Baculovirus expression systems for recombinant protein production in insect cells

Recent Patents on Biotechnology

Volumn 3, Issue 1, 2009, Pages 46-54

  Hitchman, Richard B ^a   Possee, Robert D ^b   King, Linda A ^a  

^a OXFORD BROOKES UNIVERSITY   (United Kingdom)

^b CENTRE FOR ECOLOGY AND HYDROLOGY   (United Kingdom)

Author keywords

Bacmid; Baculovirus; Budded virus; High throughput and recombinant protein expression

Indexed keywords

BIOCONTROL; GENE EXPRESSION; GENE TRANSFER; RECOMBINANT PROTEINS; THROUGHPUT; VIRUSES;

BACMID; BACULOVIRUS; BACULOVIRUS EXPRESSION SYSTEMS; BUDDED VIRUS; EXPRESSION VECTORS; HIGH THROUGHPUT AND RECOMBINANT PROTEIN EXPRESSION; HIGH-THROUGHPUT; INSECT CELLS; RECOMBINANT PROTEIN EXPRESSION; RECOMBINANT PROTEIN PRODUCTIONS;

MAMMALS;

BETA GALACTOSIDASE; CHAPERONE; CHLORAMPHENICOL; DNA FRAGMENT; GENE PRODUCT; GLYCOPROTEIN; GLYCOPROTEIN GP 64; NEOMYCIN; P21 ACTIVATED KINASE 6; POLYHEDRIN; PROTEIN ACMNPV 39K; PROTEIN ACMNPV IE1; PROTEIN BCL 2; PROTEIN P VANK 1; PROTEIN P10; PROTEOME; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; VANKYRIN PROTEIN; VIRUS DNA; VIRUS VECTOR;

BACULOVIRUS EXPRESSION SYSTEM; BIOLOGICAL PEST CONTROL; CHROMATIN ASSEMBLY AND DISASSEMBLY; DNA SEQUENCE; DNA TRANSPOSITION; GENE EXPRESSION; GENE TRANSFER; HETEROLOGOUS EXPRESSION; HIGH THROUGHPUT SCREENING; INSECT CELL; NONHUMAN; PATENT; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN STRUCTURE; REVIEW; SIGNAL TRANSDUCTION; TRANSPOSON; VIRAL GENE DELIVERY SYSTEM; VIRUS PURIFICATION; VIRUS RECOMBINANT; ANIMAL; BACULOVIRUS; BIOSYNTHESIS; CELL CULTURE; GENE VECTOR; GENETIC TRANSFECTION; GENETICS; INSECT; METABOLISM;

HEXAPODA; LEPIDOPTERA; MAMMALIA;

ANIMALS; BACULOVIRIDAE; CELLS, CULTURED; GENETIC VECTORS; INSECTS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; TRANSFECTION;

EID: 62149138458     PISSN: 18722083     EISSN: None     Source Type: Journal    
DOI: 10.2174/187220809787172669     Document Type: Review

References (96)

