A Novel Chimeric Anti-PA Neutralizing Antibody for Postexposure Prophylaxis and Treatment of Anthrax

Scientific Reports

Volumn 5, Issue , 2015, Pages

  Xiong, Siping ^a   Tang, Qi ^a   Liang, Xudong ^c   Zhou, Tingting ^b   Yang, Jin ^a   Liu, Peng ^b   Chen, Ya ^a   Wang, Changjun ^b   Feng, Zhenqing ^a   Zhu, Jin ^a,b  

^a NANJING MEDICAL UNIVERSITY   (China)

^b Huadong Medical Institute of Biotechniques   (China)

^c CHINESE CENTER FOR DISEASE CONTROL AND PREVENTION   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ANTHRAX TOXIN; BACTERIAL ANTIGEN; BACTERIAL TOXIN; HYBRID PROTEIN; NEUTRALIZING ANTIBODY; PROTEIN BINDING;

AMINO ACID SEQUENCE; ANIMAL; ANTHRAX; BACILLUS ANTHRACIS; CELL LINE; CHEMISTRY; FEMALE; FISCHER 344 RAT; HUMAN; IMMUNOLOGY; MOLECULAR GENETICS;

AMINO ACID SEQUENCE; ANIMALS; ANTHRAX; ANTIBODIES, NEUTRALIZING; ANTIGENS, BACTERIAL; BACILLUS ANTHRACIS; BACTERIAL TOXINS; CELL LINE; FEMALE; HUMANS; MOLECULAR SEQUENCE DATA; PROTEIN BINDING; RATS, INBRED F344; RECOMBINANT FUSION PROTEINS;

EID: 84934765627     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep11776     Document Type: Article

References (38)

