Cell-associated degradation affects the yield of secreted engineered and heterologous proteins in the Bacillus subtilis expression system

Microbiology

Volumn 146, Issue 10, 2000, Pages 2583-2594

  Jensen, C L ^a   Stephenson, K ^a   Jorgensen, S T ^a   Harwood, C ^a  

^a NOVO NORDISK A S   (Denmark)

Author keywords

Bacillus subtilis; Chimeric proteins; Industrial enzymes; Protein secretion; Proteolysis

Indexed keywords

AMYLASE; BACTERIAL PROTEIN; CHIMERIC PROTEIN; RNA;

ARTICLE; BACILLUS LICHENIFORMIS; BACILLUS SUBTILIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME SYNTHESIS; GENE CONSTRUCT; HOST; IN VITRO STUDY; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN SECRETION; PROTEIN STABILITY; RNA SPLICING; TECHNIQUE;

BACILLUS LICHENIFORMIS; BACILLUS SUBTILIS;

EID: 0033754045     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-146-10-2583     Document Type: Article

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