Neuroprotective effects of quercetin in a mouse model of brain ischemic/reperfusion injury via anti-apoptotic mechanisms based on the Akt pathway

Molecular Medicine Reports

Volumn 12, Issue 3, 2015, Pages 3688-3696

  Lei, Xiaoming ^a   Chao, Hailian ^a   Zhang, Zhenni ^a   Lv, Jianrui ^a   Li, Siyuan ^a   Wei, Haidong ^a   Xue, Rongliang ^a   Li, Fang ^a   Li, Zongfang ^a  

^a XI'AN JIAOTONG UNIVERSITY   (China)

Author keywords

Akt; Global cerebral ischemia reperfusion; Neuroprotection; Quercetin; Reactive oxygen species

Indexed keywords

CASPASE 3; PROTEIN BCL 2; PROTEIN BCL XL; PROTEIN KINASE B; QUERCETIN; REACTIVE OXYGEN METABOLITE; SURVIVIN; NEUROPROTECTIVE AGENT;

ANIMAL EXPERIMENT; ANIMAL MODEL; APOPTOSIS; ARTICLE; BRAIN ISCHEMIA; BRAIN NERVE CELL; CELL LOSS; CONTROLLED STUDY; DRUG DOSE INCREASE; FLUORESCENCE ACTIVATED CELL SORTING; HIPPOCAMPAL CA1 REGION; LONG TERM MEMORY; MALE; NEUROPROTECTION; NONHUMAN; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; RAT; REPERFUSION INJURY; SIGNAL TRANSDUCTION; SPATIAL LEARNING; TUNEL ASSAY; UPREGULATION; WESTERN BLOTTING; ANIMAL; BRAIN; DRUG EFFECTS; MEMORY; METABOLISM; NERVE CELL; PATHOLOGY; SPRAGUE DAWLEY RAT;

RATTUS;

ANIMALS; APOPTOSIS; BRAIN; BRAIN ISCHEMIA; MALE; MEMORY; NEURONS; NEUROPROTECTIVE AGENTS; PROTO-ONCOGENE PROTEINS C-AKT; QUERCETIN; RATS, SPRAGUE-DAWLEY; REACTIVE OXYGEN SPECIES; REPERFUSION INJURY; SIGNAL TRANSDUCTION; SPATIAL LEARNING;

EID: 84935423550     PISSN: 17912997     EISSN: 17913004     Source Type: Journal    
DOI: 10.3892/mmr.2015.3857     Document Type: Article

References (38)