1. Ding, G. et al. A human/murine chimeric fab antibody neutralizes anthrax lethal toxin in vitro. Clin Dev Immunol 2013, 475809 (2013
2. Leysath, C. E. et al. Mouse monoclonal antibodies to anthrax edema factor protect against infection. Infect Immun 79, 4609-16 (2011
3. Ngundi, M. M. et al. Analysis of defined combinations of monoclonal antibodies in anthrax toxin neutralization assays and their synergistic action. Clin Vaccine Immunol 19, 731-9 (2012
4. Traggiai, E. et al. An efficient method to make human monoclonal antibodies from memory B cells: potent neutralization of SARS coronavirus. Nat Med 10, 871-5 (2004
5. Mehra, V., Khanna, H., Chandra, R. &Singh, Y. Anthrax-toxin-mediated delivery of a 19 kDa antigen of Mycobacterium tuberculosis into the cytosol of mammalian cells. Biotechnol Appl Biochem 33, 71-4 (2001
6. Barth, H., Aktories, K., Popoff, M. R. &Stiles, B. G. Binary bacterial toxins: biochemistry, biology, and applications of common Clostridium and Bacillus proteins. Microbiol Mol Biol Rev 68, 373-402, table of contents (2004
7. Froude, J. W., 2nd, Thullier, P. &Pelat, T. Antibodies against anthrax: mechanisms of action and clinical applications. Toxins (Basel) 3, 1433-52 (2011
8. Bromberg-White, J., Lee, C. S. &Duesbery, N. Consequences and utility of the zinc-dependent metalloprotease activity of anthrax lethal toxin. Toxins (Basel) 2, 1038-53 (2010
9. Bradley, K. A., Mogridge, J., Mourez, M., Collier, R. J. &Young, J. A. Identification of the cellular receptor for anthrax toxin. Nature 414, 225-9 (2001
10. Scobie, H. M., Rainey, G. J., Bradley, K. A. &Young, J. A. Human capillary morphogenesis protein 2 functions as an anthrax toxin receptor. Proc Natl Acad Sci USA 100, 5170-4 (2003
11. Pilpa, R. M., Bayrhuber, M., Marlett, J. M., Riek, R. &Young, J. A. A receptor-based switch that regulates anthrax toxin pore formation. PLoS Pathog 7, e1002354 (2011
12. Lacy, D. B. &Collier, R. J. Structure and function of anthrax toxin. Curr Top Microbiol Immunol 271, 61-85 (2002
13. Collier, R. J. &Young, J. A. Anthrax toxin. Annu Rev Cell Dev Biol 19, 45-70 (2003
14. Miller, C. J., Elliott, J. L. &Collier, R. J. Anthrax protective antigen: prepore-to-pore conversion. Biochemistry 38, 10432-41 (1999
15. Koehler, T. M. &Collier, R. J. Anthrax toxin protective antigen: low-pH-induced hydrophobicity and channel formation in liposomes. Mol Microbiol 5, 1501-6 (1991
16. Milne, J. C. &Collier, R. J. pH-dependent permeabilization of the plasma membrane of mammalian cells by anthrax protective antigen. Mol Microbiol 10, 647-53 (1993
17. Klimpel, K. R., Molloy, S. S., Thomas, G. &Leppla, S. H. Anthrax toxin protective antigen is activated by a cell surface protease with the sequence specificity and catalytic properties of furin. Proc Natl Acad Sci USA 89, 10277-81 (1992
18. Singh, Y., Chaudhary, V. K. &Leppla, S. H. A deleted variant of Bacillus anthracis protective antigen is non-toxic and blocks anthrax toxin action in vivo. J Biol Chem 264, 19103-7 (1989
19. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-5 (1970
20. Sloat, B. R., Shaker, D. S., Le, U. M. &Cui, Z. Nasal immunization with the mixture of PA63, LF, and a PGA conjugate induced strong antibody responses against all three antigens. FEMS Immunol Med Microbiol 52, 169-79 (2008
21. Casadevall, A. Passive antibody administration (immediate immunity) as a specific defense against biological weapons. Emerg Infect Dis 8, 833-41 (2002
22. Albrecht, M. T. et al. Human monoclonal antibodies against anthrax lethal factor and protective antigen act independently to protect against Bacillus anthracis infection and enhance endogenous immunity to anthrax. Infect Immun 75, 5425-33 (2007
23. Chen, Z. et al. Efficient neutralization of anthrax toxin by chimpanzee monoclonal antibodies against protective antigen. J Infect Dis 193, 625-33 (2006
24. Migone, T. S. et al. Raxibacumab for the treatment of inhalational anthrax. N Engl J Med 361, 135-44 (2009
25. Peterson, J. W. et al. Human monoclonal antibody AVP-21D9 to protective antigen reduces dissemination of the Bacillus anthracis Ames strain from the lungs in a rabbit model. Infect Immun 75, 3414-24 (2007
26. Vitale, L. et al. Prophylaxis and therapy of inhalational anthrax by a novel monoclonal antibody to protective antigen that mimics vaccine-induced immunity. Infect Immun 74, 5840-7 (2006
27. Mohamed, N. et al. A high-affinity monoclonal antibody to anthrax protective antigen passively protects rabbits before and after aerosolized Bacillus anthracis spore challenge. Infect Immun 73, 795-802 (2005
28. Staats, H. F. et al. In vitro and in vivo characterization of anthrax anti-protective antigen and anti-lethal factor monoclonal antibodies after passive transfer in a mouse lethal toxin challenge model to define correlates of immunity. Infect Immun 75, 5443-52 (2007
29. Zhao, P., Liang, X., Kalbfleisch, J., Koo, H. M. &Cao, B. Neutralizing monoclonal antibody against anthrax lethal factor inhibits intoxication in a mouse model. Hum Antibodies 12, 129-35 (2003
30. Lim, N. K. et al. An anthrax lethal factor-neutralizing monoclonal antibody protects rats before and after challenge with anthrax toxin. Infect Immun 73, 6547-51 (2005
31. Kozel, T. R. et al. Protective and immunochemical activities of monoclonal antibodies reactive with the Bacillus anthracis polypeptide capsule. Infect Immun 75, 152-63 (2007
32. Chen, Z., Moayeri, M. &Purcell, R. Monoclonal antibody therapies against anthrax. Toxins (Basel) 3, 1004-19 (2011
33. Maynard, J. A. et al. Protection against anthrax toxin by recombinant antibody fragments correlates with antigen affinity. Nat Biotechnol 20, 597-601 (2002
34. Huang, J. et al. A high-affinity human/mouse cross-reactive monoclonal antibody, specific for VEGFR-2 linear and conformational epitopes. Cytotechnology 62, 61-71 (2010
35. Kohler, G. &Milstein, C. Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur J Immunol 6, 511-9 (1976
36. Mao, Y. et al. A Novel LMP1 Antibody Synergizes with Mitomycin C to Inhibit Nasopharyngeal Carcinoma Growth in Vivo Through Inducing Apoptosis and Downregulating Vascular Endothelial Growth Factor. Int J Mol Sci 13, 2208-18 (2012
37. Little, S. F. et al. Production and characterization of monoclonal antibodies against the lethal factor component of Bacillus anthracis lethal toxin. Infect Immun 58, 1606-13 (1990
38. Chen, Z. et al. Novel chimpanzee/human monoclonal antibodies that neutralize anthrax lethal factor, and evidence for possible synergy with anti-protective antigen antibody. Infect Immun 77, 3902-08 (2009