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Volumn 42, Issue 6, 2015, Pages 915-924

Growth retardation of Escherichia coli by artificial increase of intracellular ATP

Author keywords

Growth retardation; High intracellular ATP concentration; Phosphoenolpyruvate carboxykinase; ROS scavenging

Indexed keywords

ADENOSINE TRIPHOSPHATE; DICARBOXYLATE TRANSPORTER; GLUTATHIONE; PHOSPHOENOLPYRUVATE CARBOXYLASE; REACTIVE OXYGEN METABOLITE; RHODOPSIN;

EID: 84937765396     PISSN: 13675435     EISSN: 14765535     Source Type: Journal    
DOI: 10.1007/s10295-015-1609-6     Document Type: Article
Times cited : (30)

References (37)
  • 2
    • 68049100110 scopus 로고    scopus 로고
    • Absolute metabolite concentrations and implied enzyme active site occupancy in Escherichia coli
    • COI: 1:CAS:528:DC%2BD1MXnslKqtLc%3D, PID: 19561621
    • Bennett BD, Kimball EH, Gao M, Osterhout R, Van Dien SJ, Rabinowitz JD (2009) Absolute metabolite concentrations and implied enzyme active site occupancy in Escherichia coli. Nat Chem Biol 5:593–599. doi:10.1038/nchembio.186
    • (2009) Nat Chem Biol , vol.5 , pp. 593-599
    • Bennett, B.D.1    Kimball, E.H.2    Gao, M.3    Osterhout, R.4    Van Dien, S.J.5    Rabinowitz, J.D.6
  • 3
    • 0037417864 scopus 로고    scopus 로고
    • Engineering the metabolism of Escherichia coli W3110 for the conversion of sugar to redox-neutral and oxidized products: homoacetate production
    • COI: 1:CAS:528:DC%2BD3sXhtF2gsr0%3D, PID: 12556564
    • Causey TB, Zhou S, Shanmugam KT, Ingram LO (2003) Engineering the metabolism of Escherichia coli W3110 for the conversion of sugar to redox-neutral and oxidized products: homoacetate production. Proc Natl Acad Sci USA 100:825–832. doi:10.1073/pnas.0337684100
    • (2003) Proc Natl Acad Sci USA , vol.100 , pp. 825-832
    • Causey, T.B.1    Zhou, S.2    Shanmugam, K.T.3    Ingram, L.O.4
  • 4
    • 0027377702 scopus 로고
    • Alteration of growth yield by overexpression of phosphoenolpyruvate carboxylase and phosphoenolpyruvate carboxykinase in Escherichia coli
    • COI: 1:CAS:528:DyaK2cXislansw%3D%3D, PID: 8285716
    • Chao YP, Liao JC (1993) Alteration of growth yield by overexpression of phosphoenolpyruvate carboxylase and phosphoenolpyruvate carboxykinase in Escherichia coli. Appl Environ Microbiol 59:4261–4265
    • (1993) Appl Environ Microbiol , vol.59 , pp. 4261-4265
    • Chao, Y.P.1    Liao, J.C.2
  • 5
    • 0028067589 scopus 로고
    • Metabolic responses to substrate futile cycling in Escherichia coli
    • COI: 1:CAS:528:DyaK2cXhvVOjsL8%3D, PID: 8106492
    • Chao YP, Liao JC (1994) Metabolic responses to substrate futile cycling in Escherichia coli. J Biol Chem 269:5122–5126
    • (1994) J Biol Chem , vol.269 , pp. 5122-5126
    • Chao, Y.P.1    Liao, J.C.2
  • 6
    • 84908577311 scopus 로고    scopus 로고
    • Cyanobacterial light-driven proton pump, Gloeobacter rhodopsin: complementarity between rhodopsin-based energy production and photosynthesis
    • PID: 25347537
    • Choi AR, Shi L, Brown LS, Jung K-H (2014) Cyanobacterial light-driven proton pump, Gloeobacter rhodopsin: complementarity between rhodopsin-based energy production and photosynthesis. PLoS ONE 9:e110643. doi:10.1371/journal.pone.0110643