1. Cherubini A, Ruggiero C, Morand C, et al: Dietary antioxidants as potential pharmacological agents for ischemic stroke. Curr Med Chem 15:1236-1248, 2008.
2. Harwood M, Danielewska-Nikiel B, Borzelleca JF, Flamm GW, Williams GM and Lines TC: A critical review of the data related to the safety of quercetin and lack of evidence of in vivo toxicity, including lack of genotoxic/carcinogenic properties. Food Chem Toxicol 45:2179-2205, 2007.
3. Lee JC, Kim J, Park JK, Chung GH and Jang YS: The antioxidant, rather than prooxidant, activities of quercetin on normal cells: Quercetin protects mouse thymocytes from glucose oxidase-mediated apoptosis. Exp Cell Res 291:386-397, 2003.
4. Lapi D, Vagnani S, Pignataro G, esposito E, Paterni M and Colantuoni A: Protective effects of Quercetin on Rat Pial microvascular changes during transient bilateral common carotid artery occlusion and reperfusion. Front Physiol 3:32, 2012.
5. Ghosh A, Sarkar S, Mandal AK and Das N: Neuroprotective role of nanoencapsulated quercetin in combating ischemia-reperfusion induced neuronal damage in young and aged rats. PLoS One 8:e57735, 2013.
6. Mattson MP, Duan W, Pedersen WA and Culmsee C: Neurodegenerative disorders and ischemic brain diseases. Apoptosis 6:69-81, 2001.
7. Kowaltowski AJ, Castilho RF and Vercesi AE: Mitochondrial permeability transition and oxidative stress. FEBS Lett 495:12-15, 2001.
8. Kuwana T, Mackey MR, Perkins G, et al: Bid, Bax and lipids cooperate to form supramolecular openings in the outer mitochondrial membrane. Cell 111:331-342, 2002.
9. Lei C, Deng J, Wang B, et al: Reactive oxygen species scavenger inhibits STAT3 activation after transient focal cerebral ischemia-reperfusion injury in rats. Anesth Analg 113:153-159, 2011.
10. Priyadarsini RV and Nagini S: Quercetin suppresses cytochrome P450 mediated ROS generation and NF kappaB activation to inhibit the development of 7, 12-dimethylbenz a anthracene (DMBA) induced hamster buccal pouch carcinomas. Free Radic Res 46:41-49, 2012.
11. Yao RQ, Qi DS, Yu HL, Liu J, Yang LH and Wu XX: Quercetin attenuates cell apoptosis in focal cerebral ischemia rat brain via activation of BDNF-TrkB-PI3K/Akt signaling pathway. Neurochem Res 37:2777-2786, 2012.
12. Mayo LD and Donner DB: A phosphatidylinositol 3-kinase/Akt pathway promotes translocation of Mdm2 from the cytoplasm to the nucleus. Proc Natl Acad Sci USA 98:11598-11603, 2001.
13. Janelidze S, Hu BR, Siesjo P and Siesjo BK: Alterations of Akt1 (PKBalpha) and p70 (S6K) in transient focal ischemia. Neurobiol Dis 8:147-154, 2001.
14. Shibata M, Yamawaki T, Sasaki T, et al: Upregulation of Akt phosphorylation at the early stage of middle cerebral artery occlusion in mice. Brain Res 942:1-10, 2002.
15. Noshita N, Lewen A, Sugawara T and Chan PH: Evidence of phosphorylation of Akt and neuronal survival after transient focal cerebral ischemia in mice. J Cereb Blood Flow Metab 21:1442-1450, 2001.
16. Xue RL, He JX, Wang N, Yao FZ, Lv JR and Wu G: Relationship between transmembrane signal transduction pathway and DNA repair and the mechanism after global cerebral ischemia-reperfusion in rats. Neurosci Bull 25:115-121, 2009.
17. Hadley G, Papadakis M and Buchan AM: A method of inducing global cerebral ischemia. Methods Mol Biol 1135:111-120, 2014.
18. Geocadin RG, Ghodadra R, Kimura T, et al: A novel quantitative EEG injury measure of global cerebral ischemia. Clin Neurophysiol 111:1779-1787, 2000.
19. Vorhees CV and Williams MT: Morris water maze: procedures for assessing spatial and related forms of learning and memory. Nat Protoc 1:848-858, 2006.
20. Stackman RW Jr, Lora JC and Williams SB: Directional responding of C57BL/6J mice in the Morris water maze is influenced by visual and vestibular cues and is dependent on the anterior thalamic nuclei. J Neurosci 32:10211-10225, 2012.