1. Smith GE, Summers MD, Fraser MJ. Production of human beta interferon in insect cells infected with a baculovirus expression vector. Mol Cell Biol 1983; 3(12): 2156-2165.
2. Volkman LE, Summers MD. Autographa californica nuclear polyhedrosis virus: comparative infectivity of the occluded, alkali-liberated, and nonoccluded forms. J Invertebr Pathol 1977; 30(1): 102-103.
3. Volkman LE, Summers MD, Hsieh CH. Occluded and nonoccluded nuclear polyhedrosis virus grown in Trichoplusia ni: comparative neutralization comparative infectivity, and in vitro growth studies. J Virol 1976; 19(3): 820-832.
4. Grace TD. Establishment of four strains of cells from insect tissues grown in vitro. Nature 1962; 195: 788-789.
5. Gaw ZY, Liu NT, Zia TU. Tissue culture methods for cultivation of virus grasserie. Acta Virol 1959; 3(Suppl): 55-60.
6. Hink WF. Established insect cell line from the cabbage looper, Trichoplusia ni. Nature 1970; 226(5244): 466-467.
7. Goodwin RH, Vaughn JL, Adams JR, Louloudes SJ. Replication of a nuclear polyhedrosis virus in an established insect cell line. J Invertebr Pathol 1970; 16(2): 284-288.
8. Vail PV, Jay DL and Hunter DK. Cross infectivity of a nuclear polyhedrosis virus isolated from the alfalfa looper Autographa californica. In Proceedings, 4th International Colloquium Insect Pathology; 1971 25-281970; College Park, MD; 1971; 297-304.
9. Harrap KA. The structure of nuclear polyhedrosis viruses. 3. Virus assembly. Virology 1972; 50(1): 133-139.
10. Harrap KA. The structure of nuclear polyhedrosis viruses. II. The virus particle. Virology 1972; 50(1): 124-132.
11. Harrap KA. The structure of nuclear polyhedrosis viruses. I. The inclusion body. Virology 1972; 50(1): 114-123.
12. Hink WF, Strauss EM. Plaque assay of alfalfa looper nuclear polyhedrosis virus on the TN-368 cell line methods in cell science 1979; 5(1): 1033-1035.
13. Vail P, Jay DL, Hink WF. Replication and infectivity of the nuclear polyhedrosis virus of the Alfalfa Looper,Autographa californica, produced in cells grown in vitro. J Invert Pathol 1973; 22: 231-237.
14. Wood HA. An agar overlay plague assay method for Autographa californica nuclear polyhedrosis virus. J Invert Pathol 1977; 29: 304-307.
15. Rohrmann GF, Bailey TJ, Brimhall B, Becker RR, Beaudreau GS. Tryptic peptide analysis and NH2-terminal amino acid sequences of polyhedrins of two baculoviruses from Orgyia pseudotsugata. Proc Natl Acad Sci USA 1979; 76(10): 4976-4980.
16. Serebryani SB, Levitina TL, Kautsman ML, Radavski YL, Gusak NM, Ovander MN, Sucharenko NV, Kozlov EA. The primary structure of the polyhedral protein of nuclear polyhedrosis virus (NPV) of Bombyx mori J Invertebr Path 1977; 30(3): 442-443.
17. Kozlov EA, Levitina TL, Gusak NM, and Serebryani SB. Comparison of polyhedrin protein amino acid sequences. Bioorg Khim 1981; 7: 1008-1015.
18. Rohrmann GF, Leisy DJ, Chow KC, Pearson GD, Beaudreau GS. Identification, cloning, and R-loop mapping of the polyhedrin gene from the multicapsid nuclear polyhedrosis virus of Orgyia pseudotsugata. Virology 1982; 121(1): 51-60.
19. Smith GE, Vlak JM, Summers MD. Physical analysis of Autographa californica nuclear polyhedrosis virus transcripts for polyhedrin and 10,000-molecular-weight protein. J Virol 1983; 45(1): 215-225.
20. Hooft van Iddekinge BJ, Smith GE, Summers MD. Nucleotide sequence of the polyhedrin gene of Autographa californica nuclear polyhedrosis virus. Virology 1983; 131(2): 561-565.
21. Smith GE, Fraser MJ, Summers MD. Molecular Engineering of the Autographa californica Nuclear polyhedrosis virus genome: deletion mutations within the polyhedrin gene. J Virol 1983; 46(2): 584-593.