    • (2014) PLoS ONE , vol.9 , pp. 110643
    • Choi, A.R.1    Shi, L.2    Brown, L.S.3    Jung, K.-H.4
  • 7
    • 0022410412 scopus 로고
    • Properties of an Escherichia coli mutant deficient in phosphoenolpyruvate carboxylase catalytic activity
    • COI: 1:CAS:528:DyaL2MXmtFSlsrY%3D, PID: 3902793
    • Coomes MW, Mitchell BK, Beezley A, Smith TE (1985) Properties of an Escherichia coli mutant deficient in phosphoenolpyruvate carboxylase catalytic activity. J Bacteriol 164:646–652
    • (1985) J Bacteriol , vol.164 , pp. 646-652
    • Coomes, M.W.1    Mitchell, B.K.2    Beezley, A.3    Smith, T.E.4
  • 8
    • 0032053161 scopus 로고    scopus 로고
    • Oxygen toxicity: a radical explanation
    • COI: 1:CAS:528:DyaK1cXjvVOns74%3D, PID: 9510531
    • Fridovich I (1998) Oxygen toxicity: a radical explanation. J Exp Biol 201:1203–1209
    • (1998) J Exp Biol , vol.201 , pp. 1203-1209
    • Fridovich, I.1
  • 9
    • 0021008839 scopus 로고
    • Phosphoenolpyruvate carboxylase of Escherichia coli. Inhibition by various analogs and homologs of phosphoenolpyruvate
    • COI: 1:CAS:528:DyaL2cXhtFOjsr4%3D, PID: 6368527
    • Izui K, Matsuda Y, Kameshita I, Katsuki H, Woods AE (1983) Phosphoenolpyruvate carboxylase of Escherichia coli. Inhibition by various analogs and homologs of phosphoenolpyruvate. J Biochem 94:1789–1795
    • (1983) J Biochem , vol.94 , pp. 1789-1795
    • Izui, K.1    Matsuda, Y.2    Kameshita, I.3    Katsuki, H.4    Woods, A.E.5
  • 11
    • 84866347141 scopus 로고    scopus 로고
    • Activity improvement of UDP-galactose-4-epimerase for tagatose substrates by 3D structure-based combinatorial mutagenesis
    • Kim HJ, Kang SY, Choi JH, Kim P (2012) Activity improvement of UDP-galactose-4-epimerase for tagatose substrates by 3D structure-based combinatorial mutagenesis. J Mol Catal B Enzym 84:35–39. doi:10.1016/j.molcatb.2012.04.020
    • (2012) J Mol Catal B Enzym , vol.84 , pp. 35-39
    • Kim, H.J.1    Kang, S.Y.2    Choi, J.H.3    Kim, P.4
  • 12
    • 84862860855 scopus 로고    scopus 로고
    • An engineered Escherichia coli having a high intracellular level of ATP and enhanced recombinant protein production
    • COI: 1:CAS:528:DC%2BC38XmtlSku7c%3D, PID: 22173482
    • Kim HJ, Kwon YD, Lee SY, Kim P (2012) An engineered Escherichia coli having a high intracellular level of ATP and enhanced recombinant protein production. Appl Microbiol Biotechnol 94:1079–1086. doi:10.1007/s00253-011-3779-0
    • (2012) Appl Microbiol Biotechnol , vol.94 , pp. 1079-1086
    • Kim, H.J.1    Kwon, Y.D.2    Lee, S.Y.3    Kim, P.4
  • 13
    • 2342516028 scopus 로고    scopus 로고
    • Effect of overexpression of Actinobacillus succinogenes phosphoenolpyruvate carboxykinase on succinate production in Escherichia coli
    • COI: 1:CAS:528:DC%2BD2cXhs1Cgsbc%3D, PID: 14766613
    • Kim P, Laivenieks M, Vieille C, Zeikus JG (2004) Effect of overexpression of Actinobacillus succinogenes phosphoenolpyruvate carboxykinase on succinate production in Escherichia coli. Appl Environ Microbiol 70:1238–1241. doi:10.1128/aem.70.2.1238-1241.2004
    • (2004) Appl Environ Microbiol , vol.70 , pp. 1238-1241
    • Kim, P.1    Laivenieks, M.2    Vieille, C.3    Zeikus, J.G.4
  • 14
    • 79953735971 scopus 로고    scopus 로고
