YCG063 inhibits Pseudomonas aeruginosa LPS-induced inflammation in human retinal pigment epithelial cells through the TLR2-mediated AKT/NF-κB pathway and ROS-independent pathways

International Journal of Molecular Medicine

Volumn 36, Issue 3, 2015, Pages 808-816

  Hwa Paeng, Sung ^a   Park, Won Sun ^b   Jung, Won Kyo ^c   Lee, Dae Sung ^d   Kim, Gi Young ^e   Choi, Yung Hyun ^f   Seo, Su Kil ^g   Jang, Won Hee ^g   Choi, Jung Sik ^a   Lee, Young Min ^a   Park, Saegwang ^g   Choi, Il Whan ^g  

^a Inje University College of Medicine   (South Korea)

^b Kangwon National University College of Medicine   (South Korea)

^c Pukyong National University   (South Korea)

^d Marine Biodiversity Institute of Korea   (South Korea)

^e Jeju National University   (South Korea)

^f Dong Eui University   (South Korea)

^g Inje University College of Medicine   (South Korea)

Author keywords

Endophthalmitis; Pseudomonas aeruginosa; Reactive oxygen species; Retinal pigment epithelium; Toll like receptor

Indexed keywords

3 (4 METHYLPHENYLSULFONYL) 2 PROPENENITRILE; ANTIINFLAMMATORY AGENT; BACTERIUM LIPOPOLYSACCHARIDE; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INTERCELLULAR ADHESION MOLECULE 1; INTERLEUKIN 6; INTERLEUKIN 8; MONOCYTE CHEMOTACTIC PROTEIN 1; PARTHENOLIDE; PROTEIN KINASE B; REACTIVE OXYGEN METABOLITE; TOLL LIKE RECEPTOR 2; UNCLASSIFIED DRUG; YCG 063; HYDRAZONE DERIVATIVE; LIPOPOLYSACCHARIDE; NITROIMIDAZOLE DERIVATIVE;

ADULT; ANTIINFLAMMATORY ACTIVITY; ARTICLE; CELL VIABILITY; CONTROLLED STUDY; CYTOKINE RELEASE; DRUG MECHANISM; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME PHOSPHORYLATION; FLUORESCENCE ACTIVATED CELL SORTING; GEL MOBILITY SHIFT ASSAY; HUMAN; HUMAN CELL; INFLAMMATION; MEDIATOR RELEASE; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; RETINA PIGMENT CELL; SIGNAL TRANSDUCTION; WESTERN BLOTTING; CELL LINE; CELL SURVIVAL; CYTOLOGY; DRUG EFFECTS; IMMUNOLOGY; MICROBIOLOGY; RETINAL PIGMENT EPITHELIUM;

ADULT; ANTI-INFLAMMATORY AGENTS; CELL LINE; CELL SURVIVAL; HUMANS; HYDRAZONES; INFLAMMATION; LIPOPOLYSACCHARIDES; NF-KAPPA B; NITROIMIDAZOLES; PROTO-ONCOGENE PROTEINS C-AKT; PSEUDOMONAS AERUGINOSA; REACTIVE OXYGEN SPECIES; RETINAL PIGMENT EPITHELIUM; SIGNAL TRANSDUCTION; TOLL-LIKE RECEPTOR 2;

EID: 84938064468     PISSN: 11073756     EISSN: 1791244X     Source Type: Journal    
DOI: 10.3892/ijmm.2015.2266     Document Type: Article

References (35)