22. Smith GE, Ju G, Ericson BL, Moschera J, Lahm HW, Chizzonite R, et al. Modification and secretion of human interleukin 2 produced in insect cells by a baculovirus expression vector. Proc Natl Acad Sci USA 1985; 82(24): 8404-8408.
23. Pennock GD, Shoemaker C, Miller LK. Strong and regulated expression of Escherichia coli beta-galactosidase in insect cells with a baculovirus vector. Mol Cell Biol 1984; 4(3): 399-406.
24. Summers MD. Milestones leading to the genetic engineering of baculoviruses as expression vector systems and viral pesticides. Adv Virus Res 2006; 68: 3-73.
25. Smith, G.E., Summers, M.D.: US4745051 (1988).
26. Smith, G.E., Summers, M.D.: US4879236 (1989).
27. Luckow VA, Summers MD. Signals important for high-level expression of foreign genes in Autographa californica nuclear polyhedrosis virus expression vectors. Virology 1988; 167(1): 56-71.
28. Miller LK. Baculoviruses for foreign gene expression in insect cells. Biotechnology 1988; 10: 457-465.
29. Davis TR, Trotter KM, Granados RR, Wood HA. Baculovirus expression of alkaline phosphatase as a reporter gene for evaluation of production, glycosylation and secretion. Biotechnology (N Y). 1992; 10(10): 1148-1150.
30. Kakker NK, Mikhailov MV, Nermut MV, Burny A, Roy P. Bovine leukemia virus Gag particle assembly in insect cells: formation of chimeric particles by domain-switched leukemia/lentivirus Gag polyprotein. Virology 1999; 265(2): 308-318.
31. James DC, Freedman RB, Hoare M, Ogonah OW, Rooney BC, Larionov OA, et al. N-Glycosylation of recombinant human interferon-gamma produced in different animal expression systems. Biotechnology (NY) 1995; 13(6): 592-596.
32. Hericourt F, Blanc S, Redeker V, Jupin I. Evidence for phosphorylation and ubiquitinylation of the turnip yellow mosaic virus RNA-dependent RNA polymerase domain expressed in a baculovirus-insect cell system. Biochem J 2000; 349(Pt 2): 417-425.
33. Hodder AN, Crewther PE, Matthew ML, Reid GE, Moritz RL, Simpson RJ, et al. The disulfide bond structure of Plasmodium apical membrane antigen-1. J Biol Chem 1996; 271(46): 29446-29452.
34. Luckow V. Baculovirus Expression Systems and Biopesticides. New York: Wiley-Liss, Inc; 1995.
35. Hu YC. Baculoviral vectors for gene delivery: A review. Curr Gene Ther 2008; 8(1): 54-65.
36. Kost TA, Condreay JP, Ames RS, Rees S, Romanos MA. Implementation of BacMam virus gene delivery technology in a drug discovery setting. Drug Discov Today 2007; 12(9-10): 396-403.
37. Garzia L, Andre A, Amoresano A, et al. Method to express and purify nm23-H2 protein from baculovirus-infected cells. Biotechniques 2003; 35(2): 384-388.
38. Leder L, Freuler F, Forstner M, Mayr LM. New methods for efficient protein production in drug discovery. Curr Opin Drug Discov Devel 2007; 10(2): 193-202.
39. Chambers SP, Austen DA, Fulghum JR, Kim WM. High-throughput screening for soluble recombinant expressed kinases in Escherichia coli and insect cells. Protein Expr Purif 2004; 36(1): 40-47.
40. Ayres MD, Howard SC, Kuzio J, Lopez-Ferber M, Possee RD. The complete DNA sequence of Autographa californica nuclear polyhedrosis virus. Virology 1994; 202(2): 586-605.
41. Bishop, D., Possee, R., Ayres, M.: WO9601320 (1996).
42. Kitts PA, Ayres MD, Possee RD. Linearization of baculovirus DNA enhances the recovery of recombinant virus expression vectors. Nucleic Acids Res 1990; 18(19): 5667-5672.
43. Kitts PA, Possee RD. A method for producing recombinant baculovirus expression vectors at high frequency. Biotechniques 1993; 14 (5): 810-817.
44. Lee, S.C., Leusch, M.S., Luckow, V.A., Olins, P.O.: US5348886 (1994).
45. Luckow VA, Lee SC, Barry GF, Olins PO. Efficient generation of infectious recombinant baculoviruses by site-specific transposon-mediated insertion of foreign genes into a baculovirus genome propagated in Escherichia coli. J Virol 1993; 67(8): 4566-4579.