    • Alteration of reducing powers in an isogenic phosphoglucose isomerase (pgi)-disrupted Escherichia coli expressing NAD(P)-dependent malic enzymes and NADP-dependent glyceraldehyde 3-phosphate dehydrogenase
    • COI: 1:CAS:528:DC%2BC3MXnt1ens78%3D, PID: 21272045
    • Kim S, Lee CH, Nam SW, Kim P (2011) Alteration of reducing powers in an isogenic phosphoglucose isomerase (pgi)-disrupted Escherichia coli expressing NAD(P)-dependent malic enzymes and NADP-dependent glyceraldehyde 3-phosphate dehydrogenase. Lett Appl Microbiol 52:433–440. doi:10.1111/j.1472-765X.2011.03013.x
    • (2011) Lett Appl Microbiol , vol.52 , pp. 433-440
    • Kim, S.1    Lee, C.H.2    Nam, S.W.3    Kim, P.4
  • 15
    • 58249110526 scopus 로고    scopus 로고
    • Comparison of the effects of NADH- and NADPH-perturbation stresses on the growth of Escherichia coli
    • COI: 1:CAS:528:DC%2BD1MXksVShtA%3D%3D, PID: 18953603
    • Kim S, Moon DB, Lee CH, Nam SW, Kim P (2009) Comparison of the effects of NADH- and NADPH-perturbation stresses on the growth of Escherichia coli. Curr Microbiol 58:159–163. doi:10.1007/s00284-008-9294-6
    • (2009) Curr Microbiol , vol.58 , pp. 159-163
    • Kim, S.1    Moon, D.B.2    Lee, C.H.3    Nam, S.W.4    Kim, P.5
  • 16
    • 0018874879 scopus 로고
    • The kinetic properties of phosphoenolpyruvate carboxykinase of Escherichia coli
    • COI: 1:CAS:528:DyaL3cXktVaqurk%3D, PID: 6991069
    • Krebs A, Bridger WA (1980) The kinetic properties of phosphoenolpyruvate carboxykinase of Escherichia coli. Can J Biochem 58:309–318. doi:10.1139/o80-041
    • (1980) Can J Biochem , vol.58 , pp. 309-318
    • Krebs, A.1    Bridger, W.A.2
  • 17
    • 0022528938 scopus 로고
    • Steady-state ATP synthesis by bacteriorhodopsin and chloroplast coupling factor co-reconstituted into asolectin vesicles
    • COI: 1:CAS:528:DyaL28XltFWnsLs%3D, PID: 2872676
    • Krupinski J, Hammes GG (1986) Steady-state ATP synthesis by bacteriorhodopsin and chloroplast coupling factor co-reconstituted into asolectin vesicles. Proc Natl Acad Sci USA 83:4233–4237
    • (1986) Proc Natl Acad Sci USA , vol.83 , pp. 4233-4237
    • Krupinski, J.1    Hammes, G.G.2
  • 18
    • 36549007021 scopus 로고    scopus 로고
    • The effect of NADP-dependent malic enzyme expression and anaerobic C4 metabolism in Escherichia coli compared with other anaplerotic enzymes
    • COI: 1:CAS:528:DC%2BD1cXnvFakug%3D%3D, PID: 18045419
    • Kwon YD, Kwon OH, Lee HS, Kim P (2007) The effect of NADP-dependent malic enzyme expression and anaerobic C4 metabolism in Escherichia coli compared with other anaplerotic enzymes. J Appl Microbiol 103:2340–2345. doi:10.1111/j.1365-2672.2007.03485.x
    • (2007) J Appl Microbiol , vol.103 , pp. 2340-2345
    • Kwon, Y.D.1    Kwon, O.H.2    Lee, H.S.3    Kim, P.4
  • 19
    • 33750196846 scopus 로고    scopus 로고
    • Influence of gluconeogenic phosphoenolpyruvate carboxykinase (PCK) expression on succinic acid fermentation in Escherichia coli under high bicarbonate condition
    • COI: 1:CAS:528:DC%2BD28Xht1Sqtr7K
    • Kwon YD, Lee SY, Kim P (2006) Influence of gluconeogenic phosphoenolpyruvate carboxykinase (PCK) expression on succinic acid fermentation in Escherichia coli under high bicarbonate condition. J Microbiol Biotechnol 16:1448–1452
