Mechanism and method for generating tumor-free ips cells using intronic microrna mir-302 induction

Methods in Molecular Biology

Volumn 936, Issue , 2013, Pages 295-312

  Lin, Shi Lung ^a   Ying, Shao Yao ^b  

^a WJWU and LYNN Institute for Stem Cell Research   (United States)

^b UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

Author keywords

Bmi 1; Cdk2; Cdk4 6; Cdkn1a; Cell cycle; Cyclin d; Esc; Ipsc; Microrna; Mir 302; P14 p19arf; P16ink4a; P21cip1 waf1; Rnai; Stem cell; Tumor suppressor

Indexed keywords

MICRORNA; MICRORNA 302; OLIGONUCLEOTIDE; UNCLASSIFIED DRUG;

ARTICLE; CANCER INHIBITION; CARCINOGENICITY; CELL SELECTION; CELLULAR PARAMETERS; ELECTROPORATION; EXPRESSION VECTOR; GENE CLUSTER; GENE CONSTRUCT; GENE INDUCTION; GENE INSERTION; GENETIC TRANSFECTION; HUMAN; INTRON; MOLECULAR DYNAMICS; NEOPLASM; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; RNA STRUCTURE;

EID: 84934444847     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-83-0_23     Document Type: Article

References (34)

1. Lin SL, Chang D, Chang-Lin S, Lin CH, Wu DTS, Chen DT, Ying SY (2008) Mir-302 reprograms human skin cancer cells into a pluripotent ES-cell-like state. RNA 14:2115-2124
2. Lin SL, Chang D, Lin CH, Ying SY, Leu D, Wu DTS (2011) Regulation of somatic cell reprogramming through inducible mir-302 expression. Nucleic Acids Res 39:1054-1065
3. Lin SL, Chang D, Ying SY, Leu D, Wu DTS (2010) MicroRNA miR-302 inhibits the tumorigenecity of human pluripotent stem cells by coordinate suppression of CDK2 and CDK4/6 cell cycle pathways. Cancer Res 70:9473-9482
4. Li Y, Pei J, Xia H, Ke H, Wang H, Tao W (2003) Lats2, a putative tumor suppressor, inhibits G1/S transition. Oncogene 22:4398-4405
5. Ying SY, Lin SL (2004) Intron-derived microRNAs-fine tuning of gene functions. Gene 342:25-28
6. Becker KA, Ghule PN, Therrien JA, Lian JB, Stein JL, van Wijnen AJ, Stein GS (2006) Selfrenewal of human embryonic stem cells is supported by a shortened G1 cell cycle phase. J Cell Physiol 209:883-893
7. Hanna J, Saha K, Pando B, van Zon J, Lengner CJ, Creyghton MP, van Oudenaarden A, Jaenisch R (2009) Direct cell reprogramming is a stochastic process amenable to acceleration. Nature 462:595-601
8. Rowland BD, Bernards R, Peeper DS (2005) The KLF4 tumour suppressor is a transcriptional repressor of p53 that acts as a context-dependent oncogene. Nat Cell Biol 7:1074-1082
9. Jiang J, Chan YS, Loh YH, Cai J, Tong GQ, Lim CA, Robson P, Zhong S, Ng HH (2008) A core Klf circuitry regulates self-renewal of embryonic stem cells. Nat Cell Biol 10:353-360
10. Kim J, Chu J, Shen X, Wang J, Orkin SH (2008) An extended transcriptional network for pluripotency of embryonic stem cells. Cell 132:1049-1061
11. Nandan MO, Yang VW (2009) The role of Krüppel-like factors in the reprogramming of somatic cells to induced pluripotent stem cells. Histol Histopathol 24:1343-1355
12. Parry D, Bates S, Mann DJ, Peters G (1995) Lack of cyclin D-Cdk complexes in Rb-negative cells correlated with high levels of p16INK4/ MTS1 tumor suppressor gene product. EMBO J 14:503-511
13. Quelle DE, Zindy F, Ashmun RA, Sherr CJ (1995) Alternative reading frames of the NK4a tumor suppressor gene encode two unrelated proteins capable of inducing cell cycle arrest. Cell 83:993-1000
14. Kamijo T, Zindy F, Roussel MF, Quelle DE, Downing JR, Ashmun RA, Grosveld G, Sherr CJ (1997) Tumor suppression at the mouse INK4a locus mediated by the alternative reading frame product p19ARF. Cell 91:649-659
