. Schistosomiasis control: praziquantel forever?. Mol. Biochem. Parasitol. 2014, 195(1):23-29.
7. Cohen J. The Earth is round (p<0.05). Am. Psychol. 1994, 49(12):997-1003.
8. Dalton J.P., Day S.R., Drew A.C., Brindley P.J. A method for the isolation of schistosome eggs and miracidia free of contaminating host tissues. Parasitology 1997, 115(Pt 1):29-32.
9. Evans T., Chapple N. The animal health market. Nat. Rev. Drug Discov. 2002, 1(12):937-938.
10. Falzon L.C., O'neill T.J., Menzies P.I., Peregrine A.S., Jones-Bitton A., Vanleeuwen J., Mederos A. A systematic review and meta-analysis of factors associated with anthelmintic resistance in sheep. Prev. Vet. Med. 2014, 117(2):388-402.
11. Goktug A.N., Ong S.S., Chen T. GUItars: a GUI tool for analysis of high-throughput RNA interference screening data. PLoS One 2012, 7(11):e49386.
12. Hopkins A.L., Witty M.J., Nwaka S. Mission possible. Nature 2007, 449(7159):166-169.
13. Hotez P. Enlarging the "Audacious Goal": elimination of the world's high prevalence neglected tropical diseases. Vaccine 2011, 29(Suppl. 4):D104-D110.
14. Howe S., Zophel D., Subbaraman H., Unger C., Held J., Engleitner T., Hoffmann W.H., Kreidenweiss A. Lactate as a novel quantitative measure of viability in Schistosoma mansoni drug sensitivity assays. Antimicrob. Agents Chemother. 2015, 59(2):1193-1199.
15. James C.E., Hudson A.L., Davey M.W. Drug resistance mechanisms in helminths: is it survival of the fittest?. Trends Parasitol. 2009, 25(7):328-335.
16. Keiser J. In vitro and in vivo trematode models for chemotherapeutic studies. Parasitology 2009, 137(3):589-603.
17. Kirk R.E. Practical significance: a concept whose time has come. Educ. Psychol. Meas. 1996, 56(5):746-759.
18. Kotze A.C., Clifford S., O'grady J., Behnke J.M., Mccarthy J.S. An in vitro larval motility assay to determine anthelmintic sensitivity for human hookworm and Strongyloides species. Am. J. Trop. Med. Hyg. 2004, 71(5):608-616.
19. Kotze A.C., Le Jambre L.F., O'grady J. A modified larval migration assay for detection of resistance to macrocyclic lactones in Haemonchus contortus, and drug screening with Trichostrongylidae parasites. Vet. Parasitol. 2006, 137(3-4):294-305.
20. Kozak K. The Use of Design Specificity in Standardized Mean Difference for Analysis of High Throughput RNA Interference Screens 2012, Springer, Verlag Berlin Heidelberg.
21. Lai Y., Xiang M., Liu S., Li E., Che Y., Liu X. A novel high-throughput nematicidal assay using embryo cells and larvae of Caenorhabditis elegans. Exp. Parasitol. 2014, 139:33-41.
22. Lalli C., Guidi A., Gennari N., Altamura S., Bresciani A., Ruberti G. Development and validation of a luminescence-based, medium-throughput assay for drug screening in Schistosoma mansoni. PLoS Negl. Trop. Dis. 2015, 9(1):e0003484.
23. Mahanty S., Madrid E.M., Nash T.E. Quantitative screening for anticestode drugs based on changes in baseline enzyme secretion by Taenia crassiceps. Antimicrob. Agents Chemother. 2013, 57(2):990-995.
24. Mann V.H., Morales M.E., Rinaldi G., Brindley P.J. Culture for genetic manipulation of developmental stages of Schistosoma mansoni. Parasitology 2010, 137(3):451-462.
25. Mansour N.R., Bickle Q.D. Comparison of microscopy and Alamar blue reduction in a larval based assay for schistosome drug screening. PLoS Negl. Trop. Dis. 2010, 4(8):e795.
26. Marxer M., Ingram K., Keiser J. Development of an in vitro drug screening assay using Schistosoma haematobium schistosomula. Parasites Vectors 2012, 5:165.
27. May R.M. Parasites, people and policy: infectious diseases and the Millennium Development Goals. Trends Ecol. Evol. 2007, 22(10):497-503.
28. Molento M.B. Parasite control in the age of drug resistance and changing agricultural practices. Vet. Parasitol. 2009, 163(3):229-234.
29. Morel C.M. Neglected diseases: under-funded research and inadequate health interventions. Can we change this reality?. EMBO Rep. 2003, 4(Spec. no):S35-S38.
