Molecular Identification and Quantification of Tetracycline and Erythromycin Resistance Genes in Spanish and Italian Retail Cheeses

BioMed Research International

Volumn 2014, Issue , 2014, Pages

  Flórez, Ana Belén ^a   Alegría, Ángel ^a,b   Rossi, Franca ^b   Delgado, Susana ^a   Felis, Giovanna E ^b   Torriani, Sandra ^b   Mayo, Baltasar ^a  

^a DEPARTMENT OF MICROBIOLOGY AND BIOCHEMISTRY OF DAIRY PRODUCTS   (Spain)

^b UNIVERSITY OF VERONA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

DNA 16S; ERYTHROMYCIN; TETRACYCLINE; ANTIINFECTIVE AGENT; BACTERIAL PROTEIN;

AMPLICON; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL COUNT; BACTERIAL GENE; BACTERIAL LOAD; CHEESE; CONTROLLED STUDY; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA EXTRACTION; DNA PURIFICATION; DNA TEMPLATE; ERM B GENE; ERM F GENE; GENE DOSAGE; GENE IDENTIFICATION; GENETIC VARIABILITY; LIMIT OF DETECTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PLATE COUNT; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; TET K GENE; TET L GENE; TET M GENE; TET O GENE; TET S GENE; TET W GENE; ANALYSIS; BACTERIUM; DRUG EFFECTS; FOOD ANALYSIS; FOOD CONTROL; FOOD INDUSTRY; ISOLATION AND PURIFICATION; ITALY; METABOLISM; MICROBIOLOGY; PHYSIOLOGY;

BACTERIA (MICROORGANISMS);

ANTI-BACTERIAL AGENTS; BACTERIA; BACTERIAL PROTEINS; CHEESE; DRUG RESISTANCE, BACTERIAL; ERYTHROMYCIN; FOOD ANALYSIS; FOOD INDUSTRY; FOOD MICROBIOLOGY; ITALY; TETRACYCLINE;

EID: 84935911500     PISSN: 23146133     EISSN: 23146141     Source Type: Journal    
DOI: 10.1155/2014/746859     Document Type: Article

References (44)