1. Bertino JS Jr: Impact of antibiotic resistance in the management of ocular infections: The role of current and future antibiotics. Clin Ophthalmol 3: 507-521, 2009.
2. Al-Amri MS: Endogenous endophthalmitis secondary to pyogenic liver abscess. Int J Diabet Mellitus 2: 646-646, 2010.
3. Callegan MC, Engelbert M, Parke DW II, Jett BD and Gilmore MS: Bacterial endophthalmitis: Epidemiology, therapeutics, and bacterium-host interactions. Clin Microbiol Rev 15: 111-124, 2002.
4. Obritsch MD, Fish DN, MacLaren R and Jung R: Nosocomial infections due to multidrug-resistant Pseudomonas aeruginosa: Epidemiology and treatment options. Pharmacotherapy 25: 1353-1364, 2005.
5. Eifrig CW, Scott IU, Flynn HW Jr and Miller D: Endophthalmitis caused by Pseudomonas aeruginosa. Ophthalmology 110: 1714-1717, 2003.
6. Ren Y, Xie Y, Jiang G, Fan J, Yeung J, Li W, Tam PK and Savill J: Apoptotic cells protect mice against lipopolysaccharide-induced shock. J Immunol 180: 4978-4985, 2008.
7. Vallejo-Garcia JL, Asencio-Duran M, Pastora-Salvador N, Vinciguerra P and Romano MR: Role of inflammation in endophthalmitis. Mediators Inflamm 2012: 196094, 2012.
8. Campochiaro PA: Ocular neovascularization. J Mol Med Berl 91: 311-321, 2013.
9. Bonilha VL: Age and disease-related structural changes in the retinal pigment epithelium. Clin Ophthalmol 2: 413-424, 2008.
10. Arranz-Valsero I, Schulze U, Contreras-Ruiz L, García-Posadas L, López-García A, Paulsen F and Diebold Y: Involvement of corneal epithelial cells in the Th17 response in an in vitro bacterial inflammation model. Mol Vis 19: 85-99, 2013.
11. Gallucci RM, Simeonova PP, Matheson JM, Kommineni C, Guriel JL, Sugawara T and Luster MI: Impaired cutaneous wound healing in interleukin-6-deficient and immunosuppressed mice. FASEB J 14: 2525-2531, 2000.
12. Gallucci RM, Sugawara T, Yucesoy B, Berryann K, Simeonova PP, Matheson JM and Luster MI: Interleukin-6 treatment augments cutaneous wound healing in immunosuppressed mice. J Interferon Cytokine Res 21: 603-609, 2001.
13. Biswas PS, Banerjee K, Kinchington PR and Rouse BT: Involvement of IL-6 in the paracrine production of VEGF in ocular HSV-1 infection. Exp Eye Res 82: 46-54, 2006.
14. De Vos AF, Hoekzema R and Kijlstra A: Cytokines and uveitis, a review. Curr Eye Res 11: 581-597, 1992.
15. Fenton RR, Molesworth-Kenyon S, Oakes JE and Lausch RN: Linkage of IL-6 with neutrophil chemoattractant expression in virus-induced ocular inflammation. Invest Ophthalmol Vis Sci 43: 737-743, 2002.
16. Cole N, Bao S, Willcox M and Husband AJ: Expression of interleukin-6 in the cornea in response to infection with different strains of Pseudomonas aeruginosa. Infect Immun 67: 2497-2502, 1999.
17. Youker K, Smith CW, Anderson DC, Miller D, Michael LH, Rossen RD and Entman ML: Neutrophil adherence to isolated adult cardiac myocytes. Induction by cardiac lymph collected during ischemia and reperfusion. J Clin Invest 89: 602-609, 1992.
18. Liu Y, Shaw SK, Ma S, Yang L, Luscinskas FW and Parkos CA: Regulation of leukocyte transmigration: Cell surface interactions and signaling events. J Immunol 172: 7-13, 2004.
19. Tacke F and Randolph GJ: Migratory fate and differentiation of blood monocyte subsets. Immunobiology 211: 609-618, 2006.
20. Van Riper G, Siciliano S, Fischer PA, Meurer R, Springer MS and Rosen H: Characterization and species distribution of high affinity GTP-coupled receptors for human rantes and monocyte chemoattractant protein 1. J Exp Med 177: 851-856, 1993.
21. Carr MW, Roth SJ, Luther E, Rose SS and Springer TA: Monocyte chemoattractant protein 1 acts as a T-lymphocyte chemoattractant. Proc Natl Acad Sci USA 91: 3652-3656, 1994.
22. Kawashima A, Suzuki T, Nishihara F, Kobayashi T, Takaku Y, Nakagome K, Soma T, Hagiwara K, Kanazawa M and Nagata M: Effect of formoterol on eosinophil trans-basement membrane migration induced by interleukin-8-stimulated neutrophils.Int Arch Allergy Immunol 161 (Suppl 2): 10-15, 2013.
23. Chen YJ, Huang YS, Chen JT, Chen YH, Tai MC, Chen CL and Liang CM: Protective effects of glucosamine on oxidative-stress and ischemia/reperfusion-induced retinal injury. Invest Ophthalmol Vis Sci 56: 1506-1516, 2015.
24. Kim KH, Park JY, Jung HJ and Kwon HJ: Identification and biological activities of a new antiangiogenic small molecule that suppresses mitochondrial reactive oxygen species. Biochem Biophys Res Commun 404: 541-545, 2011.
25. Meng Z, Yan C, Deng Q, Gao DF and Niu XL: Curcumin inhibits LPS-induced inflammation in rat vascular smooth muscle cells in vitro via ROS-relative TLR4-MAPK/NF-κB pathways. Acta Pharmacol Sin 34: 901-911, 2013.
26. Novosad BD, Astley RA and Callegan MC: Role of Toll-like receptor (TLR) 2 in experimental Bacillus cereus endophthalmitis. PLoS One 6: e28619, 2011.
27. Schwarz JM and Bilbo SD: LPS elicits a much larger and broader inflammatory response than Escherichia coli infection within the hippocampus of neonatal rats. Neurosci Lett 497: 110-115, 2011.
28. Aderem A and Ulevitch RJ: Toll-like receptors in the induction of the innate immune response. Nature 406: 782-787, 2000.
29. Akira S and Takeda K: Toll-like receptor signalling. Nat Rev Immunol 4: 499-511, 2004.
30. Vogel SN, Fitzgerald KA and Fenton MJ: TLRs: Differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol Interv 3: 466-477, 2003.
31. McGuire VA, Gray A, Monk CE, Santos SG, Lee K, Aubareda A, Crowe J, Ronkina N, Schwermann J, Batty IH, et al: Cross talk between the Akt and p38α pathways in macrophages downstream of Toll-like receptor signaling. Mol Cell Biol 33: 4152-4165, 2013.
32. Jung WK, Lee DY, Park C, et al: Cilostazol is anti-inflammatory in BV2 microglial cells by inactivating nuclear factor-kappaB and inhibiting mitogen-activated protein kinases. Br J Pharmacol 159: 1274-1285, 2010.
33. Dąbek J, Kułach A and Gąsior Z: Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB): a new potential therapeutic target in atherosclerosis? Pharmacol Rep 62: 778-783, 2010.
34. Chen BC, Wu WT, Ho FM and Lin WW: Inhibition of interleukin-1beta-induced NF-kappa B activation by calcium/calmodulin-dependent protein kinase kinase occurs through Akt activation associated with interleukin-1 receptor-associated kinase phosphorylation and uncoupling of MyD88. J Biol Chem 277: 24169-24179, 2002.
35. Li W, Yan F, Zhou H, Lin X, Wu Y, Chen C, Zhou N, Chen Z, Li JD and Shen H: P. aeruginosa lipopolysaccharide-induced MUC5AC and CLCA3 expression is partly through Duox1 in vitro and in vivo. PLoS One 8: e63945, 2013.