30. Paveley R.A., Mansour N.R., Hallyburton I., Bleicher L.S., Benn A.E., Mikic I., Guidi A., Gilbert I.H., Hopkins A.L., Bickle Q.D. Whole organism high-content screening by label-free, image-based Bayesian classification for parasitic diseases. PLoS Negl. Trop. Dis. 2012, 6(7):e1762.
31. Peak E., Chalmers I.W., Hoffmann K.F. Development and validation of a quantitative, high-throughput, fluorescent-based bioassay to detect schistosoma viability. PLoS Negl. Trop. Dis. 2010, 4(7):e759.
32. Peak E., Hoffmann K.F. Cross-disciplinary approaches for measuring parasitic helminth viability and phenotype. Ann. Acad. Bras. Cienc. 2011, 83(2):649-662.
33. Rinaldi G., Suttiprapa S., Tort J.F., Folley A.E., Skinner D.E., Brindley P.J. An antibiotic selection marker for schistosome transgenesis. Int. J. Parasitol. 2012, 42(1):123-130.
34. Rohde C.B., Zeng F., Gonzalez-Rubio R., Angel M., Yanik M.F. Microfluidic system for on-chip high-throughput whole-animal sorting and screening at subcellular resolution. Proc. Natl. Acad. Sci. U. S. A. 2007, 104(35):13891-13895.
35. Silbereisen A., Tritten L., Keiser J. Exploration of novel in vitro assays to study drugs against Trichuris spp. J. Microbiol. Methods 2011, 87(2):169-175.
36. Smout M.J., Kotze A.C., Mccarthy J.S., Loukas A. A novel high throughput assay for anthelmintic drug screening and resistance diagnosis by real-time monitoring of parasite motility. PLoS Negl. Trop. Dis. 2010, 4(11):e885.
37. Smout M.J., Mulvenna J.P., Jones M.K., Loukas A. Expression, refolding and purification of Ov-GRN-1, a granulin-like growth factor from the carcinogenic liver fluke, that causes proliferation of mammalian host cells. Protein Expr. Purif. 2011, 79(2):263-270.
38. Sommerfeld J., Oduola A.M. Health-related biotechnologies for infectious disease control in Africa: Ethical, Legal and Social Implications (ELSI) of transfer and development. Afr. J. Med. Med. Sci. 2007, 36(Suppl. 1-5).
39. Tong J., Rezai P., Salam S., Selvaganapathy P.R., Gupta B.P. Microfluidic-based electrotaxis for on-demand quantitative analysis of Caenorhabditis elegans' locomotion. J. Vis. Exp. 2013, (75):e50226.
40. Tritten L., Braissant O., Keiser J. Comparison of novel and existing tools for studying drug sensitivity against the hookworm Ancylostoma ceylanicum in vitro. Parasitology 2012, 139(3):348-357.
41. Vistejnova L., Dvorakova J., Hasova M., Muthny T., Velebny V., Soucek K., Kubala L. The comparison of impedance-based method of cell proliferation monitoring with commonly used metabolic-based techniques. Neuro Endocrinol. Lett. 2009, 30(Suppl. 1):121-127.
42. White A.G., Lees B., Kao H.L., Cipriani P.G., Munarriz E., Paaby A.B., Erickson K., Guzman S., Rattanakorn K., Sontag E., Geiger D., Gunsalus K.C., Piano F. DevStaR: high-throughput quantification of C. elegans developmental stages. IEEE Trans. Med. Imaging 2013, 32(10):1791-1803.
43. You H., Mcmanus D.P., Hu W., Smout M.J., Brindley P.J., Gobert G.N. Transcriptional responses of in vivo praziquantel exposure in schistosomes identifies a functional role for calcium signalling pathway member CamKII. PLoS Pathog. 2013, 9(3):e1003254.
44. Zeraik A.E., Galkin V.E., Rinaldi G., Garratt R.C., Smout M.J., Loukas A., Mann V.H., Araujo A.P., Demarco R., Brindley P.J. Reversible paralysis of Schistosoma mansoni by forchlorfenuron, a phenylurea cytokinin that affects septins. Int. J. Parasitol. 2014, 44(8):523-531.
45. Zhang X.D. A new method with flexible and balanced control of false negatives and false positives for hit selection in RNA interference high-throughput screening assays. J. Biomol. Screen. 2007, 12(5):645-655.
46. Zhang X.D. Illustration of SSMD, z score, SSMD*, z*score, and t statistic for hit selection in RNAi high-throughput screens. J. Biomol. Screen. 2011, 16(7):775-785.
47. Zhang X.D., Lacson R., Yang R., Marine S.D., Mccampbell A., Toolan D.M., Hare T.R., Kajdas J., Berger J.P., Holder D.J., Heyse J.F., Ferrer M. The use of SSMD-based false discovery and false nondiscovery rates in genome-scale RNAi screens. J. Biomol. Screen. 2010, 15(9):1123-1131.