Increased efficiency of Campylobacter jejuni N-oligosaccharyltransferase PglB by structure-guided engineering

Open Biology

Volumn 5, Issue 4, 2015, Pages

  Ihssen, Julian ^a   Haas, Jürgen ^b,c   Kowarik, Michael ^d   Wiesli, Luzia ^a   Wacker, Michael ^d   Schwede, Torsten ^b,c   Thöny Meyer, Linda ^a  

^a SWISS FEDERAL LABORATORIES FOR MATERIALS SCIENCE AND TECHNOLOGY   (Switzerland)

^b UNIVERSITY OF BASEL   (Switzerland)

^c SWISS INSTITUTE OF BIOINFORMATICS   (Switzerland)

^d GlycoVaxyn AG   (Switzerland)

Author keywords

Campylobacter jejuni; Directed evolution; N glycosylation; Oligosaccharyltransferase; PglB; Protein modelling

Indexed keywords

BACTERIA (MICROORGANISMS); CAMPYLOBACTER JEJUNI; ESCHERICHIA COLI; PROKARYOTA; SALMONELLA ENTERICA; STAPHYLOCOCCUS AUREUS;

BACTERIAL PROTEIN; DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE - PROTEIN GLYCOTRANSFERASE; GLYCOSYLTRANSFERASE; MEMBRANE PROTEIN; OLIGOSACCHARIDE; POLYSACCHARIDE; PROTEIN BINDING;

AMINO ACID SEQUENCE; BINDING SITE; CAMPYLOBACTER JEJUNI; CHEMISTRY; COMPUTER SIMULATION; CONFORMATION; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; GLYCOSYLATION; METABOLISM; MOLECULAR GENETICS; MOLECULAR MODEL; MUTAGENESIS; MUTATION; PROCEDURES; PROTEIN ENGINEERING; PROTEIN TERTIARY STRUCTURE; SALMONELLA ENTERICA; SEQUENCE HOMOLOGY; STAPHYLOCOCCUS AUREUS; WESTERN BLOTTING;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BINDING SITES; BLOTTING, WESTERN; CAMPYLOBACTER JEJUNI; CARBOHYDRATE CONFORMATION; COMPUTER SIMULATION; ENZYME-LINKED IMMUNOSORBENT ASSAY; GLYCOSYLATION; HEXOSYLTRANSFERASES; MEMBRANE PROTEINS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS; MUTATION; OLIGOSACCHARIDES; POLYSACCHARIDES; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN STRUCTURE, TERTIARY; SALMONELLA ENTERICA; SEQUENCE HOMOLOGY, AMINO ACID; STAPHYLOCOCCUS AUREUS; SUBSTRATE SPECIFICITY;

EID: 84935004309     PISSN: None     EISSN: 20462441     Source Type: Journal    
DOI: 10.1098/rsob.140227     Document Type: Article

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