    • (2006) J Microbiol Biotechnol , vol.16 , pp. 1448-1452
    • Kwon, Y.D.1    Lee, S.Y.2    Kim, P.3
  • 20
    • 43549095454 scopus 로고    scopus 로고
    • A physiology study of Escherichia coli overexpressing phosphoenolpyruvate carboxykinase
    • COI: 1:CAS:528:DC%2BD1cXls1Wqsb8%3D, PID: 18391462
    • Kwon YD, Lee SY, Kim P (2008) A physiology study of Escherichia coli overexpressing phosphoenolpyruvate carboxykinase. Biosci Biotechnol Biochem 72:1138–1141. doi:10.1271/bbb.70831
    • (2008) Biosci Biotechnol Biochem , vol.72 , pp. 1138-1141
    • Kwon, Y.D.1    Lee, S.Y.2    Kim, P.3
  • 21
    • 84878013603 scopus 로고    scopus 로고
    • Estimation of phosphoenolpyruvate carboxylation mediated by phosphoenolpyruvate carboxykinase (PCK) in engineered Escherichia coli having high ATP
    • COI: 1:CAS:528:DC%2BC3sXmsVGltL0%3D
    • Lee HJ, Kim HJ, Seo J, Na YA, Lee J, Lee JY, Kim P (2013) Estimation of phosphoenolpyruvate carboxylation mediated by phosphoenolpyruvate carboxykinase (PCK) in engineered Escherichia coli having high ATP. Enzyme Microbial Technol 53:13–17. doi:10.1016/j.enzmictec.2013.04.001
    • (2013) Enzyme Microbial Technol , vol.53 , pp. 13-17
    • Lee, H.J.1    Kim, H.J.2    Seo, J.3    Na, Y.A.4    Lee, J.5    Lee, J.Y.6    Kim, P.7
  • 22
    • 84899626307 scopus 로고    scopus 로고
    • Artificial oxidative stress-tolerant Corynebacterium glutamicum
    • PID: 24949252
    • Lee JY, Lee HJ, Seo J, Kim ES, Lee HS, Kim P (2014) Artificial oxidative stress-tolerant Corynebacterium glutamicum. AMB Express 4:15. doi:10.1186/s13568-014-0015-1
    • (2014) AMB Express , vol.4 , pp. 15
    • Lee, J.Y.1    Lee, H.J.2    Seo, J.3    Kim, E.S.4    Lee, H.S.5    Kim, P.6
  • 23
    • 80051594588 scopus 로고    scopus 로고
    • ATP regeneration system using E. coli ATP synthase and Gloeobacter rhodopsin and its stability
    • COI: 1:CAS:528:DC%2BC3MXnsF2iur0%3D, PID: 21780438
    • Lee KA, Jung K-H (2011) ATP regeneration system using E. coli ATP synthase and Gloeobacter rhodopsin and its stability. J Nanosci Nanotechnol 11:4261–4264. doi:10.1166/jnn.2011.3669
    • (2011) J Nanosci Nanotechnol , vol.11 , pp. 4261-4264
    • Lee, K.A.1    Jung, K.-H.2
  • 24
    • 84877319924 scopus 로고    scopus 로고
    • Effect of gene amplifications in porphyrin pathway on heme biosynthesis in a recombinant Escherichia coli
    • COI: 1:CAS:528:DC%2BC3sXhtVSiurbO, PID: 23648857
    • Lee MJ, Kim HJ, Lee JY, Kwon AS, Jun SY, Kang SH, Kim P (2013) Effect of gene amplifications in porphyrin pathway on heme biosynthesis in a recombinant Escherichia coli. J Microbiol Biotechnol 23:668–673
    • (2013) J Microbiol Biotechnol , vol.23 , pp. 668-673
    • Lee, M.J.1    Kim, H.J.2    Lee, J.Y.3    Kwon, A.S.4    Jun, S.Y.5    Kang, S.H.6    Kim, P.7
  • 25
    • 17344373614 scopus 로고    scopus 로고
    • Effect of oxidative stress caused by hydrogen peroxide on senescence of rice leaves
    • COI: 1:CAS:528:DyaK1cXlt1Wgsrk%3D
    • Lin J-N, Kao CH (1998) Effect of oxidative stress caused by hydrogen peroxide on senescence of rice leaves. Bot Bull Acad Sinica 39:161–165
    • (1998) Bot Bull Acad Sinica , vol.39 , pp. 161-165
