Estimation of phosphoenolpyruvate carboxylation mediated by phosphoenolpyruvate carboxykinase (PCK) in engineered Escherichia coli having high ATP

Enzyme and Microbial Technology

Volumn 53, Issue 1, 2013, Pages 13-17

  Lee, Hyo Jung ^a   Kim, Hye Jung ^a,b   Seo, Jiyoon ^a,c   Na, Yoon Ah ^a   Lee, Jiyeon ^a   Lee, Joo Young ^a   Kim, Pil ^a  

^a The Catholic University of Korea   (South Korea)

^b Samyang Corporation   (South Korea)

^c ST Pharm Co Ltd   (South Korea)

Author keywords

Enzyme kinetics; High intracellular ATP concentration; Phosphoenolpyruvate carboxylase (PPC); Phosphoenolpyruvate carboxylkinase (PCK)

Indexed keywords

ALLOSTERIC EFFECTORS; CATALYTIC EFFICIENCIES; ENGINEERED ESCHERICHIA COLI; FRUCTOSE 1 ,6-BISPHOSPHATE; INTRACELLULAR ATP; PHOSPHOENOLPYRUVATE CARBOXYKINASE; PHOSPHOENOLPYRUVATE CARBOXYLASE; PHOSPHOENOLPYRUVATES;

ENZYME KINETICS; ESCHERICHIA COLI; PLANTS (BOTANY); RECOMBINANT PROTEINS;

CARBOXYLATION;

ADENOSINE TRIPHOSPHATE; PHOSPHOENOLPYRUVATE CARBOXYKINASE (GTP); PHOSPHOENOLPYRUVATE CARBOXYLASE; RECOMBINANT ENZYME; PHOSPHOENOLPYRUVATE CARBOXYKINASE (ATP); RECOMBINANT PROTEIN;

ALLOSTERISM; ARTICLE; BACTERIAL STRAIN; CARBOXYLATION; CATALYSIS; CHROMOSOME; ESCHERICHIA COLI; NONHUMAN; NUCLEOTIDE SEQUENCE; PROMOTER REGION; BIOTECHNOLOGY; ENZYMOLOGY; EVALUATION STUDY; GENETIC ENGINEERING; GENETICS; KINETICS; METABOLISM; PROCEDURES;

ESCHERICHIA COLI;

ADENOSINE TRIPHOSPHATE; BIOTECHNOLOGY; ESCHERICHIA COLI; GENETIC ENGINEERING; KINETICS; PHOSPHOENOLPYRUVATE CARBOXYKINASE (ATP); PHOSPHOENOLPYRUVATE CARBOXYLASE; RECOMBINANT PROTEINS;

EID: 84878013603     PISSN: 01410229     EISSN: 18790909     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2013.04.001     Document Type: Article

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