46. Bac-to Bac Baculovirus Expression System. Version D ed: Invitrogen Life Technologies 2004.
47. Anderson D, Harris R, Polayes D, Ciccarone V, Donahue R, Gerard G. and Jessee J. Rapid generation of recombinant baculoviruses and expression of foreign genes using the Bac-to-Bac® baculovirus expression system. Focus 1996; 17: 53-58.
48. Hunt I. From gene to protein: A review of new and enabling technologies for multi-parallel protein expression. Protein Expr Purif 2005; 40(1): 1-22.
49. Polayes D, Harris R, Anderson D and Ciccarone V. New baculovirus expression vectors for the purification of recombinant proteins from insect cells. Focus 1996; 18: 10-13.
50. Harris R, and Polayes, D. A new baculovirus expression vector for the simultaneous expression of two heterologous proteins in the same insect cell. Focus 1997; 19: 6-8.
51. Lalonde S, Ehrhardt DW, Loque D, Chen J, Rhee SY, Frommer WB. Molecular and cellular approaches for the detection of protein-protein interactions: latest techniques and current limitations. Plant J 2008; 53(4): 610-635.
52. Berger I, Fitzgerald DJ, Richmond TJ. Baculovirus expression system for heterologous multiprotein complexes. Nat Biotechnol 2004; 22(12): 1583-1587.
53. Berger, I., Richmond, T.J.: WO20055085456 (2005).
54. Fitzgerald DJ, Berger P, Schaffitzel C, Yamada K, Richmond TJ, Berger I. Protein complex expression by using multigene baculoviral vectors. Nat Methods 2006; 3(12): 1021-1032.
55. Possee, R.D., King, L.A.: WO0112829 (2001).
56. Possee RD, Hitchman RH, Richards KS, et al. Generation of baculovirus vectors for the high throughput production of proteins in insect cells. Biotechnol Bioeng 2008; 101(6): 1115-1122.
57. Novy, R., Monsma, S.: US20036589783 (2003).
58. Novy R, Yaeger K, Monsma S, Scott M. pTriExτm-1 Multisystem Vector for protein expression in E. coli, mammalian, and insect cells. Innovations 1999; 10: 1-4.
59. Haun RS, Serventi IM, Moss J. Rapid, reliable ligation-independent cloning of PCR products using modified plasmid vectors. Biotechniques 1992; 13(4): 515-518.
60. Aslanidis C, de Jong PJ. Ligation-independent cloning of PCR products (LIC-PCR). Nucleic Acids Res 1990; 18(20): 6069-6074.
61. Elledge, S.: US20070292954 (2007).
62. Farmer, A.: US2003054555 (2003).
63. Hamilton MD, Nuara AA, Gammon DB, Buller RM, Evans DH. Duplex strand joining reactions catalyzed by vaccinia virus DNA polymerase. Nucleic Acids Res 2007; 35(1): 143-151.
64. Clontech Laboratories I. In-fusion user manual; 2007.
65. Farmer, A.: US20026410317 (2002).
66. Farmer, A.: US2003059900 (2003).
67. ClontechniquesXI. Creator DNA & expression cloning system 2000.
68. Midgett CR, Madden DR. Breaking the bottleneck: Eukaryotic membrane protein expression for high-resolution structural studies. J Struct Biol 2007; 160(3): 265-274.
69. Ailor E, Betenbaugh MJ. Modifying secretion and posttranslational processing in insect cells. Curr Opin Biotechnol 1999; 10(2): 142-145.
70. Yun EY, Goo TW, Kim SW, et al. Changes in cellular secretory processing during baculovirus infection. Biotechnol Lett 2005; 27(14): 1041-1045.
71. Belyaev, A.: US20077226781 (2007).
72. Guarino, L., Jarvis, D.: US5077214 (1991).
73. Guarino, L., Jarvis, D.: US5162222 (1992).
74. Rodems SM, Friesen PD. The hr5 transcriptional enhancer stimulates early expression from the Autographa californica nuclear polyhedrosis virus genome but is not required for virus replication. J Virol 1993; 67(10): 5776-5785.