21. Simao F, Matte A, Matte C, et al: Resveratrol prevents oxidative stress and inhibition of Na (+) K (+)-ATPase activity induced by transient global cerebral ischemia in rats. J Nutr Biochem 22:921-928, 2011.
22. Johansson SE, Larsen SS, Povlsen GK and Edvinsson L: Early MEK1/2 inhibition after global cerebral ischemia in rats reduces brain damage and improves outcome by preventing delayed vasoconstrictor receptor upregulation. PLoS One 9:e92417, 2014.
23. Ahmad A, Khan MM, Hoda MN, et al: Quercetin protects against oxidative stress associated damages in a rat model of transient focal cerebral ischemia and reperfusion. Neurochem Res 36:1360-1371, 2011.
24. Pandey AK, Hazari PP, Patnaik R and Mishra AK: The role of ASIC1a in neuroprotection elicited by quercetin in focal cerebral ischemia. Brain Res 1383:289-299, 2011.
25. Rizk NN, Rafols JA and Dunbar JC: Cerebral ischemia-induced apoptosis and necrosis in normal and diabetic rats: effects of insulin and C-peptide. Brain Res 1096:204-212, 2006.
26. Solaroglu I, Tsubokawa T, Cahill J and Zhang JH: Anti-apoptotic effect of granulocyte-colony stimulating factor after focal cerebral ischemia in the rat. Neuroscience 143:965-974, 2006.
27. Yousuf S, Atif F, Ahmad M, et al: Resveratrol exerts its neuroprotective effect by modulating mitochondrial dysfunctions and associated cell death during cerebral ischemia. Brain Res 1250:242-253, 2009.
28. Wang R, Li G, Wang W and Li H: Effect of compound preparation Tongqiao Jiannao capsules on neural cell apoptosis and Bcl-2 and Bax protein levels in a rat model of brain ischemia/reperfusion injury. Neural Regener Res 3:871-874, 2008.
29. Babu PP, Yoshida Y, Su M, Segura M, Kawamura S and Yasui N: Immunohistochemical expression of Bcl-2, Bax and cytochrome c following focal cerebral ischemia and effect of hypothermia in rat. Neurosci Lett 291:196-200, 2000.
30. Wang T, Gu J, Wu PF, et al: Protection by tetrahydroxystilbene glucoside against cerebral ischemia: involvement of JNK, SIRT1 and NF-kappaB pathways and inhibition of intracellular ROS/RNS generation. Free Radic Biol Med 47:229-240, 2009.
31. Wu HW, Li HF, Wu XY, Zhao J and Guo J: Reactive oxygen species mediate ERK activation through different Raf-1-dependent signaling pathways following cerebral ischemia. Neurosci Lett 432:83-87, 2008.
32. Wang Q, Sun AY, Simonyi A, et al: Ethanol preconditioning protects against ischemia/reperfusion-induced brain damage: Role of NADPH oxidase-derived ROS. Free Radic Biol Med 43:1048-1060, 2007.
33. Liang JM, Xu HY, Zhang XJ, Li X, Zhang HB and Ge PF: Role of mitochondrial function in the protective effects of ischaemic postconditioning on ischaemia/reperfusion cerebral damage. J Int Med Res 41:618-627, 2013.
34. Zhang L, Zhang ZG, Liu XS, Hozeska-Solgot A and Chopp M: The PI3K/Akt pathway mediates the neuroprotective effect of atorvastatin in extending thrombolytic therapy after embolic stroke in the rat. Arterioscler Thromb Vasc Biol 27:2470-2475, 2007.
35. Endo H, Nito C, Kamada H, Nishi T and Chan PH: Activation of the Akt/GSK3beta signaling pathway mediates survival of vulnerable hippocampal neurons after transient global cerebral ischemia in rats. J Cereb Blood Flow Metab 26:1479-1489, 2006.
36. Fang XX, Jiang XL, Han XH, Peng YP and Qiu YH: Neuroprotection of interleukin-6 against NMDA-induced neurotoxicity is mediated by JAK/STAT3, MAPK/ERK and PI3K/AKT signaling pathways. Cell Mol Neurobiol 33:241-251, 2013.
37. Gu R, Liu M, Wang Y, Zhou Y and He H: Angiopoietin-1 mRNA and Bcl-2 expression following estradiol treatment in ovariectomized rats with focal cerebral ischemia/reperfusion injury. Neural Regener Res 4:780-785, 2009.
38. Kitagawa K: CREB and cAMP response element-mediated gene expression in the ischemic brain. FEBS J 274:3210-3217, 2007