1. J. Davies and D. Davies, "Origins and evolution of antibiotic resistance, "Microbiology andMolecular Biology Reviews, vol. 74, no. 3, pp. 417-433, 2010.
2. V. M. DCosta, C. E. King, L. Kalan et al., "Antibiotic resistance is ancient," Nature, vol. 477, no. 7365, pp. 457461, 2011.
3. G. D. Wright, "The antibiotic resistome: the nexus of chemical and genetic diversity, " Nature Reviews Microbiology, vol. 5, no. 3, pp. 175-186, 2007.
4. S. B. Levy and B. Marshall, "Antibacterial resistance worldwide: causes, challenges and responses, " Nature Medicine, vol. 10, supplement 12, pp. S122-S129, 2004.
5. A. H. A. M. van Hoek, D. Mevius, B. Guerra, P. Mullany, A. P. Roberts, and H. J. M. Aarts, "Acquired antibiotic resistance genes: an overview, " Frontiers inMicrobiology, vol. 2, article 203, 2011.
6. D. J. Donoghue, "Antibiotic residues in poultry tissues and eggs: human health concerns?" Poultry Science, vol. 82, no. 4, pp. 618-621, 2003.
7. G. M. Duran and D. L. Marshall, "Ready-to-eat shrimp as an international vehicle of antibiotic-resistant bacteria, " Journal of Food Protection, vol. 68, no. 11, pp. 2395-2401, 2005.
8. H. H. Wang, M. Manuzon, M. Lehman et al., "Food commensal microbes as a potentially important avenue in transmitting antibiotic resistance genes, " FEMSMicrobiology Letters, vol. 254, no. 2, pp. 226-231, 2006.
9. X. Li and H. H. Wang, "Tetracycline resistance associated with commensal bacteria fromrepresentative ready-to-consume deli and restaurant foods, " Journal of Food Protection, vol. 73, no. 10, pp. 1841-1848, 2010.
10. V. Perreten, F. Schwarz, L. Cresta, M. Boeglin, G. Dasen, andM. Teuber, "Antibiotic resistance spread in food, " Nature, vol. 389, no. 6653, pp. 801-802, 1997.
11. K. P. Scott, C. M. Melville, T. M. Barbosa, andH. J. Flint, "Occurrence of the new tetracycline resistance gene tet (W) in bacteria from the human gut, " Antimicrobial Agents and Chemotherapy, vol. 44, no. 3, pp. 775-777, 2000.
12. M. S. Ammor, A. B. Florez, A. H. A. M. vanHoek et al., "Molecular characterization of intrinsic and acquired antibiotic resistance in lactic acid bacteria and bifidobacteria, " Journal of Molecular Microbiology and Biotechnology, vol. 14, pp. 6-15, 2008.
13. M. S. Smith, R. K. Yang, C. W. Knapp et al., "Quantification of tetracycline resistance genes in feedlot lagoons by real-time PCR, " Applied and Environmental Microbiology, vol. 70, no. 12, pp. 7372-7377, 2004.
14. M. Y. Manuzon, S. E. Hanna, H. Luo, Z. Yu, W. J. Harper, and H. H. Wang, "Quantitative assessment of the tetracycline resistance gene pool in cheese samples by real-time TaqMan PCR, " Applied and Environmental Microbiology, vol. 73, no. 5, pp. 1676-1677, 2007.
15. R. I. Aminov, N. Garrigues-Jeanjean, and R. I. Mackie, "Molecular ecology of tetracycline resistance: development and validation of primers for detection of tetracycline resistance genes encoding ribosomal protection proteins, " Applied and Environmental Microbiology, vol. 67, no. 1, pp. 22-32, 2001.
16. J. C. Chee-Sanford, R. I. Aminov, I. J. Krapac, N. Garrigues-Jeanjean, and R. I. Mackie, "Occurrence and diversity of tetracycline resistance genes in lagoons and groundwater underlying two swine production facilities, " Applied and Environmental Microbiology, vol. 67, no. 4, pp. 1494-1502, 2001.
17. Z. Yu, F. C. Michel Jr., G. Hansen, T. Wittum, and M. Morrison, "Development and application of real-time PCR assays for quantification of genes encoding tetracycline resistance, " Applied and Environmental Microbiology, vol. 71, no. 11, pp. 6926-6933, 2005.
18. M. L. Diaz-Torres, R. McNab, D. A. Spratt et al., "Novel tetracycline resistance determinant from the oral metagenome, " Antimicrobial Agents and Chemotherapy, vol. 47, no. 4, pp. 1430-1432, 2003.
19. L. A. Seville, A. J. Patterson, K. P. Scott et al., "Distribution of tetracycline and erythromycin resistance genes among human oral and fecal metagenomic DNA, " Microbial Drug Resistance, vol. 15, no. 3, pp. 159-166, 2009.
20. M. O. A. Sommer, G. Dantas, and G. M. Church, "Functional characterization of the antibiotic resistance reservoir in the human microflora, " Science, vol. 325, no. 5944, pp. 1128-1131, 2009.
21. A. J. Patterson, R. Colangeli, P. Spigaglia, and K. P. Scott, "Distribution of specific tetracycline and erythromycin resistance genes in environmental samples assessed by macroarray detection, " Environmental Microbiology, vol. 9, no. 3, pp. 703-715, 2007.
22. S. Delgado, F. Fracchetti, B. Mayo, and S. Torriani, "Development and validation of amultiplex PCR-basedDNAmicroarray hybridisation method for detecting bacterial antibiotic resistance genes in cheese, " International Dairy Journal, vol. 21, no. 3, pp. 149-157, 2011.
23. K. J. Forsberg, A. Reyes, B. Wang, E. M. Selleck, M. O. A. Sommer, and G. Dantas, "Theshared antibiotic resistome of soil bacteria and human pathogens, " Science, vol. 337, no. 6098, pp. 1107-1111, 2012.
24. P. Mullany, E. Allan, and P. J. Warburton, "Tetracycline resistance genes and mobile genetic elements from the oral metagenome, " Clinical Microbiology and Infection, vol. 18, no. 4, pp. 58-61, 2012.