    • Lin, J.-N.1    Kao, C.H.2
  • 26
    • 84885784009 scopus 로고    scopus 로고
    • Influence of foreign DNA introduction and periplasmic expression of recombinant human Interleukin-2 on hydrogen peroxide quantity and catalase activity in Escherichia coli
    • PID: 24312866
    • Mahmoudi Azar L, Mehdizadeh Aghdam E, Karimi F, Haghshenas B, Barzegari A, Yaghmaei P, Hejazi MS (2013) Influence of foreign DNA introduction and periplasmic expression of recombinant human Interleukin-2 on hydrogen peroxide quantity and catalase activity in Escherichia coli. Adv Pharm Bull 3:395–402. doi:10.5681/apb.2013.063
    • (2013) Adv Pharm Bull , vol.3 , pp. 395-402
    • Mahmoudi Azar, L.1    Mehdizadeh Aghdam, E.2    Karimi, F.3    Haghshenas, B.4    Barzegari, A.5    Yaghmaei, P.6    Hejazi, M.S.7
  • 27
    • 62649112114 scopus 로고    scopus 로고
    • The photocycle and proton translocation pathway in a cyanobacterial ion-pumping rhodopsin
    • COI: 1:CAS:528:DC%2BD1MXnvVersLs%3D, PID: 19217863
    • Miranda MR, Choi AR, Shi L, Bezerra AG Jr, Jung KH, Brown LS (2009) The photocycle and proton translocation pathway in a cyanobacterial ion-pumping rhodopsin. Biophys J 96:1471–1481. doi:10.1016/j.bpj.2008.11.026
    • (2009) Biophys J , vol.96 , pp. 1471-1481
    • Miranda, M.R.1    Choi, A.R.2    Shi, L.3    Bezerra, A.G.4    Jung, K.H.5    Brown, L.S.6
  • 28
    • 9644310554 scopus 로고    scopus 로고
    • Pyrroloquinoline-quinone: a reactive oxygen species scavenger in bacteria
    • COI: 1:CAS:528:DC%2BD2cXhtVClt7nM, PID: 15581610
    • Misra HS, Khairnar NP, Barik A, Indira Priyadarsini K, Mohan H, Apte SK (2004) Pyrroloquinoline-quinone: a reactive oxygen species scavenger in bacteria. FEBS Lett 578:26–30. doi:10.1016/j.febslet.2004.10.061
    • (2004) FEBS Lett , vol.578 , pp. 26-30
    • Misra, H.S.1    Khairnar, N.P.2    Barik, A.3    Indira Priyadarsini, K.4    Mohan, H.5    Apte, S.K.6
  • 29
    • 84883662198 scopus 로고    scopus 로고
    • WldS and PGC-1alpha regulate mitochondrial transport and oxidation state after axonal injury
    • O’Donnell KC, Vargas ME, Sagasti A (2013) WldS and PGC-1alpha regulate mitochondrial transport and oxidation state after axonal injury. J Neurosci Off J Soc Neurosci 33:14778–14790. doi:10.1523/JNEUROSCI.1331-13.2013
    • (2013) J Neurosci Off J Soc Neurosci , vol.33 , pp. 14778-14790
    • O’Donnell, K.C.1    Vargas, M.E.2    Sagasti, A.3
  • 30
    • 33750616739 scopus 로고    scopus 로고
    • L-arabinose isomerase from Geobacillus stearothermophilus for d-galactose isomerization
    • COI: 1:CAS:528:DC%2BD28Xht1Sgsb7K
    • L-arabinose isomerase from Geobacillus stearothermophilus for d-galactose isomerization. J Mol Catal B Enzym 43:108–112. doi:10.1016/j.molcatb.2006.06.015
    • (2006) J Mol Catal B Enzym , vol.43 , pp. 108-112
    • Oh, D.K.1    Oh, H.J.2    Kim, H.J.3    Cheon, J.4    Kim, P.5
  • 31
    • 37549000205 scopus 로고    scopus 로고
    • Multicopy suppression underpins metabolic evolvability
    • COI: 1:CAS:528:DC%2BD1cXnsVGitw%3D%3D, PID: 17884825
    • Patrick WM, Quandt EM, Swartzlander DB, Matsumura I (2007) Multicopy suppression underpins metabolic evolvability. Mol Biol Evol 24:2716–2722. doi:10.1093/molbev/msm204