75. Pullen SS, Friesen PD. Early transcription of the ie-1 transregulator gene of Autographa californica nuclear polyhedrosis virus is regulated by DNA sequences within its 5' noncoding leader region. J Virol 1995; 69(1): 156-165.
76. Jarvis DL, Weinkauf C, Guarino LA. Immediate-early baculovirus vectors for foreign gene expression in transformed or infected insect cells. Protein Expr Purif 1996; 8(2): 191-203.
77. Loomis KH, Yaeger KW, Batenjany MM, et al. Insect-Direct System: rapid, high-level protein expression and purification from insect cells. J Struct Funct Genomics 2005; 6(2-3): 189-194.
78. Loomis KH, Batenjany M, Bibikov S, Novy R, Bartnicki D, Wong S. The InsectDirectτm System: rapid, high-level expression and purification of proteins from insect cells. Innovations 2004; 19: 10-12.
79. Loomis KH, Novy R, Yaeger K. A novel high-level transient protein expression and purification system for Sf9 insect cells that facilitates automated screening. Innovations 2002; 16: 7-10.
80. Loomis K, Rockwell C, Sternard H, Novy R. New pIEx/Bacτm dual-purpose vectors-unique flexibility for expression in insect cells. Innovations 2007; 25: 14-17.
81. Webb, B., Kroemer, J.: US20060134743 (2006).
82. Fath-Goodin A, Kroemer J, Martin S, Reeves K, Webb BA. Polydnavirus genes that enhance the baculovirus expression vector system. Adv Virus Res 2006; 68: 75-90.
83. Kroemer JA, Webb BA. Divergences in protein activity and cellular localization within the Campoletis sonorensis Ichnovirus Vankyrin family. J Virol 2006; 80(24): 12219-12228.
84. Lin G, Li G, Granados RR, Blissard GW. Stable cell lines expressing baculovirus P35: resistance to apoptosis and nutrient stress, and increased glycoprotein secretion. In Vitro Cell Dev Biol Anim 2001; 37(5): 293-302.
85. Alnemri ES, Robertson NM, Fernandes TF, Croce CM, Litwack G. Overexpressed full-length human BCL2 extends the survival of baculovirus-infected Sf9 insect cells. Proc Natl Acad Sci USA 1992; 89(16): 7295-7299.
86. Mitchell-Logean C and Murhammer DW. bcl-2 expression in Spodoptera frugiperda Sf-9 and Trichoplusia Ni BTI-Tn-5B1-4 insect cells: Effect on recombinant protein expression and cell viability. Biotechnol Bioeng 1997; 56(4): 380-390.
87. Dixit, V., Hamilton, R.W., Goor, J. van de.: US20040002139 A1 (2004).
88. Lin G, Li G, Granados RR, Blissard GW. Stable cell lines expressing baculovirus P35: resistance to apoptosis and nutrient stress, and increased glycoprotein secretion. in Vitro Cell Dev Biol Anim 2001; 37(5): 293-302.
89. Marchal I, Jarvis DL, Cacan R, Verbert A. Glycoproteins from insect cells: sialylated or not? Biol Chem 2001; 382(2): 151-159.
90. Szkudlinski MW, Thotakura NR, Tropea JE, Grossmann M, Weintraub BD. Asparagine-linked oligosaccharide structures determine clearance and organ distribution of pituitary and recombinant thyrotropin. Endocrinology 1995; 136(8): 3325-3330.
91. Grossmann M, Wong R, Teh NG, et al. Expression of biologically active human thyrotropin (hTSH) in a baculovirus system: effect of insect cell glycosylation on hTSH activity in vitro and in vivo. Endocrinology 1997; 138(1): 92-100.
92. Jarvis, D., Beek, N.V., Fraser, M.: US20070067855 (2007).
93. Hollister JR, Shaper JH, Jarvis DL. Stable expression of mammalian beta 1,4-galactosyltransferase extends the N-glycosylation pathway in insect cells. Glycobiology 1998; 8(5): 473-80.
94. Hollister JR, Jarvis DL. Engineering lepidopteran insect cells for sialoglycoprotein production by genetic transformation with mammalian beta 1,4-galactosyltransferase and alpha 2,6-sialyltransferase genes. Glycobiology 2001; 11(1): 1-9.
95. Chao, Y.-C.: US20016245528 (2001).
96. Lee JC, Chen HH, Chao YC. Persistent baculovirus infection results from deletion of the apoptotic suppressor gene p35. J Virol 1998; 72(11): 9157-9165.