15. Chan MF, van Amerongen R, Nijjar T, Cuppen E, Jones PA, Laird PW (2001) Reduced rates of gene loss, gene silencing, and gene mutation in Dnmt1-deficient embryonic stem cells. Mol Cell Biol 21:7587-7600
16. Banito A, Rashid ST, Acosta JC, Li S, Pereira CF, Geti I, Pinho S, Silva JC, Azuara V, Walsh M, Vallier L, Gil J (2009) Senescence impairs successful reprogramming to pluripotent stem cells. Genes Dev 23:2134-2139
17. Feng Q, Lu SJ, Klimanskaya I, Gomes I, Kim D, Chung Y, Honig GR, Kim KS, Lanza R (2010) Hemangioblastic derivatives from human induced pluripotent stem cells exhibit limited expansion and early senescence. Stem Cells 28:704-712
18. Li H, Collado M, Villasante A, Strati K, Ortega S, Cañamero M, Blasco MA, Serrano M (2009) The Ink4/Arf locus is a barrier for iPS cell reprogramming. Nature 460:1136-1139
19. Utikal J, Polo JM, Stadtfeld M, Maherali N, Kulalert W, Walsh RM, Khalil A, Rheinwald JG, Hochedlinger K (2009) Immortalization eliminates a roadblock during cellular reprogramming into iPS cells. Nature 460:1145-1148
20. Marión RM, Strati K, Li H, Murga M, Blanco R, Ortega S, Fernandez-Capetillo O, Serrano M, Blasco MA (2009) A p53-mediated DNA damage response limits reprogramming to ensure iPS cell genomic integrity. Nature 460: 1149-1153
21. Won J, Yim J, Kim TK (2002) Sp1 and Sp3 recruit histone deacetylase to repress transcription of human telomerase reverse transcriptase (hTERT) promoter in normal human somatic cells. J Biol Chem 277:38230-38238
22. Zhu O, Liu C, Ge Z, Fang X, Zhang X, Straat K, Bjorkholm M, Xu D (2008) Lysine-specific demethylase 1 (LSD1) is required for the transcriptional repression of the telomerase reverse transcriptase (hTERT) gene. PLoS One 3:e1446
23. Lin SL, Chang D, Wu DY, Ying SY (2003) A novel RNA splicing-mediated gene silencing mechanism potential for genome evolution. Biochem Biophys Res Commun 310:754-760
24. Lin SL, Kim H, Ying SY (2008) Intronmediated RNA interference and microRNA (miRNA). Front Biosci 13:2216-2230
25. Barroso-delJesus A, Romero-López C, Lucena- Aguilar G, Melen GJ, Sanchez L, Ligero G, Berzal-Herranz A, Menendez P (2008) Embryonic stem cell-specific miR302-367 cluster: human gene structure and functional characterization of its core promoter. Mol Cell Biol 28:6609-6619
26. Suh MR, Lee Y, Kim JY, Kim SK, Moon SH, Lee JY, Cha KY, Chung HM, Yoon HS, Moon SY, Kim VN, Kim KS (2004) Human embryonic stem cells express a unique set of microRNAs. Dev Biol 270:488-498
27. Lin SL, Ying SY (2006) Gene silencing in vitro and in vivo using intronic microRNAs. In: Ying SY (ed) MicroRNA protocols. Humana Press, Totowa, pp 295-312
28. Lin SL, Chang D, Ying SY (2005) Asymmetry of intronic pre-microRNA structures in functional RISC assembly. Gene 356:32-38
29. Danin-Kreiselman M, Lee CY, Chanfreau G (2003) RNAse III-mediated degradation of unspliced pre-mRNAs and lariat introns. Mol Cell 11:1279-1289
30. Yi R, Qin Y, Macara IG, Cullen BR (2003) Exportin-5 mediates the nuclear export of premicroRNAs and short hairpin RNAs. Genes Dev 17:3011-3016
31. Lund E, Guttinger S, Calado A, Dahlberg JE, Kutay U (2004) Nuclear export of microRNA precursors. Science 303:95-98
32. Zhang G, Taneja KL, Singer RH, Green MR (1994) Localization of pre-mRNA splicing in mammalian nuclei. Nature 372:809-812
33. Lewis BP, Green RE, Brenner SE (2003) Evidence for the widespread coupling of alternative splicing and nonsense-mediated mRNA decay in humans. Proc Natl Acad Sci USA 100:189-192
34. Grimm D, Streetz KL, Jopling CL, Storm TA, Pandey K, Davis CR, Marion P, Salazar F, Kay MA (2006) Fatality in mice due to oversaturation of cellular microRNA/short hairpin RNA pathways. Nature 441:537-541