    • (2007) Mol Biol Evol , vol.24 , pp. 2716-2722
    • Patrick, W.M.1    Quandt, E.M.2    Swartzlander, D.B.3    Matsumura, I.4
  • 32
    • 0030021703 scopus 로고    scopus 로고
    • Changes in the cellular energy state affect the activity of the bacterial phosphotransferase system
    • COI: 1:CAS:528:DyaK28Xotlahtg%3D%3D, PID: 8631333
    • Rohwer JM, Jensen PR, Shinohara Y, Postma PW, Westerhoff HV (1996) Changes in the cellular energy state affect the activity of the bacterial phosphotransferase system. Eur J Biochem 235:225–230
    • (1996) Eur J Biochem , vol.235 , pp. 225-230
    • Rohwer, J.M.1    Jensen, P.R.2    Shinohara, Y.3    Postma, P.W.4    Westerhoff, H.V.5
  • 33
    • 54349122238 scopus 로고    scopus 로고
    • Mitochondria-dependent reactive oxygen species-mediated programmed cell death induced by 3,3′-diindolylmethane through inhibition of F0F1-ATP synthase in unicellular protozoan parasite Leishmania donovani
    • COI: 1:CAS:528:DC%2BD1cXhtlCntrfI, PID: 18703668
    • Roy A, Ganguly A, BoseDasgupta S, Das BB, Pal C, Jaisankar P, Majumder HK (2008) Mitochondria-dependent reactive oxygen species-mediated programmed cell death induced by 3,3′-diindolylmethane through inhibition of F0F1-ATP synthase in unicellular protozoan parasite Leishmania donovani. Mol Pharmacol 74:1292–1307. doi:10.1124/mol.108.050161
    • (2008) Mol Pharmacol , vol.74 , pp. 1292-1307
    • Roy, A.1    Ganguly, A.2    BoseDasgupta, S.3    Das, B.B.4    Pal, C.5    Jaisankar, P.6    Majumder, H.K.7
  • 35
    • 56649093519 scopus 로고    scopus 로고
    • Hydrogen peroxide induces oxidative stress in detached leaves of Oryza sativa L
    • COI: 1:CAS:528:DC%2BD1MXltFamsw%3D%3D
    • Upadhyaya H, Khan MH, Panda SK (2007) Hydrogen peroxide induces oxidative stress in detached leaves of Oryza sativa L. Gen Appl Plant Physiol 33:83–95
    • (2007) Gen Appl Plant Physiol , vol.33 , pp. 83-95
    • Upadhyaya, H.1    Khan, M.H.2    Panda, S.K.3
  • 36
    • 0141504947 scopus 로고    scopus 로고
    • Analysis of Escherichia coli anaplerotic metabolism and its regulation mechanisms from the metabolic responses to altered dilution rates and phosphoenolpyruvate carboxykinase knockout
    • PID: 12966569
    • Yang C, Hua Q, Baba T, Mori H, Shimizu K (2003) Analysis of Escherichia coli anaplerotic metabolism and its regulation mechanisms from the metabolic responses to altered dilution rates and phosphoenolpyruvate carboxykinase knockout. Biotechnol Bioeng 84:129–144. doi:10.1002/bit.10692
    • (2003) Biotechnol Bioeng , vol.84 , pp. 129-144
    • Yang, C.1    Hua, Q.2    Baba, T.3    Mori, H.4    Shimizu, K.5
  • 37
    • 33644552417 scopus 로고    scopus 로고
    • Increased production of reactive oxygen species in hyperglycemic conditions requires dynamic change of mitochondrial morphology
    • COI: 1:CAS:528:DC%2BD28XksF2rsbk%3D, PID: 16477035
    • Yu T, Robotham JL, Yoon Y (2006) Increased production of reactive oxygen species in hyperglycemic conditions requires dynamic change of mitochondrial morphology. Proc Nat Acad Sci USA 103:2653–2658. doi:10.1073/pnas.0511154103
    • (2006) Proc Nat Acad Sci USA , vol.103 , pp. 2653-2658
    • Yu, T.1    Robotham, J.L.2    Yoon, Y.3


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