Deep sequencing reveals different compositions of mRNA transcribed from the F8 gene in a panel of FVIII-producing CHO cell lines

Biotechnology Journal

Volumn 10, Issue 7, 2015, Pages 1081-1089

  Kaas, Christian S ^a,b   Bolt, Gert ^a   Hansen, Jens J ^a   Andersen, Mikael R ^b   Kristensen, Claus ^a,c  

^a NOVO NORDISK A S   (Denmark)

^b TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^c UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

Cell culture; CHO cells; Coagulation factor VIII; Gene delivery; Next generation sequencing

Indexed keywords

CELL CULTURE; CELLS; COAGULATION; CYTOLOGY; GENE TRANSFER; GENES; MAMMALS; NUCLEIC ACIDS; PRODUCTIVITY; RNA;

BIOPHARMACEUTICALS; CHO CELL; COAGULATION FACTOR; GENE DELIVERY; MICROARRAY TECHNOLOGIES; NEXT-GENERATION SEQUENCING; POST-TRANSLATIONAL MODIFICATIONS; THREE ORDERS OF MAGNITUDE;

CLONE CELLS;

BLOOD CLOTTING FACTOR 8; MESSENGER RNA; F8 PROTEIN, HUMAN; RECOMBINANT PROTEIN; TRANSCRIPTOME;

ARTICLE; CELL CLONE; CHO CELL LINE; CONTROLLED STUDY; F8 GENE; GENE; HUMAN; HUMAN CELL; INTERNAL RIBOSOME ENTRY SITE; MOLECULAR CLONING; NEXT GENERATION SEQUENCING; PRIORITY JOURNAL; PRODUCTIVITY; PROMOTER REGION; RNA ANALYSIS; RNA SEQUENCE; TRANSGENE; ANIMAL; BIOSYNTHESIS; CRICETULUS; GENETICS; HAMSTER; HIGH THROUGHPUT SEQUENCING; PROTEIN PROCESSING;

MAMMALIA;

ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; FACTOR VIII; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT PROTEINS; RNA, MESSENGER; TRANSCRIPTOME;

EID: 84935749233     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201400667     Document Type: Article

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