Rapid transposon liquid enrichment sequencing (TnLE-seq) for gene fitness evaluation in underdeveloped bacterial systems

Applied and Environmental Microbiology

Volumn 79, Issue 23, 2013, Pages 7510-7517

  Fels, Samuel R ^a   Zane, Grant M ^b   Blake, Sean M ^a,c   Wall, Judy D ^a,b  

^a UNIVERSITY OF MISSOURI   (United States)

^b University of Missouri   (United States)

^c Global Biologics   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANAEROBIC BACTERIUM; DESULFOVIBRIO VULGARIS; FITNESS EVALUATIONS; LABORATORY CONDITIONS; MASSIVELY PARALLEL SEQUENCING; SIMULTANEOUS ANALYSIS; TRANSPOSON INSERTION SITE; TRANSPOSON MUTAGENESIS;

BACTERIA; GROWTH KINETICS; HEALTH; LIQUIDS;

GENES;

BACTERIUM; ELECTRON; FITNESS; GENE EXPRESSION; GROWTH RATE; MUTATION; REACTION KINETICS; RESOLUTION;

LACTIC ACID DERIVATIVE; SULFATE;

ANAEROBIC GROWTH; ARTICLE; BACTERIUM CONJUGATION; DESULFOVIBRIO VULGARIS; DNA SEQUENCE; GENE DELETION; GENE EXPRESSION REGULATION; GENETIC SELECTION; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; METHODOLOGY; MICROBIAL GENETICS; MOLECULAR BIOLOGY; TRANSPOSON;

ANAEROBIOSIS; CONJUGATION, GENETIC; DESULFOVIBRIO VULGARIS; DNA TRANSPOSABLE ELEMENTS; GENE DELETION; GENETICS, MICROBIAL; LACTATES; MOLECULAR BIOLOGY; MUTAGENESIS, INSERTIONAL; SELECTION, GENETIC; SEQUENCE ANALYSIS, DNA; SULFATES;

EID: 84888268881     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02051-13     Document Type: Article

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