25. T. M. Barbosa, K. P. Scott, and H. J. Flint, "Evidence for recent intergeneric transfer of a new tetracycline resistance gene, tet(W), isolated from Butyrivibrio fibrisolvens, and the occurrence of tet(O) in ruminai bacteria, " EnvironmentalMicrobiology, vol. 1, no. 1, pp. 53-64, 1999.
26. D. Gevers, G. Huys, and J. Swings, "In vitro conjugal transfer of tetracycline resistance from Lactobacillus isolates to other gram-positive bacteria, " FEMS Microbiology Letters, vol. 225, no. 1, pp. 125-130, 2003.
27. M. C. Roberts, W. O. Chung, D. Roe et al., "Erythromycinresistant Neisseria gonorrhoeae and oral commensal Neisseria spp. carry known rRNAmethylase genes, " Antimicrobial Agents and Chemotherapy, vol. 43, no. 6, pp. 1367-1372, 1999.
28. S. E. Denman and C. S. McSweeney, "Development of a realtime PCR assay formonitoring anaerobic fungal and cellulolytic bacterial populations within the rumen, " FEMS Microbiology Ecology, vol. 58, no. 3, pp. 572-582, 2006.
29. A. B. Florez, S. Delgado, and B. Mayo, "Antimicrobial susceptibility of lactic acid bacteria isolated froma cheese environment, " Canadian Journal ofMicrobiology, vol. 51, no. 1, pp. 51-58, 2005.
30. A. B. Florez and B. Mayo, "Microbial diversity and succession during the manufacture and ripening of traditional, Spanish, blue-veined Cabrales cheese, as determined by PCR-DGGE, " International Journal of Food Microbiology, vol. 110, no. 2, pp. 165-171, 2006.
31. A. B. Florez, M. S. Ammor, P. Alvarez-Martin, A. Margolles, and B. Mayo, "Molecular analysis of tet(W) gene-mediated tetracycline resistance in dominant intestinal Bifidobacterium species from healthy humans, " Applied and Environmental Microbiology, vol. 72, no. 11, pp. 7377-7379, 2006.
32. A. A. Salyers, A. Gupta, and Y. Wang, "Human intestinal bacteria as reservoirs for antibiotic resistance genes, " Trends in Microbiology, vol. 12, no. 9, pp. 412-416, 2004.
33. C. Devirgiliis, A. Caravelli, D. Coppola, S. Barile, and G. Perozzi, "Antibiotic resistance and microbial composition along the manufacturing process of Mozzarella di Bufala Campana, " International Journal of Food Microbiology, vol. 128, no. 2, pp. 378-384, 2008.
34. C. L. Cain, "Antimicrobial resistance in staphylococci in small animals, " Veterinary Clinics of North America: Small Animal Practice, vol. 43, no. 1, pp. 19-40, 2013.
35. C. Zhao, H. Sun, H. Wang et al., "Antimicrobial resistance trends among 5608 clinical Gram-positive isolates in China: results fromthe Gram-Positive Cocci Resistance Surveillance program (2005-2010), " Diagnostic Microbiology and Infectious Disease, vol. 73, no. 2, pp. 174-181, 2012.
36. L. L. McGowan-Spicer, P. J. Fedorka-Cray, J. G. Frye, R. J. Meinersmann, J. B. Barrett, and C. R. Jackson, "Antimicrobial resistance and virulence of Enterococcus faecalis isolated from retail food, " Journal of Food Protection, vol. 71, no. 4, pp. 760-769, 2008.
37. X. Li, Y. Li, V. Alvarez, W. J. Harper, and H. H. Wang, "Effective antibiotic resistance mitigation during cheese fermentation, " Applied and Environmental Microbiology, vol. 77, no. 20, pp. 7171-7175, 2011.
38. H. H. Wang and D. W. Schaffner, "Antibiotic resistance: how much do we know and where do we go fromhere?" Applied and EnvironmentalMicrobiology, vol. 77, no. 20, pp. 7093-7095, 2011.
39. J. Chen, Z. Yu, F. C. Michel Jr., T. Wittum, and M. Morrison, "Development and application of real-time PCR assays for quantification of ermgenes conferring resistance to macrolideslincosamides-streptogramin B in livestock manure andmanure management systems, " Applied and Environmental Microbiology, vol. 73, no. 14, pp. 4407-4416, 2007.
40. S.-M. Kim, H. C. Kim, and S.-W. S. Lee, "Characterization of antibiotic resistance determinants in oral biofilms, " Journal of Microbiology, vol. 49, no. 4, pp. 595-602, 2011.
41. L. Zhang, D. Kinkelaar, Y. Huang, Y. Li, X. Li, and H. H. Wang, "Acquired antibiotic resistance: are we born with it?" Applied and Environmental Microbiology, vol. 77, no. 20, pp. 7134-7141, 2011.
42. M. Guarddon, J. M. Miranda, J. A. Rodriguez, B. I. Vazquez, A. Cepeda, and C. M. Franco, "Real-time polymerase chain reaction for the quantitative detection of tetA and tetB bacterial tetracycline resistance genes in food, " International Journal of Food Microbiology, vol. 146, no. 3, pp. 284-289, 2011.
43. L. Feld, S. Schjorring, K. Hammer et al., "Selective pressure affects transfer and establishment of a Lactobacillus plantarum resistance plasmid in the gastrointestinal environment, " Journal of Antimicrobial Chemotherapy, vol. 61, no. 4, pp. 845-852, 2008.
44. L. Jacobsen, A. Wilcks, K. Hammer, G. Huys, D. Gevers, and S. R. Andersen, "Horizontal transfer of tet(M) and erm(B) resistance plasmids from food strains of Lactobacillus plantarum to Enterococcus faecalis JH2-2 in the gastrointestinal tract of gnotobiotic rats, " FEMS Microbiology and Ecology, vol. 59, no. 1, pp. 158-166, 2007.